Národní úložiště šedé literatury Nalezeno 27 záznamů.  začátekpředchozí18 - 27  přejít na záznam: Hledání trvalo 0.00 vteřin. 
Very low energy STEM for biology
Frank, Luděk ; Nebesářová, Jana ; Vancová, Marie ; Paták, Aleš ; Müllerová, Ilona
Examination of tissue sections with transmitted electrons has been performed at energies of hundreds and tens of eV with thicknesses of sections of 10 nm or less. This was possible by employing the cathode lens principle working without lowest energy limitations with the help of biasing the sample to a high negative potential. The reflected and transmitted electrons were attracted with the same electric field to earthed detectors situated above and below the sample. Very high image contrasts have been obtained even for samples free of any heavy metal salts for contrast enhancement.
První zkušenosti s nízkonapěťovým elektronovým mikroskopem
Nebesářová, Jana
Je krátce popsán nízkonapěťový elektronový mikroskop LV EM 5 vyráběný firmou Delong Instruments a jsou prezentovány první zkušenosti s přípravou biologických preparátů pro pozorování v tomto mikroskopu. Je to unikátní přístroj, který je schopen v lékařském a biologickém výzkumu nahradit vysokonapěťový TEM.
Observation of biological material in low voltage transmission electron microscope
Vancová, Marie ; Nebesářová, Jana
Different methods of specimen preparation for low voltage transmission electron microscopy (LVEM) were evaluated. The contrast of the image in LVEM, contrary to high voltage TEM, is strongly influenced by the thickness of the specimen. The resin embedding media used and the process of sectioning employed contributed to image quality and resolution.
Comparison of the cell structure of tissues prepared by different methods for transmission electron microscopy
Vancová, Marie ; Nebesářová, Jana
The aim of this work is to show how methods which are able to retain antigenicity of tissues can influence the structural appearance of the specimen. We compared the ultrastructure of tissues conventionally embedded in hydrophobic epoxides, with tissues embedded in hydrophilic acrylic resins (polymerized by heat or UV light) and cryo-sections.
The first experience with the specimen preparation for low-voltage transmission electron microscope
Nebesářová, Jana ; Vancová, Marie
The low-voltage transmission electron microscope, working in the TEM mode, requires ultrathin sections with a thickness 20-30nm. This demand brings problems with a section cohesion caused by insufficient resin infiltration and with a chatter caused by unequal thickness of the section. The contrast of unstained biological specimens in LV TEM is comparable with the contrast obtained in HV TEM of stained specimens.
Electron microscopical study of embryonic envelopes in the tapeworm Proteocephalus torulosus (Cestoda: Proteocephalidea)
Bruňanská, Magdaléna ; Nebesářová, Jana ; Scholz, Tomáš
The ultrastructure of the embryonic envelopes of the cestode Proteocephalus torulosus (Batsch, 1786), a parasite of cyprinid fish, was examined by transmission electron microscopy.
The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus
Slavíčková, K. ; Berger, Josef ; Nebesářová, Jana ; Němec, Václav
The importance of fixation procedures for transmission electron microscopy of haemocytes of Pyrrhocoris apterus.

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