|
|
Growing Milk thistle (Silybum marianum L. Gaertn.) The content of biologically active substances and their use
HOFÍREK, Pavel
Milk thistle [(Silybum marianum) L. Gaertn] is among the longest known medical plants, described already in the 4th century before Christ. In the first part of my thesis, I studied the technology of cultivation this medical plant. Milk thistle contains biologically active substances. The main active substances are silybin, silydianin, isosilybin, silychristin, known as silymarin complex, and taxifolin. These substances have hepatoprotective effects on liver tissue. Characteristics, some methods of determination of active substances and pharmacological effect I deal with in the second part of my thesis. The content of active substances are influenced by growing technologies and applications elicitors. This issue and current use of plants in human medicine, dietary supplements and feed to attend in the end.
|
|
|
Electrochemistry of Flavonolignans and their Interactions with DNA and Proteins
Pyszková, M. ; Zatloukalová, M. ; Biedermann, David ; Křen, Vladimír ; Ulrichová, J. ; Ramešová, Šárka ; Sokolová, Romana ; Vacek, J.
Electrochemical oxidation of flavonolignans silybin, silydianin, silychristin and their 2,3-dehydroderivatives, was studied using ex situ and in situ cyclic and square wave voltammetry at pyrolytic graphite electrode. The pilot results presented here could be used for further investigation of mechanism of oxidation and reactivity of flavonolignans to DNA and proteins.
|
|
|
The effect of the treatment with elicitors on the content of some biologically active constituents in chosen plant
PETR, Jindřich
The aim of this thesis was to study the effect of the acetylsalicylic acid on the stimulation of plant immunity and thus the influence on the content of the active constituents in Silybum marianum L. plants. The main active constituents of Silybum marianum L. seeds are silybine, silydianine, isosilybine, silycristine, usually expressed as silymarine content, and taxifoline. These constituents have antihepatotoxic effect and many different protective effects on numerous organs and cells. Knowledge about stress and elicitation, origin, botanical characterization, growth, development and cultivation of Silybum marianum L. were summarized before the research.The small-plot experiment was set up in Hluboká nad Vltavou in 2013. Plants of Silybum marianum were divided into four groups and then three goups were treated three times during the vegetation with the acetylsalicylic acid of three different concentrations - 10-3 mol.l-1 (high), 10-4 mol.l-1 (medium) a 10-5 mol.l-1 (low). Every single one group was treated with only one concentration of the acetylsalicylic acid. The last group was treated only with water and served as a control group. The preparation of the extracts was being held with using mixture of acetone, methanol and water. The extracts were determined by High Performance Liquid Chromatography.The effects of each chosen concetration of acetylsalicylic acid (high, medium, low) on the active constituents in seeds were not statistically proven compared to seeds without aplication of the elicitor (control group). The ineffectiveness of the elicitor should have been also caused by nonoptimal condition of plants due to various abiotic and biotic stressors.
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
The effect of the treatment with elicitors on the content of some biologically active constituents in chosen plant
PETR, Jindřich
The aim of this thesis was to study the effect of two different elicitors - ELITiC and Elitic Jack on the stimulation of plant immunity and thus the influence on the content of the active constituents in Silybum marianum L. plants. The main active constituents of Silybum marianum L. seeds are silybine, silydianine, isosilybine, silycristine, usually expressed as silymarine content, and taxifoline. These constituents have antihepatotoxic effect and many different protective effects on numerous organs and cells. Knowledge about stress and elicitation, origin, botanical characterization, growth, development and cultivation of Silybum marianum L. were summarized before the research. Then the small-plot experiment was set up in Hluboká nad Vltavou in 2010. Plants of Silybum marianum were treated three times with 2,000 ml of preparation ELITiC and 2,000 ml of preparation Elitic Jack both diluted in 5 l of water. The preparation of the extracts was being held with using mixture of acetone, methanol and water. The extracts were determined by High Performance Liquid Chromatography. The effects of both preparation ELITiC and preparation Elitic Jack on the active constituents in seeds were not statistically proven compared to seeds without aplication of elicitors. The ineffectiveness of elicitors should have been caused by unsuitable chosen concentration as well as by nonoptimal condition of plants due to various abiotic and biotic stressors.
|
|
|
Cultivation Technology of Milk Thistle (Silybum Marianum)in Relation to the Product Quality and its Processing
GRAMANOVÁ, Hana
Silybum marianum has been a plant known for millenniums thanks to its pharmacological effects especially in fields of liver, gallbladder or even colon cancer treatment. A complex of effective components is called silymarin. Its amount and structure in milk thistle seeds are very important. That{\crq}s why there{\crq}s a tendency to develop methods which could increase silymarin quantity together with silymarin quality in this herb. This was also the aim of this thesis. One of the possibilities to level up the content of effective components in drugs is to bring this plant in stress. There were realized two ground-plot experiments. The stress agens was acetatonsalicylic acid (ASA) of different concentrations applicated on leaves of these plants, concretely in the concentrations of 10-5 mol.l-1, 10-4 mol.l-1, 10-3 mol.l-1. In the case of the application ASA concentration 10-3 mol.l-1 there was proved an effective action. The increase of effective components in seeds reached approximately 116,5 % ratio compared with control application of distilled water on the Silybum marianum leaves. The silymarin complex was determined by High Performance Liquid Chromatography. The preparation of the extract was practised in different ways {--} using ethyl alcohol extract and distilled water in combination with various temperatures of extracts storage. These methods were compared one another. As the best one has been turned out to be using 60% ethanol concentration for the duration of 96 hours in the storage temperature 20°C.
|
guest ::
