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Influence of humic substance HS 1500 on tolerance of Sterlet to nitrite
BULÍČEK, Vojtěch
The aim of the thesis was to assess the effect of humic substance HS 1500 on the tolerance of Sterlet (Acipenser ruthenus) to nitrite. Preparation Huminfeed was used as a source of substance HS 1500. Tolerance of Sterlet to nitrite was assessed on the basis of the results of acute toxicity tests and the results of haematological and biochemical blood examination of fish that were exposed to increased concentrations of nitrite in the presence and absence of preparation Huminfeed.
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Hematological changes in fish exposed to nitrites
FRANĚK, Roman
The aim of the thesis was to determine the effect of nitrite on hematological indices of rainbow trout (Oncorhynchus mykiss) and Nile tilapia (Oreochromis niloticus). After the fish were exposed to various concentrations of nitrites were determined 48hLC50. For the main test only fractional concentrations of these values were used. For rainbow trout it was 8 mg.l-1 NO2?, for Nile tilapia 11 mg.l-1 NO2?. The exposure of both species lasted for 48 hours. From fish blood were made blood smears to determine the influence of nitrite on size changes of erythrocytes and hematological parameters were set also. The effect of nitrite on changes in the ultrastructure of erythrocytes was determined by the electron microscopy. The erythrocyte nuclei of rainbow trout showed significant (p <0.05) shrinkage. The images of the electron microscope showed an increased amount of changes, especially in the form of various units within the cytoplasm of erythrocytes, was also visible nuclei shrinkage. There was significant (p < 0.01) reduction in the amount of hemoglobin and significant (p < 0.01) increase in the concentration of methemoglobin levels of the exposed groups. In the number of erythrocytes and hematocrit were not detected any changes. Hematological parameters of Nile tilapia did not show any size changes in the measured parameters, no changes were detected in the count of erythrocytes, hemoglobin and hematocrit values.
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Tolerance of Nile tilapia to nitrites
BROŽ, Pavel
The aim of this thesis was to evaluate the effect of increased concentrations of nitrite to Nile tilapia (Oreochromis niloticus) at different concentrations of chloride. Tilapias adults were exposed for 14 days to nitrite at the concentration of 3 mg.l?1 NO2? (i.e. concentration commonly found in recirculation systems). After 14 days the experimental fishes were divided into 4 group, which differed in concentrations of nitrite and chloride. These groups of fish were held in different conditions for the next seven days. Using standard clinical procedures the following haematologic biochemical parameters were determined: RBC, WBC, Hb, PCV, MetHb, MCV, MCH, MCHC, NO2-, GLU, Ca, Mg, TP, NH3. The tested nitrite concentration was not high enough to cause strong differences of pursued parameters. Only some of them (Hb, MetHb, MCHC) were statistically significant.
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Methods of the separation of the base flow and trends of nitrogen concentrations in this base flow
MAIEROVÁ, Monika
This Bachelor thesis is aimed to clarify the hydrological cycle, hydrological balance, groundwater hydrology, the selected basic baseflow separation methods of comparing them and last but not least, nitrogen cycle, nitrogen, nitrates, ammonia nitrogen and nitrogen in groundwater. In the work there is also closely analyzed the total, surface and subsurface outflow and the detailed division. The methods of the groundwater baseflow separation are described with regard to content of nitrogen compounds., which currently create the most common type of pollution of groundwater used as drinking sources.
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Chemical hazards of contamination of foodstuffs and dishes
MARŠÁLKOVÁ, Alena
The bachelor thesis deals with the risks of chemical contamination of food and possible health risks for the consumers. Firstly, there is a general outline of foreign substances including the additives, which are added to foods intentionally, and contaminants. The contaminants are viewed as undesirable substances which occasionally occur in foods. They develop directly in the foods or penetrate into them from the outside during the basic stages of agricultural production, in stores, during transportation or during processing for culinary purposes, or due to environmental pollution. Such substances can have negative effects on human health. The second part of the thesis focuses on concrete contaminants - nitrates, nitrites and acrylamide. Nitrates and nitrites are both contaminants and additives. Their use as food additives is motivated by their sensoric and protective effects. The sensoric function is to ensure a permanent pink colour of smoked meat and other meat products. The protective function consists in inhibiting the growth of the microorganisms Clostridium botulinum and thus preventing the origination of dangerous botulin. The nitrates exist as contaminants mainly in foods of vegetable origin, penetrating them from soil and water due to nitrogen cycle in the nature. The acrylamide is a newly discovered food contaminant. It is considered to be a very dangerous substance as regards possible peroral exposure. The danger consists in its neurotoxic, genotoxic and possible carcinogenic effects. It originates in foods with a higher content of reducing sugars during the process of production or culinary processing if the temperature is higher than 120° C. The thesis should provide information for the public as consumers or providers of different food-processing facilities. Its main objective is to pinpoint some risks connected with the consumption of various foods and meals, and to eliminate the risks as much as possible or to avoid them completely.
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Changes of biochemical parameters in fish after nitrite exposure
PODLESNÝ, Martin
The aim of this thesis was to assess the effects of long-term nitrite exposure on mortality, growth rate and blood biochemistry in rainbow trout (Oncorhynchus mykiss). Furthermore, acute toxicity tests with nitrite were performed on rainbow trout (Oncorhynchus mykiss) and sheatfish (Silurus glanis). The theoretical part focuses on aquatic toxicology including toxicity evaluation of substances and preparations for water organisms. Detailed information on nitrite are summarized there, namely information on nitrite occurrence and sources in aquatic environment and the mechanism of their uptake and toxic influence on fish including factors influencing their toxicity. The practical part consists of methodology and results of acute toxicity tests on rainbow trout and sheatfish and sub-chronic test on rainbow trout. According to the acute toxicity tests results, 96hLC50 values were estimated at 11.2 mg.l-1 NO2- for rainbow trout and 15.3 mg.l-1 NO2- for sheatfish. Sub-chronic exposure of rainbow trout to nitrite concentrations ranging from 0.01 to 3.0 mg.l-1 lasting 28 days caused an increase of glucose concentration and a decrease of potassium concentration in the blood plasma among all experimental fish compared to control. Elevated nitrite levels were found in the plasma of the fish exposed to concentrations of 0.6 mg.l-1 NO2- and greater. The plasma nitrite levels did not reach those applied in any experimental group in the present study. At highest nitrite concentration (3.0 mg.l-1 NO2-), 42 % growth inhibition and 65 % mortality among fish was noticed. On the basis of growth rate inhibition data, the values of NOEC and LOEC were estimated at 0.01 mg.l-1 and 0.2 mg.l-1 NO2-, respectively.
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