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National Repository of Grey Literature

333 records found  311 - 320  jump to record:

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	Photosynthetic electron transport in purple bacteria: An in vivo spectroscopic study BÍNA, David Electron transport in purple bacteria was studied using combination of absorption spectroscopy and induced bacteriochlorophyll fluorescence in whole cells in vivo. Focus is placed on relations between fluorescence yield, the state of the electron transport chain and the membrane potential. A laboratory-built absorption spectrophotometer-fluorimeter is described. Detailed record
	Effect of selected exogenous and endogenous factors on transformation efficiency SKALICKÝ, Tomáš The main goal of this bachalor thesis was to explore the possibility to use an ultrasound sonication as a way to increase the transformation efficiency of flax. Another part of this thesis is devoted to the searching for the seed sterilization method and finding an optimal concentration of kanamycin antibiotic useful for the selection of transformed plants. Detailed record
	Possibilities of the green fluorescent protein signal gene utilization for flax transformants identification and selection KŮTA, Václav An aim of the thesis was to verify possibilites of in vitro identification and selection of transformed flax tissues and plants based on the fluorescence assessment of objects transformed with a construct bearing the gfp gene coding for green fluorescent protein (GFP). Effect of plant genotype, explant type and modifications of the transformation protocol on fluorescence was evaluated too, as well as the possibility of use for selection of transgenic individuals. Detailed record
	On-line quantification of cyanobacteria in a raw water Novotná, Zlatica ; Maršálek, Blahoslav ; Trtílek, M. ; Rataj, T. A new fluorometer AOM (Algae Online Monitor) for the quantification of phytoplankton in raw water was tested on different localities. The AOM was serviceable in the variable field conditons. Because of low limit of detection and due the work in continuous mode with remote GSM data transfer to laboratory, the device is suitable for early warning system. Detailed record
	Potenciál kvantových teček při detekci jediné molekuly v buňkách a organelách Přikryl, Jan ; Klepárník, Karel ; Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Preisler, J. ; Foret, František This contribution is devoted to quantum dots usage for cell imaging in fluorescence microscopy. Fluorescence excitation by evanescence wave (caused by total internal reflection) is compared with conventional epifluorescence microscopy. Detailed record
	Syntéza modifikovaných fluorescenčních nukleosidů Riedl, Jan ; Hocek, Michal A series of modified nucleosides bearing the fluorescent substituted biaryl labels attached to nucleobase was prepared and their luminescent properties were evaluated. The modified nucleosides show strong fluorescence at ca. 400 nm when excited at 310 nm in water depending on substitution pattern. Detailed record
	Fluorescenční kinetická mikroskopie: 2D zobrazování variabilní fluorescence chlorofylu jednotlivých buněk řas se spektrálním rozlišením Prášil, Ondřej ; Šetlíková, Eva ; Veselka, D. The fluorescence kinetic microscopy (FKM) provides new dimension in application of chlorophyll fluorescence techniques. It combines classical fluorescence microscopy with 2D kinetic detection systems. FKM allows to examine photosynthesis of individual algal cells or on the cellular level of macroscopic structures. FKM is well suited to study heterogeneity in photosynthetic response among individual cells in algal cultures or in natural mixed populations. Special microscopic chambers allow to maintain live cells under optimal conditions for several hours or days and to follow evolution of photosynthesis in individual cells during the course of their growth cycle. Recent technical developments have allowed to determine FKM fluorescence kinetic with spectral resolution Detailed record
	Sborník pokroků nekonfokální a konfokální fluorescenční mikroskopie Pala, Jan Workshop in Ljubljana was focused on major aspects of contemporary widefield and confocal fluorescence microscopy. It covered topics from basic technical background of high-end optical microscopy to the most sophisticated applications. Detailed record
	Fluorescence methods for quantification of cyanobacteria Gregor, Jakub ; Maršálek, Blahoslav Fluorescence methods for quantification of cyanobacteria, their calibration, application and use. Detailed record
	Detection of cyanobacteria in raw water by in vivo fluorescence Gregor, Jakub ; Šípková, H. ; Maršálek, Blahoslav A method for fluorescence detection and quantification of cyanobacteria in raw water was implemented at the drinking water treatment plant. The procedure of implementation and calibration of the method is described. Detailed record

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