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Methods of assesment and treatment of coeliac disease
Valkus, Martin ; Omelková, Jiřina (referee) ; Vránová, Dana (advisor)
This diploma thesis deals with methods of determining and treatment of coeliac disease. In the theoretical part of the work sums up informations about coeliac disease - history, prevalence, etiopathogenesis, immunopathogenesis, possibilities of diagnosis, discusses about gluten-free diet, mentions legislation of the Czech Republic and the European Union and compares expensiveness of gluten-free diet and normal diet. The experimental part of this thesis compares PCR, ELISA and indirect immunofluorescence methods for assesment of determination of genetic predisposition and antibodies in coeliac disease (greatest emphasis was placed on the comparison of antibodies against gliadin and deamidated gliadin antibodies in IgA and IgG).
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Imunomagnetic separation of lactic acid bacteria using magnetic microparticles functionalised by antibodies
Vaňásek, Jakub ; Španová, Alena (referee) ; Trachtová, Štěpánka (advisor)
Immunomagnetic separation is based on binding of antibody with antigen, where antibody is bound to magnetic particle. In this thesis there were used particles of magnetic pearl cellulose with antiLactobacillus and antiBifidobacterium antibodies. Immunomagnetic separation method was optimalized and verified for its efficiency and specifity with bacterial and yeast cells. This cells were identified by polymerase chain reaction. Efficiency of immunomagnetic separation was verified on probiotic meat product, where Lactobacillus cells were isolated. With DNA from isolated Lactobacillus cells the high resolution melting was performed. The results show presence of several bacterial strains of Lactobacillus species.
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Tutorial in branch of internal medicine with a view to locomotive organs for education of radiological assistant
ZACHARDOVÁ, Martina
This work studies the availability of materials concerning the anatomy and disorders of the locomotive organs from the field of internal medicine. The result of studying these materials is an educational program, which is attached to this work on a CD. This program is to serve as an aid for teaching students attending the medical-social faculty and together with the Rheumatology Program offers the only education program focusing on disorders of the locomotive organs from the field of internal medicine; I found the Rheumatology Program on the Internet.
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