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3D vizualizace velkých biologických vzorků nasnímaných konfokálním mikroskopem
Čapek, Martin ; Janáček, Jiří ; Karen, Petr ; Kubínová, Lucie ; Smrčka, P. ; Hána, K.
Digital volume reconstruction is a technique for rendering and visualization of a biological specimen which is greater than the field of view of a used optical instrument - a confocal laser scanning microscope in our case. Prior to the volume reconstruction, large biological specimens are cut to thin physical slices. The first step of volume reconstruction is acquisition of sets of digital volume images (spatial tiles which overlap) from all studied physical slices. The second step is composition of neighbouring spatial tiles of the same physical slice. The third reconstruction step is registration and merging of digital volumes of neighbouring physical slices of the specimen. The resulting large digital volumes are rendered and visualized using a VolumePro hardware board that offers real-time 3D volume rendering. In this paper we show a reconstruction of a chick embryonic kidney
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Stereologická metoda pro odhad povrchu tylakoidů chloroplastu
Kubínová, Lucie ; Kutík, J.
The stereological method using “local vertical windows” applied to the estimation of thylakoid surface area in the chloroplast volume, based on evaluation of chloroplast electron micrographs is presented. The method is demonstrated on the study of chloroplast ultrastructure in the leaves of plants of CE704 maize (Zea mays L.) line, developing in control and chilling conditions
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Vizualizace velkých biologických tkáňových vzorků s použitím laserové konfokální mikroskopie
Čapek, Martin ; Kubínová, Lucie ; Hána, K. ; Smrčka, P.
In biology there is often necessary to visualize a biological specimen which size is greater when compared with the field of view of a used optical acquisition instrument. The visualization of such the specimen can be achieved by volume reconstruction. We study biological specimens by using a confocal laser scanning microscope which is able to capture a digital volume representation of the specimen. We investigate great specimens containing, for example, a human tooth pulp, an epithelial layer and a vascular bed of chick embryonic gut or chick embryonic kidneys
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