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Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head
Šebková, Nataša ; Frolíková, Michaela ; Děd, Lukáš ; Dvořáková-Hortová, Kateřina
CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction (AR). It also associates through membrane integrins with specific MAP kinases involved in the AR. Our aim was to monitor the dynamics of relocation of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. The dependence of this localization changes on the dynamic of actin cytoskeleton was studied. Our results show the changes in the localization of these proteins associated with the AR and their co-localization was observed using proximity ligation assay. After the AR CD46 and β1 integrin spreads across the sperm head, entering the post-acrosomal compartment, and permeates the borders of different domains. It was shown previously that actin dynamics is necessary for acrosome reaction-associated translocation of Izumo1 protein that is required for sperm-egg fusion. Therefore Latrunculin A was used during sperm incubation. The co-incubation of capacitated sperm with Latrunculin A leads to a decrease of the percentages of sperm, which express relocation pattern after induced AR. 3D models and visualizations of potential membrane processes responsible for the relocation of proteins from the acrosomal area to the other compartments of the sperm head were prepared. Our results deliver new information that proteins CD46 and β1 integrin undergo dynamic relocation towards the sites of sperm-egg fusion during the AR in vitro. The inhibitor of actin dynamics abrogates significantly the AR-associated changes in proteins localization. We speculate that this relocation is of importance for the successful sperm-egg interaction, adhesion and subsequent gamete fusion.
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Assessment of compliance with quality management system requirements and benefits of its implementation in a specific organization
Nálevková, Petra ; Hykš, Ondřej (advisor) ; Reuterová, Hana (referee)
The aim of this thesis is to evaluate on the basis of information provided by IDC - FOOD, Ltd. the fulfilment of requirements for ISO 9001 quality management system and identify possible areas for improvement. Furthermore, the aim is to assess the impact the introduction of the quality management system on the functioning of society. The first part of the thesis deals with the theoretical definition of quality and development of care of quality. It also describes approaches to quality management and their development. In the next section IDC - FOOD, Ltd. is introduced, and further it is discussed how the organization implemented and fulfils the requirements of ISO 9001, also possible improvements are identified. At the end of the practical section the impacts of implementing quality management system are then summarized.
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Assistance as compensatory and supporting means of the special pedagogy
PÝCHOVÁ, Lucie
My Bachelor thesis is focused on the topic of "Assistance as a compensation and support to special pedagogy". The aim of my bachelor thesis is to complete theoretical knowledge related to the assistance phenomenon and to analyse the occupation of personal assistant. The aim of practical part of my bachelor thesis is to implement the comparison of legislative determination and provable working activities carried out by pedagogical and personal assistant. In accordance with the aimes specified above has been established two partial aims. 1st aim: to examine what the assistants´ work motivation is. 2nd aim: to examine the level of assistants´ self contentment.Thesis is devided into two parts theoretical and practical one.Theoretical part is devided into three units. First unit defines the key words: assistance, compensation and support, special pedagogy, social integration and inclusion. Second unit is focused on personal assistance itself. Related key words are defined as well. In this unit I also describe a historical development of the personal assistance process, working demands placed on potential assistant, possible complications related to mentioned occupation and of course everyday work routine. Third unit is focused on the personality of assistant and assistants themselves. I describe the historici development of the assistants´ personality, work requirements, work duty and cooperation with parents and educational institutions. Practical part contains the specification of work aims, methodology of investigation, related data gathering process and of course the data analysis. I interviewed pedagogical assistants just as the personal ones. All the data and results contained in my bachelor thesis can be used for needs of any related organisation recruiting new assistants, because it contains informations which could be useful when hiring people. My thesis can be also used as a background material for any further students.
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Nursing procedures in taking blood pressure
SEDLÁKOVÁ, Tereza
By blood pressure is meant lateral pressure or the side pressure of a blood column on the side of a vessel. The pressure is created thanks to the action of heart as a blood pump. This pressure is not the same in different parts of the bloodstream. By the blood pressure is meant the arterial pressure or pressure in the artery, so the pressure in big blood vessels. The highest values are reached in the expulsion phase of the heart action, which is called systole. The lowest values are reached in the phase of filling heart chambers, which is called diastole. The sole nursing procedures required in the process of taking a blood pressure reading are many and all must be followed. This thesis is composed of a theoretical part and practical part. Introduction to the theoretical part is composed of the main terminology from the physiology of blood pressure. In the theoretical part we follow with division on hypotension and hypertension, which are the most common diseases related to blood pressure, with diagnosis and recommendation for treatment. This thesis also offers information about measuring blood pressure by the invasive and the non-invasive method. The theoretical part is focused on the importance of the nurse in the process of measuring blood pressure with emphasis on her knowledge and practical skills.
