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Whey utilization with thermophilic microorganisms
Rychová, Alexandra ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This diploma thesis studies the utilization of whey using thermophilic bacteria of the genus Thermus and Geobacillus. The whey stripped off proteins was used as a cultivation medium during experiments. The cultivation took place in the Erlenmeyer flasks, to assess the optimal conditions for microrganism’s growth. During the cultivation in the bioreactor, growth curves were established. The amount of whey utilization was assessed by analytical methods that determine the concentration of reducing saccharides (lactose) and chemical oxygen demand (COD) while studying the optimal conditions and a method determining the concentration of reducing saccharides during growth curves analysis.
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Preparation of selected microbial metabolites from waste materials
Jechová, Iva ; Vítová, Eva (referee) ; Babák, Libor (advisor)
This diploma thesis deals with the biodegradation of whey on selected microbial products (carbohydrates, lactic acid, acetic acid and ethanol) thermophilic bacteria of genus Thermus aquaticus and mesophilic bacteria of genus Lactobacillus casei and Bacillus coagulans. For cultivation was used as medium whey, from which the proteins were removed and which was enriched with nutrients. On the basis of culture in the fermentor were determined growth curve and the HPLC method were determined individual bioremediation products.
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Study of Whey Biodegradation by thermophilic Bacteria
Fischerová, Lenka ; Vránová, Dana (referee) ; Babák, Libor (advisor)
This thesis deals with the possibilities of biodegradation of whey by the means of a mixed thermophilic aerobic bacterial culture of the Bacillus and Thermus genera. After protein had been removed, the whey was used as a medium for cultivation of the thermophilic microorganisms. The cultivations took their course in a fermentation unit and in the Erlenmayer flasks in a heated shaker in a whey medium. The bioengineering characteristics of the cultivation processes and the degree of biodegradation of the whey were evaluated. Scale of the whey biodegration was judged through the analytical characteristics – concentration of biomass, laktose and a chemical oxygen demand (COD). A decrease of CHSK was detected in all cultivations. Maximal reduction of CHSK was happend always in c. first half of each exponential phase growt, i.e. about 15 ± 3 % after first growth phase and sumarily about 62 ± 4 % after second growth phase.
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Application of thermophilic Culture for Organic Compounds Biodegradation
Piechová, Jana ; Burdychová, Radka (referee) ; Babák, Libor (advisor)
The aim of this work is focused on biodegradability study of modified polyurethane materials (foams and foils) in glukose medium and biodegradation of whey. The biodegradability was tested with using mixed thermophilic aerobic bacterial culture of the Bacillus and Thermus genera. The cultivations were practised in the fermentor and in Erlenmayer flasks in a heated shaker. Maximal reduction of COD and culture growth has been achieved by biodegradation of polyuretane in the fermentor. Main effect of degradation was abiotic destruction of PUR materials and consequent utilization of degradation products by bacterial culture. These tests indicated that the best modification agent for biodegradation was 10% carboxymethyl cellulose. The polyurethane foam was more decomposed then the polyurethane foil. Biodegradation of whey without proteins in the fermentor was detected decrease of CHSK and lactose concentration about 50 % in 20th hour. Optimal conditions for whey biodegradation with the thermophilic bacterial culture were pH 6,5 and temperature 60 °C.
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Monitoring of thermophilic organism in evaporation systems
VÁLKOVÁ, Michaela
Study deals with the determination of thermophilic and heat-resistant microorganisms in samples of skim milk. Furthermore, the problems of determining the thermophilic microorganisms in samples of whey. The most important microorganisms causing spoilage of milk and dairy products include certain species of the genera Bacillus and Clostridium. Thermophilic microorganisms have optimum growth temperature between 55 and 60 ° C. Removed material (skimmed milk and whey) was plated onto agar GTK and cultured in an incubator for 3 days at 55 ° C. Heat resistant microorganisms are characterized by resistance to heat. Prior to inoculation was necessary to inactivate other microorganisms by pasteurizing milk (85 ° C, 10 minutes). The inoculated Petri's dishes were cultured for 3 days at 55 ° C. Analysing removed material, it was found that the number of thermophilic micro-organisms in samples of skim milk does not exceed an internal limit the value of 3000 colonies per 1 ml or a gram of product (value determined Southbohemian dairy Madeta a.s.). The number of heat-resistant microorganisms has not exceeded the limit value of 2000 micro-organisms per 1 milliliter or gram. The resulting numbers of thermophilic microorganisms in samples of whey were almost identical to the results provided by Madeta a.s. factory Plana nad Luznici. The results show that after the introduction of new sanitary measures numbers of thermophilic and heat-resistant microorganisms fell sharply.
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Využití bioaktivních látek k prodloužení údržnosti mléčných výrobků
Šafářová, Iveta
The theme of this thesis is The use of bioactive compounds in order to prolong durability of dairy products. In the theoretical part, the importance, presence and influence of bioactive compounds are discussed. Also described are the individual dairy products, the use of fermentation in order to prolong durability of products and the use of lactic acid bacteria during process of production of fermented dairy products. Subsequently, the discussion focuses on the influence of bioactive compounds in dairy products on personal health. The aim of the practical part is to examine the influence of adding herbal essential oils and infusions into the sweet whey. The investigation concentrated on whether there was any influence on the chemical properties of whey and its durability.
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Výkrm prasat s využitím tradičních komponent v krmné dávce
Kořínek, Matěj
The essence of this thesis is to propose several variety's of traditional components of feed ratios in relation fattening pigs, such as sugar beet pulp, pomace, potato pulp, whey and peas. An Overall look at specification for fattening pigs is an organization of breeding, production orientation, feeding and housing. It's showing further more characteristics of traditional components, classified in feed rations and the procedure for establishing ration. The last part is formed by several variants of rations with traditional feeds included.
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Technologický postup výroby hnědého syrovátkového sýru a sledování vlivu teploty na konzistenci
Boucník, Miroslav
The content of this thesis was discussion of the technological process of production of brown whey cheese and monitoring the impact of temperature on consistency. In the production of cheese whey was used goat farm Šošůvka. Whey is a dilute solution of lactose, proteins, fat, salt, and water-soluble vitamins. The main component of the whey lactose, which constitutes 70 -- 80% of the total solids. Another major component of whey is whey protein, which are a natural part of milk. The product of evaporation of whey cheese is called Mysost or Geitost from cow and goat whey in a certain volume ratio. The finished cheese looks like a bar of soap is a semi -- hard caramel colored
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Mikrobiologie syrovátky
Štěrbová, Lucie
In its theoretical part, the bachelor thesis deals with the whey, microorganisms and their metabolic products which negatively affect human health. The procedures for reducing or complete elimination of the risk of the microorganisms are also outlined. In the practical part, the thesis determines selected groups of microorganisms present in the whey. The measurement results are presented in the thesis' summary.
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