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National Repository of Grey Literature

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	Virus cleaning on sucrose gradient after polyethylene glycol (PEG) precipitation Kubíček, O. ; Kubíčková, Z. ; Rosenbergová, K. ; Horká, Marie First centrifugation removes bigger impurities. Viruses contained in supernatant will be precipitated PEG and NaCl. Precipitated viruses settled already in acceleration about 10000g. The impurities with different density will be removed on the sucrose gradient. Detailed record
	Using of divergent flow isoelectric focusing and capillary liquid Duša, Filip ; Moravcová, Dana ; Kahle, Vladislav ; Šlais, Karel Separation of analytes from complex matrices play important role in their further identification. In this article we propose two-dimensional separation method of bovine serum albumin tryptic peptides. At the first dimension peptides are separated by divergent flow isoelectric focusing followed by reverse-phase liquid chromatography as the second dimension. Due to composition of input mixture it is possible to use autofocusing mode in first dimension. In this mode peptides are focused without addition of carrier ampholytes. Chromatograms from the second dimension were processed into contour map. Acquired data showed good separation efficiency of isoelectric focusing. Autofocusing mode enabled better resolution of peptides with close isoelectric points. Detailed record
	Differentiation of Candida strains by capillary HPLC Vykydalová, Marie ; Moravcová, Dana ; Horká, Marie ; Růžička, F. ; Kahle, Vladislav The aim of this study was to compare chromatographic profiles of lysate of Candida parapsilosis positive and Candida parapsilosis negative using capilary high performance liquid chromatography. Detailed record

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