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Histopathological analysis of reproductive organs of male rats after short-term administration of diethyl phtalate.
Žabová, Kristýna ; Herink, Josef (referee) ; Semecký, Vladimír (advisor)
Bc. Kristýna Žabová Histopathological analysis of rat male reproductive organs after short - term application of diethyl phthalate Diploma work Charles University in Prague, Faculty of Pharmacy in Hradec Králové Study programme: Special worker in laboratory methods Background The aim of our experiment was extend, if diethyl phthalate can cause changes in spermatogenesis of rat male reproductive organs after short - term application. Methods The experiment was effected on Wistar rat males, by which was handed up diethyl phthalate by a single application. Rats were separated into two groups after five animals. To first group, group C, was intraperitoneally applied diethyl phthalate Oekanal 10 mg diluted with 1 ml aqua pro injectione and 1 ml 100% ethanol. Group C was handed up 0,4 ml of this mixture. To second group, group D, was handed up diethyl phthalate Pestanal 1g by subcutaneous injection in the amount of 0,2 ml. After 12 days were animals euthanized and their organs were sampled. Their size and weight approached physiological parameters. From the organs were made the histological preparations, which were evaluated by the light microscopy. Results In hepatic parenchyma were found different sizes of Kupfer's cells cores and congested sinusoids. In the preparation of kidneys we observed prominent...
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