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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Vyhledávání jaderného transportinu faktoru ISW1 remodelujícího chromatin u Saccharomyces cerevisiae
 Strádalová, Vendula ; Hašek, Jiří ; Janatová, Ivana
Several intragenously-tagged versions of the ISW1 gene were constructed to follow Isw1p localization either in living (GFP-fusions) or in fixed cells (c-Myc-fusions). The whole set of available importin mutants was transformed with vectors carrying an intragenously-tagged ISW1 gene. In the strains where the importins Kap120p, Nmd5p, Sxm1p, Kap114p, Los1p, Msn5p, Kap123p and Pdr6 were deleted, the Isw1p-GFP fusion regulated by the ISW1 promoter was localized always in the nucleus. This suggests that none from these importins is required for nuclear localization of Isw1p. The localization of the Isw1p-GFP in the mtr10-1 strain differed from that observed in all the other analyzed importin mutants. Our results indicate that Mtr10p could be a good candidate for Isw1p importin.
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