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Study of expression and maturation of mitochondrial oxidative phosphorylation system during mammal's prenatal period
Mrhálková, Andrea ; Hůlková, Martina (advisor) ; Ješina, Pavel (referee)
Postnatal adaptation of neonate to extrauterine life is among others dependent on maturation of mitochondrial oxidative phosphorylation system (OXPHOS). It depends on effective mitochondrial biogenesis during fetal developement. The inadequate capacity of mitochondrial OXPHOS system plays an important role in the neonatal mortality and morbidity. Therefore the study of mitochondrial biogenesis on molecular and biochemical level is important to improve the care of very premature neonates, especially critically ill premature neonates. This thesis has been worked out in The laboratory for study of mitochondrial disorders (Department of Pediatrics, 1st Faculty of Medicine, Charles University in Prague). The thesis is based on molecular genetic analyses, which are focused on characterisation of ATP synthase gene expression and on changes in mitochondrial DNA content during human and rat fetal development. The results provide the better insight into mitochondrial respectively ATP synthase biogenesis during human and rat fetal development.
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The study of mitochondrial biogenesis during fetal development
Hůlková, Martina
Postnatal adaptation of neonate to extrauterine life is among others dependent on effective mitochondrial biogenesis during fetal development. Therefore the study of mitochondrial biogenesis on molecular and biochemical level may improve the care of very premature neonates, especially critically ill premature neonates. This thesis has been worked out in The laboratory for study of mitochondrial disorders (Department of Pediatrics, 1st faculty of Medicine, Charles University in Prague). The thesis is based on molecular genetic analyses, which are focus on characterization of expression of gene involved in mitochondrial biogenesis including gene of oxidative phosphorylation system (OXPHOS) and on changes in mtDNA content during human fetal development. The results than enable : Efective analysis of the mRNA expression level by quantitative real- time PCR method in fetal tissues. Analysis of the changes in the mtDNA content in different fetal tissues To understand and to explain the tissue-specific differences in expression of the OXPHOS genes and of the genes involved in mtDNA transcription and in regulation of mtDNA content during second trimester of gestation.
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Painter and collector Miloš Malina and his bequest to Středočeské muzeum in Roztoky
Hůlková, Martina ; Pech, Milan (advisor) ; Czumalo, Vladimír (referee)
The Bachelor's thesis introduces a less-known artist and collector Miloš Malina, who is exceptional for combining both his passions in his artistic creations. The work deals mainly with the reference stored in the Central Bohemian Museum, where is his entire heritage, which Malina has left to the Museum still during his lifetime. The thesis is divided into three parts, which present the artist himself and his estate. At the end of the thesis I focus on Malina's work. The first part presents his life, studies and employment. It is also concerned with his ambiguous artistic style, which was influenced by some friends artists as Filla (Malina was inspired by his Cubist paintings), Špála (the same preoccupation with the blue colour), even foreign artists as Cézanne (use of apples in his still-lifes). Women, who surrounded him his whole life, were a great inspiration. I focused on the why he selected some of them and what about them was interesting for him. In the second part I will show how the whole legacy got into the Central Bohemian Museum. I will also mention how the legacy was thematically divided and stored in different individual depositories. In conclusion I not only focused on his work. I also aimed to the way his work reflects his fervour for collecting.
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The study of mitochondrial biogenesis during fetal development
Hůlková, Martina
Postnatal adaptation of neonate to extrauterine life is among others dependent on effective mitochondrial biogenesis during fetal development. Therefore the study of mitochondrial biogenesis on molecular and biochemical level may improve the care of very premature neonates, especially critically ill premature neonates. This thesis has been worked out in The laboratory for study of mitochondrial disorders (Department of Pediatrics, 1st faculty of Medicine, Charles University in Prague). The thesis is based on molecular genetic analyses, which are focus on characterization of expression of gene involved in mitochondrial biogenesis including gene of oxidative phosphorylation system (OXPHOS) and on changes in mtDNA content during human fetal development. The results than enable : Efective analysis of the mRNA expression level by quantitative real- time PCR method in fetal tissues. Analysis of the changes in the mtDNA content in different fetal tissues To understand and to explain the tissue-specific differences in expression of the OXPHOS genes and of the genes involved in mtDNA transcription and in regulation of mtDNA content during second trimester of gestation.
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Study of expression and maturation of mitochondrial oxidative phosphorylation system during mammal's prenatal period
Mrhálková, Andrea ; Hůlková, Martina (advisor) ; Ješina, Pavel (referee)
Postnatal adaptation of neonate to extrauterine life is among others dependent on maturation of mitochondrial oxidative phosphorylation system (OXPHOS). It depends on effective mitochondrial biogenesis during fetal developement. The inadequate capacity of mitochondrial OXPHOS system plays an important role in the neonatal mortality and morbidity. Therefore the study of mitochondrial biogenesis on molecular and biochemical level is important to improve the care of very premature neonates, especially critically ill premature neonates. This thesis has been worked out in The laboratory for study of mitochondrial disorders (Department of Pediatrics, 1st Faculty of Medicine, Charles University in Prague). The thesis is based on molecular genetic analyses, which are focused on characterisation of ATP synthase gene expression and on changes in mitochondrial DNA content during human and rat fetal development. The results provide the better insight into mitochondrial respectively ATP synthase biogenesis during human and rat fetal development.
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