|
|
Cell transplantation methods in cardiology
Kukhta, Dziyana ; Hežová,, Reneta (oponent) ; Skopalík, Josef (vedoucí práce)
This master’s thesis is devoted to tissue engineering, notably to creation homogeneous, isotropic and planar cardiac muscle cell layer using two technologies scaffold-based and scaffold-free. Firstly it describes the histology of heart wall, cardiac muscle cells, and cells cultures. This is followed by a description of tissue engineering, which includes cell sheet technology and scaffold-based tissue engineering. And at the end of the theoretical part application of tissue engineering and cell visualisation assay are described. The practical part is dedicated to the creation of a planar cell layer from cardiomyocytes and fibroblasts, using information from the theoretical part.
|
|
|
Simulation of adhesion and transmigration imune-cells through capillary wall
Morgaenko, Katsiarina ; Čmiel, Vratislav (oponent) ; Chmelíková, Larisa (vedoucí práce)
Theoretical part of this study includes biophysical description of endothelial layer and cell transmigration through it. There is descripted characteristics of all important components used for creation of capillary model in vitro (endothelial cells, stem cells, white blood cells, Calcein AM, PKH26). Practical part of the study devotes to fabrication of two-dimensional and three-dimensional in-vitro models of endothelial layers, processing of visualization by microscope and testing interaction between leukocytes, stem cells and endothelial layer. In conclusion is proposed the best form and size of microfluidic models simulated capillary.
|
|
|
Cell transplantation methods in cardiology
Kukhta, Dziyana ; Hežová,, Reneta (oponent) ; Skopalík, Josef (vedoucí práce)
This master’s thesis is devoted to tissue engineering, notably to creation homogeneous, isotropic and planar cardiac muscle cell layer using two technologies scaffold-based and scaffold-free. Firstly it describes the histology of heart wall, cardiac muscle cells, and cells cultures. This is followed by a description of tissue engineering, which includes cell sheet technology and scaffold-based tissue engineering. And at the end of the theoretical part application of tissue engineering and cell visualisation assay are described. The practical part is dedicated to the creation of a planar cell layer from cardiomyocytes and fibroblasts, using information from the theoretical part.
|
|
|
Simulation of adhesion and transmigration imune-cells through capillary wall
Morgaenko, Katsiarina ; Čmiel, Vratislav (oponent) ; Chmelíková, Larisa (vedoucí práce)
Theoretical part of this study includes biophysical description of endothelial layer and cell transmigration through it. There is descripted characteristics of all important components used for creation of capillary model in vitro (endothelial cells, stem cells, white blood cells, Calcein AM, PKH26). Practical part of the study devotes to fabrication of two-dimensional and three-dimensional in-vitro models of endothelial layers, processing of visualization by microscope and testing interaction between leukocytes, stem cells and endothelial layer. In conclusion is proposed the best form and size of microfluidic models simulated capillary.
|
host ::
RSS.