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National Repository of Grey Literature

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Expression and characterization of selected never-born proteins Šimonová, Johana ; Hlouchová, Klára (advisor) ; Pavlíček, Jiří (referee) This bachelor thesis deals with the study of random polypeptide sequences and occurence of secondary structure in random sequence space. Sequences for experimental characterization were selected from library containing 104 random sequences on the basis of (i) either predictions of secondary structure and disorder occurence and prediciton of solubility or (ii) a completely random selection. Eight proteins studied in this thesis belong to the second option - i.e. they were selected from the in silico generated library completely randomly. First, expression and solubility was analysed for these eight proteins. In addtion, one of the proteins was selected for further analysis. The thesis involves optimization of its expression and purification as well as its detailed biophysical characterization. (In Czech) Key words: protein evolution, protein structure, random sequences Detailed record

See also: similar author names
2	Simonová, Jaroslava
3	Simonová, Jasna
3	Šimonová, Jana
2	Šimonová, Jindra
3	Šimonová, Jitka

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