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Palliative care is provided by patient's family at home
KUČEROVÁ, Tereza
The thesis, titled Palliative Care Provided by Patient's Family in Home Environment, discusses the specific care needs of a terminally ill patient in his/her own home. Caring for an ill person in a home environment is beginning to be a growing trend in home care, but also it is an increasingly common wish of the patients themselves. The family is therefore required to have enough theory and practical knowledge in order to deliver a highly demanding personal care for their close family members. The aim of the thesis is to summarize the current situation of delivering palliative care in home environment in Czech Republic whilst concentrating on the specific needs of the patient and the needs of their family. The objectives of the research were to determine whether family members were introduces to the specifics of palliative care of terminally ill patients; to map their skills in delivering palliative care in home environment and to reveal the most common issues that family members come across in that home environment. The final objective was to map out services in regions of Central Bohemia and Liberec that are accessible to family members whilst caring for a terminally ill patient. The research part of the thesis was completed employing a method of qualitative investigation using semi-structured interviews with patient's family members and nurses providing hospice care in home environment. It emerged from the analytical interviews that the family's knowledge level regarding support for palliative care in home environment is very limited and in hospitals families are only given minimal or no support or information. As a final document for the thesis an informative educational brochure was created based on the results of aforementioned research, primarily targeting patient's family; its core objective is to improve information and collate the specifics of care that would allow the patient a dignified end to their life.
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Nursing care management of patients after intrapleural chemical pleurodesis
KIESEWETTEROVÁ, Renáta
The pleurodesis is a treatment method for patients suffered from the symptomatic malignant pleural effusion. The goal of the pleurodesis is to close a pleural area by connection of visceral and parietal pleura in order to reduce or terminate production of pleural effusion. The sterile talc powder is the world's most used agens within a practice. After a parental analgesic is the powder (as a sllury) either applicated into a pleural cavity through the chest drainage or surgically dispersed over the treated (via abrasion, decortication) pleural space during the thoracoscopy/thoracotomy. This diploma thesis focuses on the treatment technique of bedside talc-pleurodesis through the chest drainage. The nurse has an irreplaceable role in the whole process from a preparation of patient to assistance by intrapreural application, to care of patient after the operation or serves as a physical support during potential complications. The aim of the presented work is to detect priorities in nursing care and find out whether patients need increased care after intrapleural therapy. The qualitative research method was chosen for the analysis where semi-structural interview with open questions were used. We created case reports analysed with the adaptive model of Callista Roy as well. The research sample contained patients chosen with respect to specialization of researched problem. We set two basic focal stimuli according to the analysis of respondents´ reactions. These stimuli serve as a basis for determination of priority parts of the nursing care by patients after bedside pleurodesis. We defined the need to be without pain and the need to ensure safety and certainty as a central focus of the nursing care. Among secondary fields of nursing care were identified sleep disturbances and limited mobility, which leads to reduction of self-care in getting dress, hygiene as well as in defection and miction. We found out within the second goal identical intensity of the nursing care before and after bedside talc-pleurodesis. This statement is based on comparison of interventions before and after aplication analysed via adaptive model by Roy. Nevertheless, causes of interventions were modified to a certain extent. The main goal of diploma thesis was to map requirements for high-quality nursing care related to bedside talc-pleurodesis via chest drainage. The work should extend theoretical basis not only for nurses in clinical practice.
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Iniciace pohybu byčíku, signalizace a regulace pohyblivosti spermií ryb: fyzikální a biochemické řízení
PROKOPCHUK, Galina
The current study attempted to shed light on the regulatory processes and response arrangements of fish spermatozoa during the course of maturation and motility initiation. The first intent of this study was to improve the understanding of the mechanism underlying the acquisition of potential for sperm motility in sturgeon. Up to present work, the physiological process underlying sperm maturation in this species has not been described at all. Our results showed that sperm maturation in sturgeon occurs outside the testes because of dilution of sperm by urine and involves the participation of high molecular weight substances as well as calcium ions present in seminal fluid and/or urine. The second aim of the present study was to investigate the coping mechanisms in fish spermatozoa with osmotic and ionic activating mode, as well as in spermatozoa of euryhaline fishes, to various environmental conditions. We showed that alteration of environmental osmolality might affect the fish sperm in different ways, depending on fish species and modes of spermatozoa motility activation either osmotic or ionic mode. In response to osmotic stress caused by hypotonicity, carp spermatozoa regulated the flow of water across their cell membrane and increased their cytoplasmic volume during their short motility period. In contrast, no indications of sperm volume changes were observed neither in sterlet nor in brook trout spermatozoa, both of which having an ionic mode of motility activation. We also examined the mechanism by which sperm motility triggering in euryhaline fishes can adapt to a broad range of environmental salinity. Our results demonstrated that spermatozoa of euryhaline tilapia, Sarotherodon