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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Spliceosomal Assembly on Precursor mRNA Takes Place in the Nuclear Speckles
 Melčák, Ivo ; Cermanová, Štěpánka ; Raška, Ivan
Nuclear speckles (speckles), enriched in splicing factors, form a distict nuclear compartment within the interchromatin space. It has been schown to serve vicinal active genes as a reservoir of splicing factors.We show that (pre-)spliceosomal assemblyy on pre-mRNA is associated with the speckles in HeLa cells.
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Transport of ribosomal RNA within the nucleolus
 Staněk, David ; Koberna, Karel ; Pliss, Artem ; Čtrnáctá, Vlasta ; Malínský, Jan ; Mašata, Martin ; Večeřová, Jaromíra ; Raška, Ivan
In the present study, we have explored the fact that incorporation of BrU into prerRNA did not interfere with pre-rRNA processing (11) and we have investigated the transport of non - isotopically labeled RNA within the nucleolus by means of transmissiion electron microscopy and confocal laser scanning microscopy.Within 20 minutes, BrU - labeled nucleolar RNA moved from the transcription sites in nucleolar DFC to GC. Double localization of bromouridine labeled RNA and fibrillarin revealed that only a ppart of the fibrillarin rich domains were transcriptionally active.
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