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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.01 vteřin. 
Deformation response of polydimethylsiloxane substrates subjected to uniaxial quasi-static loading
 Vinařský, V. ; Martino, F. ; Forte, G. ; Šleichrt, Jan ; Rada, Václav ; Kytýř, Daniel
To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. PDMS based material is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore, we adapted recently available protocol aiming to prepare substrates which offer stiffness in physiological range 5−100 kPa using various mixtures of Sylgard. An in-house developer loading device with single micron position tracking accuracy and sub-micron position sensitivity was adapted for this experimental campaign. All batches of the samples were subjected to uniaxial loading. During quasi-static experiment the samples were compressed to minimally 40% deformation. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated.
Úplný záznam
Instrumentation of four-point bending test during 4D computed tomography
 Kytýř, Daniel ; Fíla, Tomáš ; Koudelka_ml., Petr ; Kumpová, Ivana ; Vopálenský, Michal ; Vavro, Leona ; Vavro, Martin
High-resolution time-lapse micro-focus X-ray computed tomography is an effective method for investigation of deformation processes on volumetric basis including fracture propagation characteristics of non-homogeneous materials subjected to mechanical loading. This experimental method requires implementation of specifically designed loading devices to X-ray imaging setups. In case of bending tests, our background research showed that no commercial solution allowing for reliable investigation of so called fracture process zone in quasi-brittle materials is currently available. Thus, this paper is focused on description of recently developed in-situ four-point bending loading device and its instrumentation for testing of quasi-brittle materials. Proof of concept together with the pilot experiments were successfully performed in a CT scanner TORATOM. Based on results of the pilot experiments, we demonstrate that crack development and propagation in a quasi-brittle material can be successfully observed in 3D using high resolution 4D micro-CT under loading.
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