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Optical Measurement of Electromechanical Characteristics of Heart Cells
Čmiel, Vratislav ; Križanová,, Olga (referee) ; Babula, Petr (referee) ; Provazník, Ivo (advisor)
Dissertation is focused on the application of optical measurement methods using techniques of optical microscopy and fluorescence microscopy in measurements of electromechanical characteristics of isolated cardiac cells and clusters of differentiated cardiomyocytes. The first proposed method uses a practical combination of fluorescence microscopy equipped with fluorescent fast and high-resolution camera and atomic force microscopy for simultaneous measurement of calcium transients and contraction of cardiomyocyte clusters. The signals obtained undergoes filtration, processing and analysis. Result function parameters obtained by analyzing signals after application of caffeine are evaluated by comparison with functional parameters obtained during the control measurement. The second proposed method is applied to the cardiomyocyte clusters for the purpose of cardiomyocyte contraction signals measurement. The signals obtained by optical methods are analyzed and compared with the reference signal obtained using atomic force microscopy. Optical measurement method of cell contractins based on detection of cell ends using adjusting of microscopy images by re-sharpening and fluorescence method for cardiomyocyte contractions measurements were designed to increase realiability in simultaneous measurement of cell contractions simultaneously with calcium transients in isolated cardiomyocytes experiments.
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Právní a zdravotně sociální aspekty činnosti OSPOD jako ustanovených opatrovníků v zámu nezletilých dětí
BORSKÁ, Jana
The Czech Republic as a signatory of the Convention on the Rights of the Child has vested the practice of state administration in the field of care for minor children to municipalities with extended powers, where the protection of the rights and legitimate interests of minor children is safeguarded by state authorities of social and legal protection of children (ASLPC hereinafter). These authorities are incorporated to the extent laid down by the Act on Social and Legal Protection of Children (Act No 359/1999 Coll., as amended). The position and roles of ASLPC and their competence and jurisdiction are also regulated by the same act. The position of workers of these authorities and its legal embodiment is equally important. Regarding professionality, there are high demands on these workers. In the Czech Republic, significant changes in legislature have been made over the past three years. These amendments have led to the strengthening of the rights of minor children and to the establishing of new tools for their protection. In connection with the adoption of new substantive regulation of family law, which is now exhaustively dealt with by the Act No 89/2012 Coll., the Civil Code, new procedural rules have been established regarding protection of rights of minor children. Such procedural legislation includes (apart from the long-existent Act No. 99/1963, the Code of Civil Procedure) the Act No 292/2013 Coll., on Special Civil Proceedings. The decision-making regarding minor children has been vested mainly by the state into the competence of courts. The courts appoint the locally relevant ASLPC as a guardian who then represents the interests of minor children. The purpose, interwoven with and derived from the first one, was to explore the opinions of selected social workers of ASLPC and of district court judges regarding the defined problems occurring during the work of ASLPCs and courts while protecting the interests of minor children. These particularly include: the use of the tool of precautionary measure; problems of different territorial jurisdiction of courts and ASLPCs; processing of complaints regarding bias in various phases of the proceedings; professional training of workers of ASLPC It is evident from the proposals of both judges and ASLPC workers that it is necessary to unify territorial jurisdiction. The courts are suggesting the priority criterion of the address where the child mainly resides. ASLPC workers suggest the official permanent residence as the prime criterion. Bias causes problems in all phases of proceedings. It is obvious that courts take differing approaches towards the solution of this problem (some courts solve the problem of bias complaints filed by the parties while other courts do not) and for ASLPC workers it is difficult to assess how to act when such complaint is raised against them. The training of ASLPC workers - workers are obliged to educate themselves and their employer is obliged to finance such training. Not all ASLPCs are able to provide training in the required extent - due to financial reasons (the average costs of education of one employee pose from 9,169 to 13,400 CZK annually and they cover approximately 6 days of training). The heavy work-load caused by insufficient numbers of ASLPC employees prevents them from completing such compulsory training. As part of the examination of the "participation of a collision guardian in court proceedings" it has been found out that irregular participation of the worker in the proceedings (absence from participation in appeals proceedings); incomplete reports (directed to courts) from surveys in families. Based on comprehensive examination of the problems of territorial jurisdiction, it was recommended that requests are independently recorded and financial compensation is provided for ASLPCs who are requested to represent minors in front of courts, to draft proposals for precautionary measures.
