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National Repository of Grey Literature

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Escherichia coli O83:K24:H31 – potenciální živý nosič pro mukozální vakcíny Šimšová, Marcela ; Schwarzer, Martin ; Hrnčíř, Tomáš ; Hudcovic, Tomáš ; Kozáková, Hana ; Šebo, Peter The aim of our study was to create a mucosal vaccine for impoverished population. We used Escherichia coli O83:K24:H31 as live vector. It produced fused antigen M. tuberculosis fused antigens Ag85B-Esat6 (H1) which was inserted into a surface-exposed loop of the outer membrane protein LamB. Mice were given the bacteria according to the immunization protocol, they received free soluble H1 antigen with the mucosal adjuvant LTK63 as intranasal boost. We observed increase of total IgA on the intestine mucosa after colonization of E. coli O83 and induction of specific anti-H1 IgA level in small intestine Detailed record

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3	Šimsová, Marie
6	Šimsová, Martina

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