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Expression of selected proteins in sperm in men with normal and pathological spermiograms using monoclonal antibodies
Pěknicová, Jana ; Čapková, Jana ; Dorosh, Andriy ; Margaryan, Hasmik ; Kubátová, Alena ; Děd, Lukáš
Recent studies show that infertility in human populations it affects an estimated 15% of couples of reproductive age. Male infertility is the primary cause for 60% of these cases. For these reasons, we analyzed the acrosomal and sperm surface proteins in men with normal and pathological spermiograms. We found that intra-acrosome proteins: TERA (Transitional endoplasmic reticulum ATPase), GAPDHS (Sperm Glyceraldehyde-3-phosphate dehydrogenase), and PRKAR2A (C-AMP-dependent protein kinase II, PRKAR2A), which can be identified using our monoclonal antibodies, are different express in healthy men and men with astenozoospermia (with reduced sperm motility), and with a significantly reduced expression in the astenozoospermia. These proteins are involved in energy metabolism and apoptosis of the cells, and some of them in the sperm-egg interaction; therefore, they have an important role in reproduction. On the other hand, there were no statistically significant differences in the expression of surface proteins (Appolipoprotein J (Clusterin) and Semenogelin). Our findings show that astenozoospermia as a complex disorder of the semen is often combined with other pathological conditions that are not diagnosed by the semen analysis. Therefore monoclonal antibodies are so suitable instrument for the detection of proteins associated with the pathology of the sperm in the semen with low sperm motility. In general, monoclonal antibodies against the sperm proteins are an appropriate tool to detect sperm quality in reproductive medicine.
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The role of a nurse in a prevention and a treatment of intestinal parasites of children
JANDOVÁ, Anna
The best-known intestinal parasites are Pinworms, Roundworms, Tapeworms and the disease called Toxocarosis. The oftenest parasite is, according to sources, the Pinworm. Intestinal parasites affect mostly young children of pre-school age, sometimes even older. The first goal of the thesis was to investigate parents' awareness of the prevention of parasitic diseases in children. There is a hypothesis set to this goal: Parents of children who suffered from parasitic disease are more informed than parents of children who have not had this disease. The second goal was to map the specifics of nursing care for children with parasitic diseases at GPCHA practice. A research question was chosen for this aim: What are the specifics of nursing care at GPCHA in parasitic diseases? In methodology, the empirical part was chosen and it was a qualitative - quantitative research survey. For the quantitative part, the interrogation method and technique of non-standardized questionnaire was used. The research sample of quantitative survey consisted of 223 respondents, it means parents whose child is aged from 0 to 6 years. The questionnaires were distributed on social networking and others in a nursery school in Týn nad Vltavou. At the very beginning, respondents were acquainted with the topic of the thesis. The results of a quantitative survey were processed with the help of a data matrix and were further processed within twenty-arranged bar charts. To verify the hypothesis, we used the Chi- Quadrat test, which in this case is not satisfactory, therefore, there had to be chosen to merge the responses. An interrogation method, technique-depth interview was used in the qualitative part. The research group consisted of five nurses; three nurses working at GPCHA in Týn nad Vltavou and two working at GPCHA in České Budějovice. While processing the interviews, there open coding was used and an analysis of interviews was conducted by using the method of Pencil and Paper Interview. The results of this bachelor thesis will be published in the journal Paediatrics practice.
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Differentiation of offers and methods of working physiotherapy of persons with a mental or combined handicap in a practice of social service providers and another subjects.
