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Isolation and fractionation methods for determination of humic substances content in South Moravian lignite
Černý, Pavol ; Kalina, Michal (oponent) ; Enev, Vojtěch (vedoucí práce)
This bachelor thesis focuses on isolation and fractionation of humic substances (HS) from the natural matrix of lignite, which comes from southern Moravia from the mine Mír Mikulčice. In the theoretical part, it informs the reader about the issue, starting with information about lignite and HS. In this work, the standard procedure of isolation and fractionation of HS was used as according to the International Humic Substances Society (IHSS). The content of HS in the natural matrix of lignite was determined, and then isolated HS were subjected to physicochemical characterization – elemental analysis (EA), thermogravimetric analysis (TGA), UV/Vis spectrometry, infrared spectrometry with Fourier transformation (FTIR) and the acidity of humic acids (HA) was determined by potentiometric titration. EA was used to detemine the content of biogenic elements, moisture and ash content were determined by TGA. By using FTIR, various functional groups and building blocks that are present in supramolecules of HS were indentified and absorption coefficients EET/EBT, E2/E4, E2/E3 a E4/E6 were determined by UV/Vis spectrometry. Results represent good characterization of HS isolated from lignite and are in agreement with scientific literature.
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Isolation and fractionation methods for determination of humic substances content in South Moravian lignite
Černý, Pavol ; Kalina, Michal (oponent) ; Enev, Vojtěch (vedoucí práce)
This bachelor thesis focuses on isolation and fractionation of humic substances (HS) from the natural matrix of lignite, which comes from southern Moravia from the mine Mír Mikulčice. In the theoretical part, it informs the reader about the issue, starting with information about lignite and HS. In this work, the standard procedure of isolation and fractionation of HS was used as according to the International Humic Substances Society (IHSS). The content of HS in the natural matrix of lignite was determined, and then isolated HS were subjected to physicochemical characterization – elemental analysis (EA), thermogravimetric analysis (TGA), UV/Vis spectrometry, infrared spectrometry with Fourier transformation (FTIR) and the acidity of humic acids (HA) was determined by potentiometric titration. EA was used to detemine the content of biogenic elements, moisture and ash content were determined by TGA. By using FTIR, various functional groups and building blocks that are present in supramolecules of HS were indentified and absorption coefficients EET/EBT, E2/E4, E2/E3 a E4/E6 were determined by UV/Vis spectrometry. Results represent good characterization of HS isolated from lignite and are in agreement with scientific literature.
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