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Microfluidic device for cell counting and electrical lysis
Václavek, Tomáš ; Křenková, Jana ; Foret, František
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
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A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana ; Haselberg, R. ; Somsen, G. W. ; Foret, František
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
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