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Anti-inflammatory and immunomodulatory activities of Ixodes ricinus salivary proteins
CHLASTÁKOVÁ, Adéla
Ticks are highly specialized obligate ectoparasites whose life cycle is fully dependent on obtaining a blood meal from a host. To prevent blood clotting and counteract the host's immune and wound healing responses, ticks secrete saliva, containing hundreds to thousands of proteins and non-proteins, into the feeding cavity. This thesis focuses on deciphering the function of three serpins (inhibitors of serine proteases) expressed in the salivary glands of the European tick Ixodes ricinus. Iripin-1, Iripin-3, and Iripin-8 were produced in the Escherichia coli expression system, and their effects on the immune response were evaluated using various in vitro assays and a mouse model of acute inflammation. Furthermore, the thesis involves one review article that summarizes the role of serpins in tick physiology and tick-host interaction.
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Zavedení a optimalizace \kur{in vivo} modelů zánětu a jejich využití pro funkční analýzu inhibitorů proteáz z klíštěcích slin
CHLASTÁKOVÁ, Adéla
Two murine models of acute inflammation, namely thioglycollate-induced peritonitis and carrageenan-induced paw edema, were optimized using non-steroidal anti-inflammatory drug indomethacin and corticosteroid dexamethasone. During the optimization phase, the presence of neutrophils, monocytes, macrophages, eosinophils, B cells and T cells in the peritoneal cavity at various time points after injection of thioglycollate medium was assessed via multicolor flow cytometry. Moreover, two different thioglycollate media (suppliers BD and Sigma-Aldrich) were compared for their ability to induce an inflammatory response. The optimization of thioglycollate-induced peritonitis and carrageenan-induced paw edema was followed by the evaluation of the anti-inflammatory activity of Ixodes ricinus cystatins G1 and G9 in both mouse models.
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Vliv délky sání klíštěte \kur{Ixodes ricinus} na přenos spirochéty \kur{Borrelia afzelii}
CHLASTÁKOVÁ, Adéla
Lyme borreliosis is a multisystem infectious disease caused by tick-borne spirochetes belonging to the \kur{Borrelia burgdorferi} sensu lato complex. It is generally believed that if a tick is attached for less than 24 hours, infection is unlikely because it requires between 36 and 48 hours of attachment for \kur{Borrelia burgdorferi} to travel from within the tick into its saliva. Nevertheless, there is evidence that transmission to a host may even occur in the early phases of tick feeding. We investigated the dynamics of \kur{Borrelia afzelii} transmission by nymphal \kur{Ixodes ricinus} ticks with special respect to the first day of tick attachment.
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