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	Characterization of peptide proteinase inhibitors isolated from boar seminal plasma Jelínková, Petra ; Tichá, M. ; Jonáková, Věra Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers. Úplný záznam
	Protein Characterization of Boar Epididymal Fluid Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Úplný záznam
	Characterization of peptide proteinase inhibitors isolated from boar seminal plasma Jelínková, Petra ; Tichá, M. ; Jonáková, Věra Seminal plasma of different mammalian species contains various types of proteins. Proteinase inhibitors are important components of seminal plasma and were found in all tested species. In the present communication we have shown that serine proteinase inhibitors participate (under physiological conditions) in the formation of associated complexes with other proteins from boar seminal plasma. Úplný záznam
	Separation of human seminal plasma proteins by selected chromatographic and electrophoretic methods Kraus, Marek ; Tichá, M. ; Jonáková, Věra We concentrated on proteins related to boar spermadhesins, which have not been identified, yet in human seminal plasma. We studied the cross-reactivity of antibodies against AQN and AWN boar spermadhesins with human seminal proteins. Úplný záznam
	Proteins from boar prostate secretion: their isolation and characterization Maňásková, Pavla ; Ryšlavá, H. ; Tichá, M. ; Jonáková, Věra Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and II spermadhesins. Úplný záznam
	An approach for isolation of human seminal plasma proteins Kraus, Marek ; Tichá, M. ; Jonáková, Věra Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against boar seprmadhesing AQN and AWN was studied with human seminal proteins. Úplný záznam
	Properties of serine proteinase inhibitors from boar seminal plasma Jelínková, Petra ; Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Inhibitors of proteinases are important components of seminal plasma. Their biological function in seminal plasma is to protect protein components of seminal plasma and male and female reproductive tracts against proteolytic degradation. My work is concentrated on characterization of inihibitors found in seminal plasma. Úplný záznam
	Protein interactions involved in the formation of aggregated forms of boar seminal plasma proteins Maňásková, Pavla ; Balínová, P. ; Tichá, M. ; Jonáková, Věra Mutual interactions of isolated proteins were tested by means of gel chromatography. The results obtained confirmed an existence of interactions between spermadhesins of the AQN and AWN families as well as between proteins involved in the formation of heterodimer PSPI/PSPII. Úplný záznam
	Serine proteinase inhibitors isolated from boar seminal plasma Jelínková, Petra ; Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Serine proteinase inhibitors were found in boar seminal plasma. The work was concentrated on isolation and characterization of proteinase inhibitors and study of the existence of inhibitors in complexes with other proteins in seminal plasma. Úplný záznam
	Povrchové proteiny spermií a semenné plazmy ve fertilizaci Jonáková, Věra ; Maňásková, Pavla ; Jelínková, Petra ; Balínová, P. ; Tichá, M. Savčí fertilizace zahrnuje vysoce regulované biochemické děje. My jsme se zaměřili na studium interakce proteinů semenné plasmy se spermií a na vzájemné interakce proteinů ze semenné plasmy. Úplný záznam

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