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Comparison of optical methods for characterization of glass mosaic weathering
Kocour, Vladimír ; Petráňová, Veronika ; Valach, Jaroslav
The work presented in this paper is a part of research project supported by Ministry of Culture and devoted to conservation of precious mosaic of 'Last Judgement' on St. Vitus Cathedral in Prague. The aim of the project is to enhance external protective polymeric coating on glass tesserae of mosaic and also to develop optical method for assessment of coating’s surface conditions. The paper concentrates on comparison of various methods for surface evaluation assesses their advantages and disadvantages and also discusses their suitability for long term monitoring of coating state, namely reflectometry, scanning probe microscopy, nanoindentation, white-light interferometry, and scanning electron microscopy. It is found that white-light interferometry and reflectometry methods are the most suitable ones for monitoring purposes.
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Measuring phonological awareness – blend phoneme test in the beginning of schooling: an comparison of classical test theory and item response theory
Gorčíková, M. ; Šafr, Jiří
The main purpose of this paper is to show dis/advantages of two approaches classical test theory (CTT) and item response theory (IRT) to assess the level of diagnostic tools for evaluating phonemic awareness among first-grade students using longitudinal survey of children during the first grade of primary school (25 schools in Prague and Central Bohemia, 439 pupils). Phonemic awareness (FA) was measured by the task blend phonemes into syllables and words. The advantage of IRT compared to CTT is primarily a concept of independence of the test items. The probability of a response is dependent on the level of latent ability and characteristics of items. So if we know the characteristic of items parameter (difficulty) it could be estimated from the pupil´s responses the level of language skill for diagnostics. The results showed the need for blend phoneme test modifications (in particular for the end of the first grade) so as to adequately measure the high growth of FA ability levels during the first grade.
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The study of the influence of the single entry and pillar mining method in seam No. 40 in Jan-Karel protective shaft pillar, location CSA Mining Plant 1 on Jan and shaft No. 2 downcast shafts and on the CSA 3 upcast air shaft, surface and surface objects
Ptáček, Jiří ; Kajzar, Vlastimil ; Koníček, Petr ; Kukutsch, Radovan ; Waclawik, Petr ; Jiránková, E.
The presented method single entry room and pillar solves the possibility of applying single entry room and pillar mining method, where roadheaders are used, newly in two variants. According to the first of them will be entries and intra-entries inundated with a time lag with fly ash mixtures. The second variant will be excavated only basic entries with a width of 6.8 meters. They will not be flooded. The purpose of the study is also adding calculations of potential and the expected impact of single entry room and pillar method on the surface and surface objects and also the expected impact on the shafts in Jan-Karel protective shaft pillar.
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Determination of the company value
TALÍŘOVÁ, Klára
This diploma thesis was focused on determination of the company value in the chosen company. For this purpose was chosen two mostly used methods on the Czech market. First one was method of discounted cash flow and the second one was method of the economic added value. Main sources for all the calculation were annual reports and financial statements for the period of five last years from 2011 to 2015. These basis were used for developing a prediction for the following five year period till 2020.
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Cultural Centres
Adolfová, Barbora ; PROKOP, Petr (advisor) ; KAŠPAR, David (referee)
This thesis is concentrated on cultural centres built between years 1960 and 1990 in the former socialist Czechoslovakia. Six cultural centres have been chosen and analyzed to conclude the purposes they serve as well as the programs they offer. These chosen cultural centres are run as municipal organisations financed from the town budgets. The six towns where they are located are similar to each other in terms of their history, size and the time when their cultural centres were built. All of these centres share difficulties with the lack of cultural interest of local citizens, bad technical shape of the buildings in question and low budget.
The aim of this thesis is to compare the functioning of these six cultural centres and conclude whether being directly funded and influenced by the municipal councils has a positive impact and should serve as a model for cultural centres or should be replaced by a different one. It also focuses on cooperation between the management of cultural centres and municipal officials, cooperation with other local cultural institutions and cultural centres from different towns.
