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Voltammetric Determination of Metronidazol at a Polished Silver Solid Amalgam Composite Electrode
Škvorová, Lucie ; Barek, Jiří (advisor) ; Zima, Jiří (referee)
The aim of this work was to find out the optimal conditions for voltammetric determination of metronidazole, which is used as an anti-tumor drug. The stability of stock solution of metronidazole in deionized water was monitored using UV-VIS absorption spectrophotometry. Optimum conditions for voltammetric determination of metronidazole at a polished silver solid amalgam composite electrode (p-AgSA-CE) have been investigated. The dependence of voltammetric behavior of metronidazole (1∙10-4 mol/L) on pH of analyzed solution has been investigated using DC voltammetry (DCV) and differential pulse voltammetry (DPV) in the pH range 2,1 - 12,1 in aqueous media of Britton- Robinson (BR) buffer. For electrochemically reducible nitro group of metronidazole, the mechanism of voltammetric reduction at p-AgSA-CE was proposed. The optimum medium of aqueous BR buffer pH 4.0 was chosen for measuring the concentration dependences for both voltammetric techniques. The concentration range 2∙10-6 - 1∙10-4 mol/L of metronidazole was measured using DCV and DPV at p-AgSA-CE with reached limits of quantification (LQs) 2∙10-6 mol/L and 4∙10-6 mol/L for DCV and DPV, respectively. For comparison of used voltammetric techniques with supplemental analytical method, the concentration range 2∙10-6 - 1∙10-4 mol/L of metronidazole...
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Voltammetric Determination of Selected Nitroimidazole Drugs
Škvorová, Lucie ; Vyskočil, Vlastimil (advisor) ; Fischer, Jan (referee)
The aim of presented Diploma Thesis was to study an electrochemical behavior of nitroimidazole drugs metronidazole and ornidazole and to find optimal conditions for their voltammetric determination at a mercury meniscus modified silver solid amalgam electrode using DC voltammetry (DCV) and differential pulse voltammetry (DPV). Voltammetric behavior of selected drugs was investigated in dependence on the pH of the medium used (realized using a Britton-Robinson buffer (BR buffer)) and a mechanism of the reduction of both drugs was investigated using cyclic voltammetry (CV). The optimum medium for voltammetric determination of studied nitroimidazole drugs at the m-AgSAE in a region of cathodic potentials was found to be the BR buffer of pH 8.0. Then, the concentration dependences were measured in this optimum medium in the concentration range from 2·10-7 mol/L to 1·10-4 mol/L. The limits of quantification (LQs) for both metronidazole and ornidazole were found in the concentration order of 10-7 mol/L by using DCV and DPV at the m-AgSAE. The applicability of the newly developed voltammetric methods of the determination of nitroimidazole drugs was verified on the model samples of drinking and river water, with LQ ≈ 2·10-7 mol/L for both DC voltammetry and differential pulse voltammetry at the m-AgSAE....
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Voltammetric Determination of Metronidazol at a Polished Silver Solid Amalgam Composite Electrode
Škvorová, Lucie ; Zima, Jiří (referee) ; Barek, Jiří (advisor)
The aim of this work was to find out the optimal conditions for voltammetric determination of metronidazole, which is used as an anti-tumor drug. The stability of stock solution of metronidazole in deionized water was monitored using UV-VIS absorption spectrophotometry. Optimum conditions for voltammetric determination of metronidazole at a polished silver solid amalgam composite electrode (p-AgSA-CE) have been investigated. The dependence of voltammetric behavior of metronidazole (1∙10-4 mol/L) on pH of analyzed solution has been investigated using DC voltammetry (DCV) and differential pulse voltammetry (DPV) in the pH range 2,1 - 12,1 in aqueous media of Britton- Robinson (BR) buffer. For electrochemically reducible nitro group of metronidazole, the mechanism of voltammetric reduction at p-AgSA-CE was proposed. The optimum medium of aqueous BR buffer pH 4.0 was chosen for measuring the concentration dependences for both voltammetric techniques. The concentration range 2∙10-6 - 1∙10-4 mol/L of metronidazole was measured using DCV and DPV at p-AgSA-CE with reached limits of quantification (LQs) 2∙10-6 mol/L and 4∙10-6 mol/L for DCV and DPV, respectively. For comparison of used voltammetric techniques with supplemental analytical method, the concentration range 2∙10-6 - 1∙10-4 mol/L of metronidazole...
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