melanotheron heudelotii, reared in fresh-, sea- or hypersaline water can be activated in hypotonic, isotonic or hypertonic conditions of swimming milieu, provided Ca2+ ions are present at various levels. It was established that the higher the fish rearing salinity or the more hypertonic ambient media at spermatozoa activation, the higher extracellular concentration of Ca2+ ions is required. The results obtained in the present study allow suggesting that osmolality is not the main factor inhibiting sperm motility inside the testis in the S. melanotheron heudelotii. A third aim of this study was investigation of the regulation of motility initiation process and description of flagellar beating initiation in chondrostean spermatozoa. We detected that K+ inhibition of sperm motility in sturgeon can be by-passed due to the pre-exposure of sperm cells to a high osmolality shock prior to its transfer to K+-rich swimming media. Thus, we hypothesized that sturgeon spermatozoa may be activated by use of an unexpected signaling pathway, independent from regular ionic stimulation. The successive activation steps in sturgeon spermatozoa were investigated by high-speed video microscopy, using specific experimental situation, where sperm motility initiation was delayed in time up to several seconds. At motility initiation, the first couple of bends formed at the basal region begins to propagate towards the flagellar tip, but gradually fades when reaching the mid-flagellum. This behavior repeats several times until a stage where the amplitudes of bends gradually reach similar value, what eventually leads to sperm progressive displacement. The total period needed for the flagellum to switch from immobility with rigid shape to full activity with regular propagating bends ranges from 0.4 to 1.2 seconds. In conclusion, the results of the current study bring valuable pieces of information into the general understanding of the processes of maturation of fish spermatozoa, their adaptability to different physical and biochemical circumstances, the extra- and intra-cellular signaling as well as the regulatory mechanisms of motility activation in fish spermatozoa.
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The introduction of quality management system according to ISO 9001
Kunzová, Kateřina ; Štůsek, Jaromír (advisor)
The thesis describes the design process and the documents required to meet the standards associated with the effective implementation of a quality management system according to DIN EN ISO 9001 in organization Zinková slévárna - Ing. Karel Dítě.
In the theoretical part of the work I explain the basic concepts and issues relating to the use of literature associated with the ISO 9001 Quality Management System. This section also describes the general process for implementing the standards within an organization. Following on from this, I have used the knowledge gained from the theoretical section within the practical part of the thesis.
The practical part of my work includes a description of the selected organization from several perspectives - it is a focus, organizational structure, customer and supplier portfolio. This section also analyses the current state of the organization and includes a proposal to meet the ISO standards. The final section contains the documents necessary for the implementation of ISO 9001 within the organization, so that the quality management system corresponds to the prescribed standards.
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Innovation and Business Project
Teska, Jaroslav ; Moulis, Pavel (advisor) ; Michal, Michal (referee)
The aim of this diploma work is the creation of specific business project dealing with establishment of mensurational office. The literary research contains the definition of running a business and lying the foundation of own business project preparation. This basis contains recommended methods, requirements of business project and the structure.
The core of the diploma work deals with creation of specific business project on the basis of literary research knowledge. The first is the description on mensurational office following the analysis of surroundings on the basis of makro-surroundings and micro-surroundings analysis of the specific mensurational office. The next step is the description of business opportunities, human resources and marketing strategy. The conclusion of diploma work is dedicated to economic evaluation and reality of business project.
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Evaluation of genotypic response of winter wheat production interventions
Bláha, Tomáš ; Faměra, Oldřich (advisor) ; Pavel, Pavel (referee)
Sources used in this thesis concerns the cultivation, the development and the growth of wheat and its recommendation for agro-ecological conditions such as requirements for the crop, influence of the agricultural technologies to underground parts, fertilization of wheat, the optimum sowing date and sowing depth. In the next part of literature search there is an information about response of winter wheat for chemical protection including treatment morforegulator of growth. The end of literature search deals with the composition of wheat grain and its potential influence to quality.
The methodological part is devoted to independent description of characteristics of the varieties which are included in experiment. Data for evaluation are based on literature published by ÚKZÚZ. This part summarizes the equipment used for conducting an experiment. Equipment consists machinery and laboratory equipment.
The experiment was located at two places - in Úhřetice near to Chrudim and in Krukanice near to Plzeň. Both places are valuated in the essay - description of altitude, soil type etc.
Evaluated results of experiments are at the end. The conclusion of my thesis is that more comfortable climate for growing wheat is in Úhřetice - the average yield is for almost 20 % higher than in Krukanice. Other conclusion is about late sowing date which reduces yield and about increasing number of antifungal treatment increases the yield. Each of the varieties are valuated in the next part and the best treatment options for them are recommended. One fungicide treatment is proposed resistance to disease (Bohemia, Seladon), dual fungicide treatment is recommended for the rest of varieties. Field evaluation of disease shows that intensity of the treatment must respond to the current situation (according to the year).
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