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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
The use of prototype of multichannel device for electrochemical detection of DNA hybridization
 Brázdilová, P. ; Navrátil, Tomáš ; Josypčuk, Bohdan ; Fojta, Miroslav
The article presents newly constructed 8-channel device, which enables sequential analysis realized on up to 8 different or equal sensors. The device is computer controlled. The screen-printed carbon electrodes (SPCE) were connected to the device. DNA hybridization was detected. DNA present in the sample spontaneously adsorbed on the surface of SPCE and free surface was subsequently blocked using beef serum albumin. The adsorbed DNA was then hybridized with the probe marked with biotin and streptavidin conjugated with enzyme alkaline phosphatase (SALP). This enzyme catalyzes the transformation of electrochemically inactive 1-naphtylphosphateone 1-naphtol, which anodic signal was detected DC voltammetry (DCV).
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