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Biodegradation of environmental pollutants - Structural characterization of a new type of haloalkane LinB32 from Sphingobium japonicum UT26
NĚMEC, Pavel
The main aim of this thesis was focused on protein crystallography and attempt to crystallize model protein Thaumatin and a new prepared haloalkane dehalogenase LinB32 of Sphingobium japonicum UT26. Crystallization trials were performed using the sitting and hanging drop vapour diffusion methods, crystallization under oil and crystallization in the capillaries. After measuring of diffraction data, the structure of LinB32 was modeled by the use of visualizing molecular modeling programs WinCoot and VMD.

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