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Aspects of reproduction biology of the moss \kur{Helodium blandowii}
BRADÁČOVÁ, Jitka
Regeneration from stem and branch fragments of the moss Helodium blandowii was examined in common garden and field experiments. The treatments included one cm long terminal stem fragments and branches of one cm and half cm length with and without the terminal and their regeneration was observed in course of 3 months in the common garden experiment and 15 months in the field. Dynamics of Helodium patches was observed in course of 2 years in permanent plots of the nature reserve Ruda. Genetic stucture of all recent Czech populations of Helodium blandowii was investigated and compared with a pilot survey of populations from Scandinavia and Lithuania using the microsatellite markes developed for this study.
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Genetic diversity of cattle in Czech Republic
ČERVINKA, Václav
The thesis deals with genetic diversity in the Czech Republic. Firstly domestication of livestock in general and then domestication of cattle is decribed. History of cattle in the Czech Republic, the importance of cattle breeding and last but not least the condition of cattle in the Czech Republic is also introduced. The thesis is also focused on biodiversity, threats to biodiversity and its conservation and the levels of diversity are also specified. Genetic markers,microsatellites, minisatellites, SNP, polymorphic structural genes and also mutations are described. The cattle breeds farmed in the Czech Republic are specified next. The National Programme, genetic resources and genetic resources in the Czech Republic are evaluated. Finally genetic resources of " česká červinka" and "český strakatý skot" are described.
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Aspects of reproduction biology of the moss \kur{Helodium blandowii}
BRADÁČOVÁ, Jitka
Regeneration from stem and branch fragments of the moss Helodium blandowii was examined in common garden and field experiments. The treatments included one cm long terminal stem fragments and branches of one cm and half cm length with and without the terminal and their regeneration was observed in course of 3 months in the common garden experiment and 15 months in the field. Dynamics of Helodium patches was observed in course of 2 years in permanent plots of the nature reserve Ruda. Genetic stucture of all recent Czech populations of Helodium blandowii was investigated and compared with a pilot survey of populations from Scandinavia and Lithuania using the microsatellite markes developed for this study.
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Identification of parentage in chamois (Rupicapra rupicapra) from NP Gran Paradiso
Poláková, Radka
We analysed 47 faecal samples of chamois (32 samples of kids and 15 samples of females). The remaining DNA samples comprised 28 tissue samples of males and other females. Tissue samples were stored in 96% ethanol and faecal samples were placed straight into silicagel. DNA from faecal and tissue samples was extracted using commercial kits. Parentage was determined using the likelihood-based approach in CERVUS 3.0 and these results were confirmed by the program ML-Relate. We found parentage in the case of seven males that sired ten kids.
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Population genetics of two endangered fritillary butterflies in the Moravian Carpathians
LEŠTINA, Dan
Annotation Populations of two sympatric large fritillary species, the High Brown Fritillary (Argynnis adippe) and the Niobe Fritillary (A. niobe), were studied using microsatellite markers to assess and compare potential population subdivision in a relatively preserved landscape of the Czech part of the Carpathians. The results are confronted with data obtained from a smaller-scale mark-release-recapture study, with species? known life histories and namely with their conservation status, all of which is also considered in explaining the obtained patterns of genetic diversity.
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Small terrestrial mammals along natural and anthropogenic landscape barriers
BOHDAL, Tomáš
The dissertation summarises the result of the study of small terrestrial mammals (Eulipotyphla, Rodentia) along natural (watercourses) and anthropogenic (road, highway) landscape barriers. It points to an interesting phenomenon of road drainage ditches as a transitional migration environment, further it assesses the level of locomotion activity, the rate of structure of subpopulations and the frequency of crossing watercourses in the case of selected rodent species. It contributes with its results to solving problems concerning the effect of these landscape structures on the species diversity, ground mobility or genetic structure of small terrestrial mammals. ˙˙˙˙
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Breeding of genetic resource Bohemian Red cattle at the University Agricultural Enterprise, SBU in České Budějovice
ŤOUPALOVÁ, Michaela
At the turn of the 19th century a Czech red cattle, the breed, which was in our conditions bred a long time ago, was crossbreeding with a simen{\crq}s bull. Czech red cattle were gradually decreasing until now, when it is at the critical limit. At the 1991, in Department of Genetics, Breeding and Nutrition, at Faculty of Agriculture, University of South Bohemia, has begun regeneration of this rare, for our conditions original, breed. The Process of Bulls absorptive breeding of Czech red cattle has begun. Czech red cattle have by now more then 162 pcs. There are kept 43 head of cattle in the case of breeding under warranty ČZU Prague. JČU in Č.Budějovice has got 119 individuals under its guarantee, of which 60 is kept on the school farm Haklovy Dvory. At research was analyses data about growth and development of calves breeding without market production of milk and Data of cow milk efficiency breeding for milk production at stable manner. The highest milk yield was achieved at 173 days lactation. The Total quantity was 1 469 kg of milk. The highest average content of milk fat was 3,80 %, and content of milk protein was 3,52 %. Index of growth and development was calculated on age 120 {--} 210 days. Average weight of 4 cows at the age of 120 days was 114kg. The highest increase of weight was noticed at a bull {--} calf and was 934 g. Average weights of 2 pcs. on unified age 210 days was 221kg. The highest growth was achieved at a heifer with its 1 038g. Meanwhile analysis of genetic structure of population has begun and on the basis of results was characterized number of locusts and microsatellites at given population. Genotyping of locusts for DGAT, GH, b {--} LG, PRL, IGF BP3, PIT1, BLAD, LEP, DUMPS, b {--} CN, kappa casein, beta casein und ALFA S1 casein was performed in the set of 272 animals. Frequency was observed in microsatellites RM 012, BOVCASK 35, BOVIRP, BTO BCAM, BOVPAI, BOVSEMRN, SRC 97, IGF BP3, CSS 004, IDVG A - 9, BM 6117, BM 148, BM 4621,BM 6438, BM 2113, BM 1824, BPA, BMS1658, INRA 107, INRA 23, ETH 3, ETH 225, ETH 10, TGLA 122, TGLA 126 and TGLA 227.
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Genetic diversity of grayling (Thymallus thymallus L.) populations in the Czech Republic inferred from microsatellite markers
Papoušek, Ivo ; Halačka, Karel ; Kohout, Jan ; Šlechta, Vlastimil ; Vetešník, Lukáš ; Mendel, Jan
The grayling is among the most valuable fish species in salmonid waters of the Czech Republic. Genetic diversity of twelve grayling populations from all three watersheds of the Czech Republic has been assessed by two multiplex analyses of ten microsatellite loci. Allele frequencies and tests of genotype frequencies for deviation from Hardy-Weinberg equilibrium were carried out. Relationships between respective populations were assessed by Factorial Correspondence Analysis and Principal Coordinate Analysis. Analysis of multilocus microsatellite genotypes from three watersheds of the Czech Republic has shown that significant variability is present in Czech grayling populations. However, this variability has no geographical structure; individuals from all three watersheds form no distinctive groups. It seems that the original genetic structure of Czech grayling populations has already been lost as they have been uniformized by artificial management.
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