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Preparation and characterization of nanodiamonds modified with biocompatible polymers
Kvaková, Klaudia ; Skoumalová, Petra (oponent) ; Cígler, Petr (vedoucí práce)
Nanodiamonds (NDs, 250 nm) were oxidized, separated by size and the polyglycerol layer was polymerized on the ND's surface. The polymerization was achieved by acid catalyzed ring opening multi-branching polymerization of glycidol with the hydroxyl and carboxylic groups formed, during oxidation on the NDs surface. The polyglycerol layer increases the stability and biocompatibility of the NDs. After the coating, a terminal propargyl group was introduced by glycidyl propargyl ether addition. The presence of the alkyne group enables further modification using copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC-click). Employing CuAAC click reaction, a mannose (with azide linker) was attached to the NDs surface. Thus prepared NPs were characterised from the physicochemical point of view. The size distribution was measured by dynamic light scattering (DLS), and it was further confirmed by transmission electron microscopy (TEM). Inertness towards biological conditions was evaluated (stability tests in 1xPBS, 10xPBS and 100 % FBS) and zeta potential was measured. Next, fluorescent nanodiamonds (FNDs, 250 nm) with attached D-mannose were prepared. According to the thermogravimetry results, DLS measurement and TEM images, it was determined, that NDs are covered by thin polyglycerol layer. Presence of glycidol coating was further confirmed by stability tests. Presence of attached D-mannose was supported by Fourier-transform infrared spectroscopy (FTIR). Prepared polyglycerol coated NDs are well suited for in vitro and in vivo testing. That was proven by their stability in high salt concentration media such as phosphate buffer saline (PBS) and low protein binding properties in fetal bovine serum (FBS).
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Preparation and characterization of nanodiamonds modified with biocompatible polymers
Kvaková, Klaudia ; Skoumalová, Petra (oponent) ; Cígler, Petr (vedoucí práce)
Nanodiamonds (NDs, 250 nm) were oxidized, separated by size and the polyglycerol layer was polymerized on the ND's surface. The polymerization was achieved by acid catalyzed ring opening multi-branching polymerization of glycidol with the hydroxyl and carboxylic groups formed, during oxidation on the NDs surface. The polyglycerol layer increases the stability and biocompatibility of the NDs. After the coating, a terminal propargyl group was introduced by glycidyl propargyl ether addition. The presence of the alkyne group enables further modification using copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC-click). Employing CuAAC click reaction, a mannose (with azide linker) was attached to the NDs surface. Thus prepared NPs were characterised from the physicochemical point of view. The size distribution was measured by dynamic light scattering (DLS), and it was further confirmed by transmission electron microscopy (TEM). Inertness towards biological conditions was evaluated (stability tests in 1xPBS, 10xPBS and 100 % FBS) and zeta potential was measured. Next, fluorescent nanodiamonds (FNDs, 250 nm) with attached D-mannose were prepared. According to the thermogravimetry results, DLS measurement and TEM images, it was determined, that NDs are covered by thin polyglycerol layer. Presence of glycidol coating was further confirmed by stability tests. Presence of attached D-mannose was supported by Fourier-transform infrared spectroscopy (FTIR). Prepared polyglycerol coated NDs are well suited for in vitro and in vivo testing. That was proven by their stability in high salt concentration media such as phosphate buffer saline (PBS) and low protein binding properties in fetal bovine serum (FBS).
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