POŠMUROVÁ, Nikola
In the Bachelor's thesis I have dealt with differentiation of tenders and rehabilitation methods. Specifically, individuals with mental or multiple disabilities and for providers of social services and other bodies. In the theoretical part I focused on defining the basic terms relating to that issue. In the introductory chapter I dealt with the definition of the concept of mental disability, causes of its creation and definition of degrees of mental retardation. The next chapter is devoted to the combined disability, specifically the yoke of the definition, causes of and classification. In the third chapter is devoted to the importance of the work, the status of the work in a person's life and the status of work for persons with disabilities, including individuals with mental or multiple disabilities in Bachelor thesis I. In the next chapter, I deal with comprehensive rehabilitation system, specifically the remedies rehabilitation, educational resources, social resources, rehabilitation, rehabilitation and special attention devoted work resources, rehabilitation, which will directly affect the Bachelor thesis. Furthermore, I determine a target group the work of rehabilitation and the preconditions for its implementation. In other chapters is devoted to the specification of tools and resources work rehabilitation, employment policy tools and I define jobs that are offered to persons with intellectual disabilities. In the practical part of the thesis, first determine the main aim and target work. The main goal was to determine what are the menus and methods of rehabilitation for people with mental or multiple disabilities with providers of social services. As the target I had set to determine what are the possibilities of transmission from individual to social services on the open employment market. Specifically, I've focused on the resources provided by the rehabilitation of daily, weekly care centers or day service center, in addition to the socio-therapeutic workshops, in a sheltered employment, and employment on the open employment market. To meet the objectives I used a qualitative approach, specifically the semi-structured interview with six providers of social services of the South Bohemian region The analysis of the information obtained, I discovered that if the device is operated daily, weekly social welfare institution or Centre, day services, the services provided are identical, not different from each other. In the case of socio-therapeutic workshops is the diversity of the wider, each device provides a variety of services, different options, some devices have socio-therapeutic workshops directly on the device, others have socio-therapeutic workshops and externally, outside of your device. Protected jobs are also in the majority of providers of social services offered both within and outside the device, always the odd jobs, such as in the kitchen, cleaning, in teahouses and the like. It always depends on the type and degree of disability, on the basis of a levy for the client to the appropriate activities. Location of the client on the open employment market is not very common in most devices, some devices that offer clients or do not provide. Partial objective was to detect transmission, I found that the only limiting factor is the client's disability that limits its options and device options, of course, that the services it offers and delivers. The aim of all devices is to achieve the greatest possible degree of autonomy of the client and allow it to advance from care centers or day service center in the socio-therapeutic workshops, found a protected job, and a job on the open employment market.
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Role of the nurse in screening for depression in the elderly.
VRZALOVÁ, Monika
The diploma thesis deals with problems of depression in older people. Mainly the work is focused on identifying and analyzing the role of nurses in screening for depression in older people in primary care, acute care, long-term care and home care. This thesis was focused on theoretical direction and was used the method of design and demonstration. In this thesis was set one main goals with five research questions. The main goal was to identify and analyze the role of nurses in screening for depression in the elderly. RQ 1: What is the role of the nurse in screening for depression in the elderly? RQ 2: What is the role of the nurse in the primary care in screening for depression in the elderly? RQ 3: What is the role of the nurse in screening for depression in hospitalized patients in acute care? RQ 4: What is the role of the nurse in screening for depression in seniors in long-term and home care? RQ 5: What rating scales and methods are used in screening for depression in the elderly? The thesis introduce the concept of depression. The following are specified the causes of and the important factors that affect depression in the elderly. It also deals the differences in the clinical symptomatology of depression in old age. It explains possibilities and various barriers in the diagnosis of depression. Another chapter introduces complete geriatric examination, diagnostic classification systems, possible screening methods and scales for detection of depression in the elderly population. It also deals methods of pharmacological and non-pharmacological treatment and its possible complications associated with older age. By reason of increased suicide rate caused by depressive disorder the issue of suicidal behavior in the elderly is introduced. The next chapter deals with the nursing process, which is used by nurses in practice. It consists of the evaluation of the patient's health condition, making nursing diagnosis, creating nursing plan and subsequent implementation and evaluation. The nursing process is also needy for providing quality care. The nursing process in the stage of nursing diagnosis, introduces possible nursing diagnosis for a patient suffering from depression, which are based on the latest classification. Finally is described the role of nurses in screening for depression in the elderly in different health facilities and their contribution to the timely evaluation of depression in the elderly. This chapter introduces the role of nurses, nursing screening and collaboration with a physician. The role of nurses in screening for depression in different medical facilities is based on the first phase of the nursing process of assessment. On the basis of objective and subjective information, the nurse will assess the overall health and mental condition of the patient. Primarily, it was investigated what is the role of the nurse in screening for depression. On the basis of content analysis and synthesis it was necessary to used and processed domestic and foreign literature. A number of relevant sources are the results of various studies and Meta-analyzes, mostly from abroad, but also from the Czech Republic. The thesis can serve as a basis for nurses. The result of this thesis is to create e-learning material available for students in the Faculty of Health and Social Sciences of South Bohemia in Ceske Budejovice in the tutorial called Moodle.
