|
|
Determination of fluoride in toothpaste using SIA system
Barvínková, Zuzana ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Zuzana Barvínková Consultant: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Thesis: Determination of fluoride in toothpaste using SIA system Determination of fluorides was tested in the toothpastes using the SIA system. For determination of fluorides reaction of aluminium ions with aluminon that forms a red complex was used. After addition of a fluoride solution, aluminium ions were released from this complex. Spectrophotometry was used for the detection. The calibration range for sodium fluoride was linear over the range of 3x10-3 to 1x10-2 mol.l-1 . The repeatabitily of the measurement was tested for blank solution (water) and for the solutions of sodium fluoride 5x10-3 mol.l-1 and 6.25x10-3 mol.l-1 . The repeatability was in the range of 1.19 - 3.70 %, that was found acceptable. Then, potencially interfering ions were identified, which may effect determination of fluorides. Following the composition of usual toothpastes interferents could be: Cl- , Br- , I- , Ca2+ , PO4 3- , Zn2+ , CO3 2- , Al3+ , CH3COO- , Sn2+ , tartrate anion, citrate anion, lauryl sulphate anion, sorbitol and methylparaben. Among interfering ions, which influence the determination in low concentration, belong: Ca2+ ,...
|
|
|
Development and applications of affinity carrier for isolation of human carbonyl-reducing enzymes
Andrýs, Rudolf ; Wsól, Vladimír (advisor) ; Šebela, Marek (referee) ; Šatínský, Dalibor (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Rudolf Andrýs Supervisor: Prof. Ing. Vladimír Wsól, Ph.D Title of dissertation thesis: DEVELOPMENT AND APPLICATION OF AFFINITY CARRIER FOR ISOLATION OF HUMAN CARBONYL-REDUCING ENZYMES For several millennia the human medicine is based on application of small bioactive molecules that are administered in the form of plant extracts or synthetic compounds. However, their use in modern medicine is not possible without a detailed understanding of their biochemical effects and identification of their molecular targets. Chemical proteomics based on the specific recognition between the bioactive molecule and the target molecule is currently the most widely used techniques for identification of molecular targets of small molecules. Compared to conventional biochemical methods (e.g. 2D electrophoresis), chemical proteomics represents particularly sensitive and very selective technique that enable successful identification of biomolecules from complex biological samples that are naturally presented in very small concentrations. Carbonyl- reducing enzymes, which play an important role in physiology due to their involvement in metabolism of various endogenous (e.g. prostaglandins, steroid...
|
|
|
Development and validation of HPLC method for determination of retinyl esters in human serum using monolithic columns.
Krčmová, Lenka ; Solich, Petr (advisor) ; Šatínský, Dalibor (referee)
DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR DETERMINATION OF RETINYL ESTERS IN HUMAN SERUM USING MONOLITHIC COLUMN Absorption test of vitamin A is used for monitoring of intestinal permeability and may represent a sensitive indicator of intestinal damage. In this study, a simple and rapid reversed- phase high-performance liquid chromatography (RP-HPLC) procedure for selective and sensitive determination of retinol, -tocopherol, retinyl-palmitate and retinyl-stearate in blood serum has been developed and used for the monitoring of intestinal mucosal damage in cancer patients treated with cytotoxic drugs after absorption test of vitamin A. The HPLC instrumentation Series 200 LC from Perkin Elmer (Norwalk, USA) with diode- array detector (DAD) was used for the analysis. Separation of retinol, -tocopherol, retinyl- palmitate and retinyl-stearate were performed using the monolithic column Chromolith Performance RP-18e, 100 x 4.6 mm (Merck, Darmstadt, Germany). The gradient elution was used at the flow rate 3 ml min-1 ; mobile phase methanol:water (95:5) in 0-2.1 min and methanol:2-propanol (60:40) in 2.1-4.9 min. The total time of analysis was 6 min. The injection volume of sample was 20 μl and analysis was done at ambient temperature. The detection of retinol, -tocopherol and retinyl esters was carried out...
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
HPLC method development and validation for analysis of anthocyanins in highbush blueberries
Křížová, Kateřina ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kateřina Dostálová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: HPLC method development and validation for analysis of anthocyanins in highbush blueberries HPLC method for simultaneous determination of anthocyanins: delphinidin-3-O-galactoside, delphinidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O- arabinoside, peonidin-3-O-glucoside and malvidin-3-O-galactoside in 21 varieties of blueberries ('Spartan', 'Sunrise', 'Toro', 'Bluegold', 'Croatan', 'Northland', 'Duke', 'Gila', 'Jersey', 'Bluecrop', 'Herbert', 'Berkeley', 'Patriot', 'Darrow', 'Bluetta', 'Collins', 'Brigitta', 'November', 'Iranka', 'Bluejay' and 'Rancocas') was developed and optimized in this thesis. Kinetex PFP column, 150 x 4.6 mm (particle size 2.6 µm) was used for the analysis. The detection was performed by DAD detector at 520 nm, column temperature of 50řC using gradient elution with a mobile phase of acetonitrile/ 2 % formic acid at a flow rate of 1.0 ml/min. The total time of the analysis of one sample took 21 minutes. Keywords: HPLC, blueberries, anthocyanins, polyphenols, antioxidants, delphinidin-3-O- galactoside, delphinidin-3-O-glucoside,...
|
|
| |
guest ::
