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Application of mica based pigments
Štengl, Václav ; Kalendová, A. ; Trojan, M.
Properties of sandwich pigments based on mica coated with metal oxide layers are described. Various mica types were characterized and used for pilot plant scale synthesis of pigments. Paints containing this kind of pigments were formulated and tested anticorrosive coatings. Synthesis and characterization of some thermally stable pigments based on mixed metal oxides is also described.
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Nuclear Fuel Loading Patterns at VVER Reactor Based Nuclear Power Plants
Šajdler, Miroslav ; Vojáčková, Jitka (referee) ; Katovský, Karel (advisor)
The Master's thesis focuses on loading patterns of nuclear reactors VVER. It describes the whole process of fuel cycle, since production to storage or reprocessing. The author puts emphasise on the middle part of fuel cycle in Czech nuclear power plants - Dukovany and Temelín and he also explains which fuels are used in both power plants now and which were used in history. The thesis also contains a basic overview of approaches to loading patterns optimisation in foreign countries. The final part of the thesis discusses practical calculation of loading patterns in the Block III of Nuclear power plant Dukovany, by using optimisation programme Athena and Moby-Dick macrocode.
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Partial purification and characterization of polygalacturonases of Geotrichum candidum.
Jäger, Jakub ; Breierová, Emília (referee) ; Stratilová, Eva (advisor)
This work discusses the possibilities of using microbial degradation of grape pomace, main waste material from wine production, to preparate industrially important enzymes. The issue is focused on the production of pectolytic enzymes, particularly polygalacturonase, by Geotrichum candidum CCY 16-1-29 via solid state fermentation on grape pomace. The theoretical part of the bachelor thesis focuses on studying plant cells and saccharides from which the plant cell wall is made of, mainly pectin. Cell wall sacharides were used as a carbon source for solid state fermentation (SSF) and pectin as an inductor of pectolytic enzymes. This bachelor thesis also deals with the enzymatic degradation of cell wall polysacharides. The greatest attention is paid to degrade pectin and pectolytic enzyme function. Production of pectolytic enzymes is mentioned subsequently. The last chapter from the theoretical part is dedicated to technical use of pectolytic enzymes. In the experimental part of this work I deal with the partial purification and characterization of majority polygalacturonase produced on the seventh day of cultivation, when another increase of extracellular polygalacturonase activity occurred. The yield of cultivation was 43,5 mg of protein extract /100 g of grape pomace. The extract contained protein, and its activity was lyophilisate. Its specific activity was protein. The enzyme was produced in at least four forms differing in pH optimum (4,0; 4,4; 4,8; 5,2). The pH optimum for majority polygalacturonase was 4,8. Action pattern of this enzyme determined as the dependence of polymeric substrate viscosity decrease on its degradation showed that the enzyme is a typical polygalacturonase with random action pattern (EC 3.2.1.15).Value of Km reached indicating a high affinity for this substrate. The amino acid sequence "SNNVVSNVNILSSQVVNSDNGVR" obtained by mass spectrometry after SDS-PAGE and tryptic digestion, was identified as a stretch of primary structure of polygalacturonase of Ap2PG1 G. candidum based on the comparison with proteins from the Uniprot database. It shows the highest similarity with other polygalacturonases of G. candidum S31PG1, S31PG2 and G. klebahnii PSE3. On the basis of this similarity to enzymes produced by phytopathogenic strains of G. candidum and the fact that this enzyme was not produced only in the early stages of cultivation, it can be assumed, that the strain of G. candidum CCY 16-1-29 acted also as a phytopathogenic strain.
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Car wrecks from the view of economy, technical treatment and environmental point of view
Nováková, Tereza ; Charvátová,, Michaela (referee) ; Kizlink, Juraj (advisor)
The bachelor’s thesis is focused on treatment of car wrecks in terms of economy, material recovery and environment. The thesis is only theoretical and the information are supported by various sources. The thesis defines waste, car wreck and selected carwreck as legal concepts. It delas with legislative measures in car wreck treatment both in the Czech republic and in European Union. The thesis maps current trends of development in composition of vehicles in the Czech republic compared to global scale. The thesis describes material composition of car wrecks and the managment proces. Further states the most common ways how to dismantle the ELVs, methods of recycling and the overview of the latest findings in the area of reuse of waste generated during processing of car wrecks. The thesis handle with car wreck not only as a whole but also the processing of individul parts. In a separate chapter are described processing plants and collection points for ELVs. It also contains necessary information to log out ELV from central register. The last chapter deals witf the scrap.