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The need for security and safety in connection with a planned surgery with the patient's perspective and nurses
BOHDALOVÁ, Jaroslava
A planned operation performance always affects patient´s needs for assurance and safety. It is important to provide necessary information connected with the planned operation performance itself so that the patient can feel assure and safe. The key role for the patient and her/his needs for assurance and safety is in general nurse´s hands because she is the closest person to the patient and can help to inspire her/his needs for assurance and safety with konfidence best through the aid of her knowledge and skills that she should have as well as through the aid of her attitudes. The target of the thesis was to find out how a planned operation performance affects patient´s needs for assurance and safety and how a general nurse inspires patient´s needs for assurance and safety connected with a planned operation performance with confidence. The following research questions were stated: How does a planned operation performance affect patient´s needs for assurance and safety? How does a general nurse affect patient´s needs for assurance and safety connected with a planned operation performance to inspire her/him with confidence? What is insufficient in filling patient´s needs for assurance and safety before a planned operation performance? How can a general nurse help a patient control her/his fear and state of anxiety related to a planned operation performance? The method qualitative research with the use of depth interview was used to figure out the investigation. The respondents were willing to record the interview which were verbally rewritten and then subjected to the contents analysis by the method paper and pencil. From the research it is clear that a planned operation performance affects patient´s needs for assurance and safety in a negative way disregarding to her/his previous experience. Patient´s worries and negative feelings can be eliminated by a general nurse who treats her/him as equal, taking up an individual approach, informing her/him about all performances and proceedings, having a calming and welcoming way of communication, using empathy and listening as well as trying to meet patient´s wishes according to her possibilities. The research reveals the advantage of patients´ readiness for planned operation performances. Patients can learn it in the framework of educational proceeding in the out-patients´ department at the same time when they learn that they are going to be operated. The research also shows that general nurses do not have enough knowledge in the sphere of therapeutical communication. The results indicate that it would be suitable to start educating patients as soon as it is known how their disease will be solved and treated in order to reduce the negative impact of surgical treatment to patients´ needs for assurance and safety. General nurses should complete their knowledge and skills to be able to help patients to cope with their difficult life-situation. On the basis of the research investigation and after the consultation with the senior nursing officer, the training course will be organized. The topic of the training course will concentrate on communication with patients before planned operation performances with a view to therapeutical communications and therapeutical interviews. Other discovered facts, for example, the lack of information provided to patients and possibilities regarding better patients´ readiness for planned operation performances will surely be solved by the hospital management.
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Stress situations and nursing personnel
MAREŠOVÁ, Lucie
This thesis deals with the stressful situations in the profession of the nursing staff. The occupation of the nurse belongs to the most demanding ones. In this occupation, the nurse is permanently affected by many stressors of different types. She faces death, the suffering, dying and incurably ill people very often. She is there in times of pain, agony and suffering. Personal encounters with these difficult situations require highly professional approach but most of all, they present immense work stress for the nurse. The theory section describes mainly the stressful situations that are the most common and most difficult in the work of the nursing staff. The next part of the theory section describes possible consequences of the stressful situations and methods of managing them including the support by the management. This thesis has two objectives. The first objective was to map the manners used by the nursing personnel to cope with the stressful situations brought by their profession. The second objective was to find out the difference in the coping with the stressful situations between the hospital staff and hospice staff. The research section of this thesis contains the quantitative research done using the technique of an anonymous questionnaire with 20 question prepared by ourselves. The questionnaire was distributed to the nursing staff of all categories in the hospital and hospice care. It was completed by 269 respondents in total. The data was statistically processed using systems Microsoft Excel 2010 and Software R, version 3.0.2 (Chi-squared test, Fisher´s exact test, and Wilcoxon test). We determined four hypotheses. H1: Manners of coping with the stressful situations depend on the qualification of the nursing staff reached; not confirmed. H2: Manners of coping with the stressful situations are influenced by the length of practice of the staff; not confirmed. H3: Manners of coping with the stressful situations differ between the hospital staff and hospice staff; confirmed. H4: The nursing staff views their own mental hygiene as more efficient than the support from the management concerning the coping with the stressful situations; confirmed. The research results show that the nursing staff faces the stressful situations very often. They see as the heaviest stress the care of the suffering and aggressive patient, conflicts with the patient´s family, conflicts at work and workload. The manners of coping with the work burden which proved as the best for them are relax, rest and sleep. There were no differences proved in the manners of coping with the stress among respective members of the nursing team. Nevertheless, the coping with the work burden of the nursing staff in the hospital and hospice care is different. A minimum of the respondents notices any support during the stressful situations from the employer. On the other hand, certain support by the management could be viewed in the form of trainings, supervision or contribution to a "recovery" which are granted to the respondents from the employer according to their answers. However, the respondents would prefer, as the support by the management, the increase in pay or more days off. The respondents view as insufficient the preparation of the graduates for the future stressful situations in their profession, as well. They would recommend mainly various trainings dealing with the stress and its management. A good solution to alleviate the work burden of the nursing staff and quality enhancement of the services provided could be investments into better work conditions, both in terms of sufficient human and material resources and the very organization of the work processes.
