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Regulation of lipogenesis in human adipose tissue: Effect of metabolic stress, dietary intervention and aging.
Šrámková, Veronika ; Rossmeislová, Lenka (advisor) ; Cahová, Monika (referee) ; Kuda, Ondřej (referee) ; Tanti, Jean-Francois (referee)
Adipose tissue (AT) is a complex organ specialised in safe storage and release of energy as lipids. The adipose organ is therefore essential for the maintenance of energy homeostasis. The prototypical cells of AT are adipocytes, emerging from the precursors in a process called adipogenesis. Adipogenesis itself is tightly connected with lipogenesis, i.e. with the synthesis of fatty acids and triglycerides. Various stimuli can disturb adipocyte differentiation and lipogenesis and thus contribute to AT dysfunction and development of associated metabolic diseases. This thesis was endoplasmicfocused on the investigation of lipogenesis in the context of reticulum stress (ERS), calorie restriction and aging. In Project A, we showed that exposition of adipocytes to high acute ERS inhibits expression of lipogenic genes and glucose incorporation into lipids. Moreover, chronic exposure of preadipocytes to ERS impaired both, lipogenesis and adipogenesis. On the other hand, chronic low ERS had no apparent effect on lipogenesis in adipocytes. These effects of ERS could therefore contribute to the worsening of AT function seen in obesity. The capacity of AT to store lipids decreases in aging, possibly due to the accumulation of senescence cells or higher ERS. In Project B, we investigated lipogenic capacity of...
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Smlouva o dílo v praxi městského/obecního úřadu
Šrámková, Veronika
The main aim of the diploma thesis is, based on knowledge of the legislation regarding a contract for work, to propose recommendations for streamlining examined obligations. Economic and law weaknesses were identified through the analysis of the contracts provided by Municipal office in Adršpach and by Municipal office in Teplice nad Metu-jí. This is followed by proposals to eliminate these negative impacts for both sides of the contractual relationship. Within the work, a qualitative investigation has been done with using techniques of open and half-open interview.
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Nursing care of women with Hyperstimulatory Syndrome
ŠRÁMKOVÁ, Veronika
During the past few decades, the occurrence of couples unable to conceive in the natural way, whether by the fault of the man or the woman, has been on the increase. In those cases, help is provided by medical science, with assisted reproduction methods, which over the past decades have overcome the limits of clinical experiments and have become routine practice in the treatment of unvoluntarily childless couples or marriages. In view of the steadily growing efficiency of those methods assisted reproduction is an increasingly frequent indication, specifically in the form of in vitro fertilization. Another step was the introduction of infertility treatment by oocyte donation. Both these methods involve the stimulation of ovaries, which may lead to various complications, such as ovarian hyperstimulation syndrome provoked by excessive ovary stimulation. As a result, the capillaries become excessively permeable for the liquid, which penetrates into the enterocoele sac and causes various clinical and laboratory complications. Nursing care is indispensable for such women patients. Midwives should know about all potential complications of assisted reproduction, they should have an individual approach to the patients and show empathy for women in whom a complication has appeared, as such women are experiencing a psychically demanding situation and midwives are of great support to them during hospitalisation. The aims, hypotheses and answers were ascertained by a qualitative survey carried out by non-standardized interviews of women who underwent ovarian hyperstimulation, and by a quantitative survey based on anonymous questionnaires distributed among midwives. All the aims, hypotheses and questions I set myself have been confirmed. My work could be used as information material for women undergoing artificial fertilization, for midwives working with those women and for students who would like to concern themselves with this issue.
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