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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.00 vteřin. 
Fotostabilization of biopolymeric materials for regenerative medicine
 Izsák, Dávid ; Muchová, Johana (oponent) ; Brtníková, Jana (vedoucí práce)
Non-stabilized biopolymers often show poor mechanical properties. The stabilization and improvement of these properties could be accomplished by crosslinking, but the traditionally used crosslinking agents are rather cytotoxic and harmful for the human body. The aim of this work is to find a suitable non-toxic crosslinking method that would be efficient enough, but at the same time would not represent danger to any organism. The crosslinking method developed in the presented bachelor thesis should be comparatively efficient as traditionally used methods (e.g. chemical carbodiimide-based crosslinking) as well as potentially less cytotoxic by use of naturally derived substances. The proposed alternative crosslinking method should improve the strength and stability of the crosslinked biopolymers and enhance their biological functionality. Specifically, the presented work deals with the stabilization of collagen scaffolds using vitamin important for human metabolism followed by short- and long-wavelength irradiation. The theoretical part contains a general overview of possible non-toxic crosslinking agents, describes the structure of collagen and characterizes its properties and with the properties of used vitamin as a crosslinking agent. The experimental part deals with the preparation and crosslinking optimization of collagen scaffolds. Swelling, degradation and morphology of scaffolds as well as collagen secondary structure were evaluated. As a result, sufficiently strong crosslinked scaffolds were obtained.
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Micro CT 3D visualization and analysis of collagen scaffolds
 Jakubíček, R. ; Chmelík J.
In this paper we describe proposed methodology of image processing of collagen scaffolds. The description and visualization of the 3D porous structure is essential for tissue regeneration. First part of work deals with delimitation of fibrillar and cellular structure of collagen using local adaptive cluster analysis with fuzzy logic. In next section the segmentation of pores and subsequent analysis of structure features is reported. Eventually, we illustrate and discuss achieved results.
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