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Narcolepsy and other seizure disorders
TONDLOVÁ, Andrea
The thesis deals with the issue of seizure disorders from the perspective of special neurology. In terms of content, it is focuses on two major areas. The first part is theoretical in nature and has descriptions of the individual disorders, their clinical manifestations, diagnoses and current treatments. It is focused on the following seizure disorder group with regard to one of the objectives of the work: epilepsy, tetany, Meniere's syndrome, migraine and more specifically narcolepsy. These findings are supplemented with information on current knowledge regarding the treatment and diagnoses of these diseases, which make the lives of thus disables people very difficult and prevent them from leading their lives the way they could if they were in full health. The aim of the work is not just a theoretical description of the currently available knowledge of these diseases from literature, but also a practical confrontation thereof from experiences, which in this case result from the true stories of people suffering from seizure disorders. A specific seizure disorder has been selected for this second part of my thesis, which needs to be talked about more in order to become better known. It is a disorder that is interchangeable with regard to its symptoms, which are similar to other disorders, and it is difficult to diagnose or is only diagnosed after an inconveniently long time - i.e. Narcolepsy . The thesis therefore deals with the issue of narcolepsy in most detail, with regard to the practical focus in the next section, where in addition to aforementioned basic knowledge, the author describes the activities and the importance of sleep laboratories and centres as institutions involved in treatment. In order to penetrate into the issue of this disorder even more deeply, she adds the aforementioned anonymous stories of people affected in this way. This part is also complemented with illustrative images of the sleep laboratory and the stories of these people as a better visual complement of the thesis for the reader. A descriptive analysis (comparison) of each symptom was chosen as a comparative method of the aforementioned thesis objective (to compare the theoretically given symptoms of narcolepsy with the symptoms of individuals suffering from this disease), in which the goal was to choose the common symptoms these people which make their lives difficult and compare these data with information (symptoms of narcolepsy) found in contemporary literature. In conclusion, we visit the most important information from scientific literature, the views of the professional community on the current research and studies on narcolepsy, i.e. the specifically examined causal links of applying the Pandemrix vaccine (against swine flu) and narcolepsy and a summary of the results of other studies investigating the etiology of this disease. The aim of this section is to present the reader with, and identify, the current developments in narcolepsy research. An evaluation of the historical development of interest in sleep disorders and an evaluation of the conclusions drawn by comparing symptoms specified in professional literature on narcolepsy with actual symptoms of people suffering from this disease are also included. The aim is to provide the reader with a bio-psycho-social view of a person with this disease, which is not always emphatically discussed in every piece of professional literature. The result of the entire thesis is a summary of the current state of knowledge of these diseases and a reflection on the research of the current possible causes of narcolepsy. It continues to compare the theoretical background of the narcolepsy disorder with the experience of living with this disease, including stories of selectively chosen individuals suffering from narcolepsy.
