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Non-library entities involved in information education of users of public libraries
Švejdová, Veronika ; Landová, Hana (advisor) ; Nekolová, Kateřina (referee)
The diploma thesis deals with non-library entities that collaborate with public libraries on the deepening of information literacy of library users. The theoretical part presents legislation and important documents that are connected with this topic. The following is an explanation of the terms that occur at work. The main part of the text is a research section that describes in detail used methods, a content analysis and a questionnaire survey, and their results. The conclusion provides selected examples of this cooperation in Czech and foreign public libraries.
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Preparation of monoclonal antibodies against Borrelia
ŠVEJDOVÁ, Veronika
In my bachelor thesis I deal with preparation of monoclonal antibodies against antigens of Borrelia. In theoretical section I describe genus Borrelia and antibodies. The introduction deals with the history of discovery of the spirochete that causes Lyme disease, vectors of this disease and classification of Borreliae according to geographical occurrence. Taxonomic categorization and description of the bacterium followers. It is focused on flagellum, arrangement of genetic material and on external surface antigens important for this bacterium. In the next section I describe Borrelia vector - host interactions and the development of immune response during the disease. Dermal symptoms of the Lyme disease are described in detail. A few words about relaxing fever are added. This chapter ends with description of laboratory diagnostics of Borrelia burgdorferi. Next, I focused on the characterization of antibodies and on preparation of monoclonal antibodies by hybridoma technology. I briefly describe ways of their utilization. Practical part of Bachelor thesis was carried out in the laboratories of Faculty of Science. Here, with the help of hybridoma technology, I fused myeloma cells with splenocytes of immunized mice producing hybridomas. These hybridomas I cultivated in medium, which I subsequently tested for occurrence of antibodies by ELISA. Hybridomas having high productivity were cloned, frozen and stored in liquid nitrogen. Finally I purified monoclonal antibodies, concentrated them and tested their titre.
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Application tasks of differential calculus of two variables
ŠVEJDOVÁ, Veronika
The aim of this work is to form the collection of answered riders of differencial calculus function with two variables. The work includes examples of determination of local function and solving of two-variable collocations. Examples will be arranged according difficulty and completed by graphical representation.
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