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Antitumour efficacy of gels containing liposomes with hydroxy-aluminium phthalocyanine in photodynamic therapy
Kobrle, Pavel ; Poučková, Pavla (advisor) ; Štukavec, Jan (referee)
The targets of the diploma thesis presented here were to establish the antitumour efficacy of a new liposomal product with hydroxy-aluminium phthalocyanine (PC) in photodynamic therapy (PDT) of malignant tumours and determine the optimum time interval between the topical application of the product and PDT (tumour exposure to light). Effects were examined of the PC concentration on the PDT efficacy in a colon carcinoma cell line (SW-620), mammary carcinoma cell line (MDA-MB-231) and human neuroblastoma cell line (UKF NB-3). Hydroxy-aluminium phthalocyanine was employed as a photosensitizer. Outbred athymic female nude CD-1 mice already bearing the above mentioned xenotransplanted tumours were used in the experiments. The area comprising the tumour was exposed to light from a xenon lamp (600-700 nm, 80 J/cm2 ). The results were statistically evaluated, summarized in tables and plotted in graphs. In the diploma work, the liposomal product containing phthalocyanine (9-18 mg/ml) was shown to have considerable antitumour effects in the types of tumours chosen. The optimum time interval for the PDT is of 10 min after the product topical application. Key words: photodynamic therapy; hydroxy-aluminium phthalocyanine; liposomal drug delivery
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Non-canonical Bioenergetics of the Cell
Smolková, Katarína ; Ježek, Petr (advisor) ; Štukavec, Jan (referee) ; Lukeš, Julius (referee)
Cancer cells generally present abnormal bioenergetic properties including an elevated glucose uptake, a high glycolysis and a poorly efficient oxidative phosphorylation system. However, the determinants of cancer cells metabolic reprogramming remain unknown. The main question in this project was how environmental conditions in vivo can influence functioning of mitochondrial OXPHOS, because details of mitochondrial bioenergetics of cancer cells is poorly documented. We have combined two conditions, namely glucose and oxygen deprivation, to measure their potential interaction. We examined the impact of glucose deprivation and oxygen deprivation on cell survival, overall bioenergetics and OXPHOS protein expression. As a model, we have chosen a human breast carcinoma (HTB-126) and appropriate control (HTB-125) cultured cells, as large fraction of breast malignancies exhibit hypoxic tumor regions with low oxygen concentrations and poor glucose delivery. (...) Apoptosis is a natural, genetically controlled process of cell elimination. The mechanisms of its activation and regulation is a fundamental scientific question and growing body of evidence reveal further molecular pathways of apoptotic machinery. The well-known caspase activation cascade along with pro- and anti-apoptotic members of BCL family is the basic...
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Antitumour efficacy of gels containing liposomes with hydroxy-aluminium phthalocyanine in photodynamic therapy
Kobrle, Pavel ; Poučková, Pavla (advisor) ; Štukavec, Jan (referee)
The targets of the diploma thesis presented here were to establish the antitumour efficacy of a new liposomal product with hydroxy-aluminium phthalocyanine (PC) in photodynamic therapy (PDT) of malignant tumours and determine the optimum time interval between the topical application of the product and PDT (tumour exposure to light). Effects were examined of the PC concentration on the PDT efficacy in a colon carcinoma cell line (SW-620), mammary carcinoma cell line (MDA-MB-231) and human neuroblastoma cell line (UKF NB-3). Hydroxy-aluminium phthalocyanine was employed as a photosensitizer. Outbred athymic female nude CD-1 mice already bearing the above mentioned xenotransplanted tumours were used in the experiments. The area comprising the tumour was exposed to light from a xenon lamp (600-700 nm, 80 J/cm2 ). The results were statistically evaluated, summarized in tables and plotted in graphs. In the diploma work, the liposomal product containing phthalocyanine (9-18 mg/ml) was shown to have considerable antitumour effects in the types of tumours chosen. The optimum time interval for the PDT is of 10 min after the product topical application. Key words: photodynamic therapy; hydroxy-aluminium phthalocyanine; liposomal drug delivery
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Nursing care of the patient with the colon cancer
Syrová, Zuzana ; Vytejčková, Renata (advisor) ; Štukavec, Jan (referee)
This thesis is concieved as a case study, and provides complex clinical and nursing insight of a patient with colon cancer. The thesis is divided into clinical part and nursing part. The clinical part describes the anatomy and physiology of colon, disease characteristics, diagnosis, treatment options, rehabilitation, prevention, basic information about the patient, and pharmacotherapy. Nursing process is characterized in the nursing part and then there is applied model of functioning health by Majory Gordon and then care plan is established including realization and evaluation. Furthermore, patient's psychosocial state and educational process is summarized. Czech republic occupies first places in the global statistics of malignant disease of the colon, just because of it a program of early detection of this disease was initiated, this program is intended especially to risky group of citizens over 50 years. Prevention and early diagnosis is very important. Early detection and sufficiency of information can significantly affect the lives of patients.
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Non-canonical Bioenergetics of the Cell
Smolková, Katarína ; Ježek, Petr (advisor) ; Štukavec, Jan (referee) ; Lukeš, Julius (referee)
Cancer cells generally present abnormal bioenergetic properties including an elevated glucose uptake, a high glycolysis and a poorly efficient oxidative phosphorylation system. However, the determinants of cancer cells metabolic reprogramming remain unknown. The main question in this project was how environmental conditions in vivo can influence functioning of mitochondrial OXPHOS, because details of mitochondrial bioenergetics of cancer cells is poorly documented. We have combined two conditions, namely glucose and oxygen deprivation, to measure their potential interaction. We examined the impact of glucose deprivation and oxygen deprivation on cell survival, overall bioenergetics and OXPHOS protein expression. As a model, we have chosen a human breast carcinoma (HTB-126) and appropriate control (HTB-125) cultured cells, as large fraction of breast malignancies exhibit hypoxic tumor regions with low oxygen concentrations and poor glucose delivery. (...) Apoptosis is a natural, genetically controlled process of cell elimination. The mechanisms of its activation and regulation is a fundamental scientific question and growing body of evidence reveal further molecular pathways of apoptotic machinery. The well-known caspase activation cascade along with pro- and anti-apoptotic members of BCL family is the basic...
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Preclinical assesment of phthalocyanine for photodynamic therapy of rectal carc inoma
Štukavec, Jan ; Horák, Ladislav (advisor) ; Beneš, Jiří (referee) ; Dítě, Petr (referee) ; Jelínková, Helena (referee)
The present work includes research photodynamic therapy (PDT) colorectal carcinoma. The actual experiment was conducted in collaboration with the Institute of Biophysics and Informatics 1st in 2005-2008. Colorectal cancer is the most common malignancy of the Czech population. Risk of disease of colorectal cancer in the Czech population reaches 5.9% and still growing. Still is a large group patients with advanced cancer (approximately 30% of patients), which is indicated only palliative therapy. One of the most common procedure in patients with advanced cancer is the foundation of the stoma, which significantly extends the life of the patient. Photodynamic therapy is already used by default. We focused on the possibility of treatment of colorectal cancer phthalocyanine, which is among photosensitisers second generation with a rapid uptake in the tumor specimen. The aim of current research is to improve screening and to design new treatments that would achieve better results than existing procedures. PDT can improve the quality of life and in patients with advanced malignant disease and prolong survival interval.
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