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Pick´s disease and the specifics of the nursing care of a patient with this disease
KOPKAŠOVÁ, Lenka
Pick's disease is a degenerative-atrophic process that primarily affects the frontal and temporal lobes of the brain. Besides Alzheimer's disease, it is a lesser known form of dementia which affects people of younger age. Although the exact range of Pick's disease occurrence varies in the literature, the average reported age is between 50-60 years. The disease can change eating habits, cause emotional swings, inappropriate behaviour and sloppy appearance of the patients. The behaviour to others is often selfish, the patients are unable to listen to others and ignore their surroundings. Pick's disease is also characterized by inappropriate sexual behaviour. Globally, the number of dementia patients is still growing and according to expert guesses the increase will continue. Nursing care for patients with dementia is therefore becoming a very actual topic as dementia is called a quiet epidemic of our century. Nurses in their departments quite frequently encounter patients suffering from dementia of various origins at different stages of disability and the staff at healthcare facilities often regard these people as a burden. The aim of this thesis, which is called Pick's Disease and Specifics of Nursing Care for Patients with this Disease, is, based on the theoretical knowledge, to map definitions and diagnosis of Pick's disease, to define the needs of such a patient, and to list the most common problems in the nursing care. It focuses on people with dementia within medical facilities. For proper understanding of the problem, the thesis starts with the anatomy and physiology of the brain and the description of cognitive functions which are affected by the dementia syndrome and the definition of dementia. Based on the studied literature, dementia is divided according to various criteria. It has been found out that dividing dementia is not entirely uniform, but the essential facts and overall opinions of the authors do not diverge. Pick's disease belongs to a group of frontotemporal dementia and its position in this group has changed over time, as it is recorded in the literature. The thesis includes recommendations resulting from the studied literature in the approach to patients with dementia and emphasizes personal communication with the patients and empathetic and humane approach. Although medicine cannot prevent dementia, an early diagnosis of a specific type of dementia and correctly targeted treatment can increase the quality of life not only of the patients but also of their families. I have chosen this topic because I find it very current and interesting. During my nursing practice at the Surgery Department and Aftercare Department, I have met patients suffering from dementia and I did not know how to properly and efficiently communicate with them. For the theoretical part of my work, the methods of explanation, synthesis and induction on the basis of Czech and foreign information sources have been used. The most frequently quoted Czech authors in my thesis are doc. MD. Roman Jirák, PhD., the head of the center for diagnosis and therapy of Alzheimer's disease at the psychiatric clinic of the General University Hospital in Prague, the 1st Medical Faculty of Charles University. This thesis can serve as an informational and educational source both for nurses and the general public who may experience the disease within their families. It would be considered a great success if it helped nurses or family members to diagnose Pick's disease or other dementia at an early stage.
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Ability of dogs to discriminate human odor exposed to chemical detergent
Čajágiová, Martina ; Vyplelová, Petra (advisor) ; Jaroslav, Jaroslav (referee)
In the recherche part of our thesis we familiarize ourselves with the human odor, theories of its origin, definition, anatomy of human skin and odors, composition of individual human odors and with odor secretion. We also look closer on the topics of odor absorbent and its use in the world, the transmission of odors to odor sensors and securing of scents. This section of our thesis discusses the resistance and survival abilities of odor, scent identification method and its history, organic acids, and defines the application of laundry detergents and ultrasonic washers. Aim of our thesis was to verify the relevance of the use of chemical detergents in the purification process when working with scents. We tried to verify the ability of detergents to degrade the human scent on odor absorbents to such an extent, that specially trained dogs would not be able to identify it. Our experiment was following a precise determination methodology. In the first phase samples were collected from the hands of targeted persons to a scent carrier - a metal cylinder (extirpate odour), by one researcher. In the second phase samples were collected from the body to a textile carrier, by another researcher. The metal scent carriers were processed. Some of them were left as they were (control sample) and some were exposed to chemical detergents with and without usage of an ultrasonic washer.
In the experiment where we tried to identify the odour samples, six bitches of German shepherd were used. They were specially trained for odour identification. Each dog was let three times to identify the target scent exposed to a detergent and three times to identify the target scent not exposed to a detergent. Target smell was randomly deposited between other samples and its position was changed, so that the handler did not know its position and thus was unable to affect the work of his dog. The indicator of positive identification was a sign the dogs were taught - to sit or lay in front of a sample.
Any dog was unable to identify the scent which was exposed to chemical detergent and all dogs identify the scent unexposed to a chemical detergent. Our experiment has shown that the use of chemical detergents in the purification process when working with scents is relevant to the degradation of individual human scent.
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Analysis of beer consumption preferences
Fontánová, Klára ; Hlavsa, Tomáš (advisor) ; Jindrová, Andrea (referee)
The main subject of this thesis is analysis of preferences in beer consumption. The main goal of the thesis is to discover and evaluate factor which could affect consumer's behaviour. Therefore, t2he basis for this thesis is a questionnaire which was prepared according to marketing research principles.
The first part is about beer and marketing research. The part about beer contains main historical data, a brief brewing process description, including main ingredients and beer types. Next, there are mentioned main Czech breweries. Inconsiderable part are also factors which affect beer consumption. In the part dealing with the marketing research are described the steps, methods of data collecting ad data resources.
The second part is about the questionnaire itself and its evaluation. The questionnaire had 322 respondents.
Firstly, the results are summarized and then are tested based on null hypothesis.
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