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Obesity in children of school age from the perspective of nurses working in GP
BURSÍKOVÁ, Drahoslava
Abstract Obesity in School Children from the Point of View of a Nurse Working in a GP's Surgery The thesis focuses on the problems of nursing care of children with overweight and obesity. This issue is very urgent nowadays, not only in the Czech Republic, but it is a global problem. Our country holds one of the leading positions in children obesity. Children suffering from overweight and obesity have not only health problems resulting from obesity complications, but also problems in the field of psyche and social relations, particularly among their peers. This is why the right insight into the issue and finding an acceptable solution for the future is so important. The theoretical part deals with the problems of children obesity, the specifics of nursing care of these children within a surgery of a general paediatrician. It also focuses on the needs of children with normal weight and on the needs of overweight children. Four aims were set within the research. The first aim was to map the situation in obesity among school and pre-school children in Benešov District. The second aim was to map services (possibilities of further assistance, treatment) that parents and practitioners may use in treatment of children with overweight and obesity in Benešov District. The third aim was to compare success rate of obesity treatment in children that have already undergone obesity treatment under specialist supervision and success rate of weight reduction in children trying themselves. The fourth aim was to map the problems of cooperation of a nurse with parents and school in treatment of a child with obesity. We used a qualitative research method in the research part of the thesis. The questioning method by means of semi structured interview was applied. Three research samples were prepared for the research purposes. The first research sample consisted of 10 nurses working in paediatrician practitioner surgeries in Benešov District. The second research sample consisted of 10 persons intentionally chosen from among parents of children with obesity that are trying to reduce the weight of their children themselves or under assistance of an obesity specialist. The children were of school and pre-school age. The third research sample consisted of 10 paediatrician practitioners practicing in Benešov District. Open-coding technique paper and pencil method was applied on interview processing. A document analysis was used for the mapping of the services in Benešov District and the whole Central Bohemia Region. Six research questions were set: 1. What are the possibilities of specialist help to obese children? 2. Are parents willing to help their obese children with overweight reduction? 3. Do parents of obese children also have problems with overweight? 4. What experience do nurses from paediatrician practitioner surgeries have with care about obese children? 5. What does cooperation between parents and a nurse from a paediatrician practitioner surgery looks like? 6. How does a nurse working in a paediatrician practitioner's surgery cooperates with the school? The thesis conclusion shows that the problem of children obesity is not just the matter of Benešov District, but it is a global problem, it is urgent and has an increasing trend.
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Social media influence
BAUEROVÁ, Milada
This diploma thesis deals with social media, specifically with what influence they have on an individual´s decision making while choosing a university. There are 87 universities in the Czech Republic, each of them offers unique study programmes and each one is specific, nevertheless, if they are divided according to subject-matter focus, for instance divided only into universities focused on the study of economy, it is difficult to differentiate which one will be the best choice for a possible student. For the purposes of this diploma thesis, University of South Bohemia has been chosen, and Czech University of Life Sciences Prague has been chosen for the purposes of comparison. The main goal may be specified as an analysis of social media influence during the choice of a university, and to design some useful recommendations based on the results. Other goals of this thesis are: - To find out how universities use social media, - To find out how students get involved in social media happenings. The results for this thesis will be obtained on the basis of a marketing research via a questionnaire survey among the students of the University of South Bohemia and of the Czech University of Life Sciences in Prague. Based on these results an analysis and a comparison will be carried out, finding out how strongly social media influences students when choosing a university, whether students themselves participate in social media happenings and what else may be improved by the universities.
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The prevention of pathological occurence of burnout syndrome (an example of employer)
BLAHOVÁ, Nela
This bachelor thesis deals with the issue of burnout syndrome which is currently a very discuss frequent problem. The rate of working stress will be investigated in selected groups of staff in company COOP družstvo HB. The purpose of my thesis is to compare which group of staff is threatened by burnout syndrome the most. The work is focused on the economical aspects which are reflected particularly in human capital. There will be applied the quantitative research, concretely questionaire research. Based on the results of questionaire research will be applied analytical method of the job evaluation, which is one of the main issues of human resources management. This application will be also recommendation for prevention for the company.
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