Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Porovnání promotorů pro využití v klíštěcím (\kur{Ixodes}) expresním systému
MUSTACOVÁ, Johana
Gene manipulation can be a convenient tool for tick control, however functional procedure for gene manipulation of ticks was not determined yet. The creation of plasmid vectors with high ability of gene expression driven by functional promoters is crucial for genetically modified ticks. To investigate effective tools for tick genes manipulation within the scope of this master thesis, transfection procedures for various types of Ixodes tick cell lines were optimized. As well as expression vectors for use in tick cells were tested. For this purpose, expression plasmid vectors containing luciferase reporter genes driven by eukaryotic and viral promoters were used.
Interakce \kur{Borrelia burgdorferi} s. s. a \kur{Borrelia afzelii} s buněčnými liniemi klíšťat \kur{Ixodes ricinus} a \kur{Ixodes scapularis}
DOSTÁLOVÁ, Karolína
Borrelia burgdorferi sensu lato is a complex of pathogenic spirochetes causing a serious, multi-organ infection, Lyme disease. Lyme disease is mainly transmitted to humans by infected ticks of the genus Ixodes. Currently, there are more than 20 Borrelia species known. Borrelia is an extracellular pathogen and expresses a high number of immunogenic outer membrane proteins necessary for infection and maintenance within the tick and the host. The thesis deals with methods used for investigating the interaction of Borrelia with tick cell lines.

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