Národní úložiště šedé literatury Nalezeno 3 záznamů.  Hledání trvalo 0.01 vteřin. 
The role of Q tRNA modification in codon biased translation in \kur{Leishmania mexicana}
ŠVÁBOVÁ, Monika
The aim of the thesis was to standardize polysome profiling and ribosome profiling protocols for the parasitic protist Leishmania mexicana in order to identify the effect of Q-tRNAs in translation. In addition, we analyzed the role of queuosine (Q) t-RNA modification in the cell cycle of L. mexicana. The proteomics analysis was carried out in order to elucidate the role of Q-tRNAs in protein folding.
Role of queuosine tRNA modification in the parasitic protist \kur{Trypanosoma brucei}
KULKARNI, Sneha Sunil
This study analyzes the queuosine (Q) tRNA modification and the enzyme responsible for Q formation, the tRNA guanine transglycosylase (TGT), in the parasitic protist \kur{Trypanosoma brucei}. We describe the interdependence of Q modification and tRNA subcellular trafficking, and how these two factors regulate codon biased translation. The effect of Q on translation has serious implications in the virulence of the parasite inside the mammalian host.
Elucidating the subunit composition of tRNA-guanine transglycosylase in \kur{Trypanosoma brucei}
BĚHÁLKOVÁ, Veronika
The aim of this thesis was to elucidate the relationship and/or interaction between two tRNA-guanine transglycosylase paralogs (putative subunits of the TGT enzyme) identified in Trypanosoma brucei. The overexpression of these proteins was tested in a rescue experiment in a Schizosaccharomyces pombe experimental system.

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