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Variabilita markeru TG5 a asociace k obsahu intramuskulárního tuku a marblingu u skotu
Grosová, Hana
The variability of the marker TG5 TG gene and its association with the intramuscular fat and marbling of meat in cattle was studied. Randomly selected population, composed of 237 individuals (bulls) of the Czech Pied cattle breed from four farms in the Czech Republic, waschosen for testing polymorphisms. Polymerase chain reaction was used to amplify a fragment of TG 545 bp in size. After amplification, the fragments digestion was performed using the restriction endonuclease BstYI. To verify the presence of the PCR product and to identify the sizes of digested frag-ments the horizontal agarose electrophoresis was performed. The population's absolute and relative frequencies of alleles and genotypes were calculated. The calculations reve-aled a high frequency of the T allele (25.98 %). In conclusion, statisticalanalysis was performed. As a result, the influence of C422T polymorphism in the 5'promoter region of the gene on the TG and on the IMT marblinghave not been proven, but on the contra-ry there was detected significant effect (p<0.05) of polymorphism content on the region of Lauric acid (C12:0). The results also reveal some evidence of polymorphism having influence on Timnodonic acid and meat pH.
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Slovak National Uprising
Orság, Michal ; Terén, Laco (referee) ; Ambrůz, Jan (advisor)
The aim of the thesis was to bring events after 1945, when there was a period of remembrance in the company , which served as a therapy , politics and aesthetics . Work on the memories gradually morphed into , among other things , to the monuments that act against collective oblivion . Arise place of remembrance , which relate to historical events and private stories . Partisan monuments fill in the country which they have to remind essentially absent . Again remembering you and gets to the surface again forgotten information that creating a logical connection form the structure . This structure is subject to change , which helped by rewriting and overlapping information in different layers of the structure . Neither art does not avoid the remembrance and forgetting , and creates a theme of a strategy , which is included rewriting . Media and individual override blank sheets in the past, currently , collecting and composing the mosaic , which allows you to create your own identity.
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