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Stevioside and rebaudioside use in the food industry in the Czech Republic and the European Union
PRAYER, Radek
The main objective of this work was a search process can use steviolglycosides in foodstuffs within the Czech Republic and the European Union in the period since 2000, focusing on legislation and non-food use. Based on journal articles and available legislative provisions, it is clear that Stevia is a plant with great potential to replace at least part of the current sweeteners. Stevia can also be used in cosmetics or in medicine because it contains substances that are beneficial to health. Legislation currently in force in the European Union and the Czech Republic permitted in food use steviolglycosides in high purity. Using Stevia plants or parts thereof, as food is not allowed.
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Arboretum of native woody plants Týn nad Vltavou
ŠÍMA, Jaroslav
Thesis contains a design of the arboretum of native woody plants of Czech Republic, which should be placed in the area of the Natural History Museum Semenec in Tyn nad Vltavou. Its aim is to introduce diversity of Czech trees and shrubs species. The arboretum is arranged so as to present particular vegetation zones. This indicator appears in terms of popular scientific mission of the arboretum as the most obvious and comprehensible. Special sections of arboretum are devoted to the azonal communities including those that develops in terms of marginal hydric series. Created arboretum of native woody plants of Czech Republic will serve to general public (tourists, school groups, univerzity students etc.) in the implementation of science educational programs
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Study and characterization of the recessive resistance genes to Pea seed-borne mosaic virus (PSbMV)
Konečná, Eva
Pea seed-borne mosaic virus (PSbMV) belongs among the most frequent viral pathogens causing severe yield and seed quality losses in field pea and other legumes. These losses might be the most effectively prevented by use of resistant varieties. Resistance to the common P1 strain of PSbMV is conferred by a single recessive gene (eIF4E), localized on LG VI (sbm-1 locus), while the resistance to lentil L1 strain is localized on LGII at sbm-2 locus. Germplasm of 2803 pea accessions was screened for eIF4E intron 3 length polymorphism using two sets of primer combinations, resulting in the detection of four eIF4EA-B-C-S variants, whose distribution was geographically structured. In order to identify gene underlaying sbm-2 locus, we have analyzed variation in the candidate eIF(iso)4E gene sequences from selected pea lines, reported as donors of resistance. we analyzed expression of both resistant and sensitive eIF4E alleles in F1 and F2 heterozygous plants, sensitive and resistant homozygotes after PSbMV infection, by qRT-PCR together with evaluation of viral concentration.
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Identification and molecular characterization of the putative immunophilins (IMMs) in the oilseed rape pathogens Leptosphaeria maculans, Leptosphaeria biglobosa, and Plasmodiophora brassicae
Sandhu, Khushwant Singh ; Ryšánek, Pavel (advisor) ; Radovan, Radovan (referee)
Oilseed rape is largely infected by several phytopathogens and two most economical important diseases are blackleg caused by fungus species complex Leptosphaeria maculans and L. biglobosa and clubroot caused by protist P. brassicae. The sequenced genomes of these phytopathogens provide opportunity to uncover various aspects related to disease infection, host pathogen interactions, plant disease resistance, and evolution of pathogens. Considering these we focused on one of the most conserved family called immunophilins (IMMs) in these genomes. IMMs are comprised of three structurally unrelated sub-families including cyclophilins (CYPs), FK506-binding proteins (FKBPs), and parvulin-like proteins (PARs). We identified putative members of IMMs in each phytopathogen using bioinformatics approaches. We further characterized the IMMs based on domain architecture, subcellular localization, exon-intron organization, transcriptomic expression patterns, gene ontology terms, conserved motifs presents and evolutionary analyses. IMMs are performing several vital roles in plants, animals and fungi. However, in phytopathogens their roles are not well established except for cyclophilin that implicates in pathogenicity in some phytopathogens. Therefore, we exploited the role of cyclophilin in L. maculans and L. biglobosa using expression profiles and in P. brassicae using Magnaporthe oryzae cyclophilin deletion mutant. Overall, we concluded that the cyclophilin acts as a virulence determinant in our studied phytopathogens. However, delineating the precise role of other IMMs would also be imperative. Taken together, our findings for the first time shed light on the highly conserved IMM family in the oilseed rape pathogens.
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