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Children with Phenylketonuria in kindergarten
BLAHOVCOVÁ, Veronika
This bachelor thesis is focussed on the problematic approach towards children with metabolic disorder ? phenylketonuria in a family and an environment of kindergarten. Congenital metabolic disorders lead to lack of certain substances in organism, or, on contrary, accumulation of them. Such disorders have various clinical displays, from harmless ones (often without symptoms) thru severe handicaps of physical and mental development to life-threatening states. The thesis is focussed on approach of parent as well as kindergartener towards child and it adverts to necessary adherence to diet restrictions.
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Effect of temperature, fish size and feeding on oxygen consumption and ammonia excretion in the Nile tilapia (Oreochromis niloticus)
DENKOVÁ, Adéla
Aim of this work were to assessed the impact of feeding, temperature, stocking density, fish size and water temperature on ammonia excretion and oxygen consumption of Nile tilapia (Oreochromis niloticus) in recirculating system. Concentration (saturation) of oxygen, water temperature and pH were measured using multimeter and concentration of ammonia was assessed by direct Nessler method. For assessing of effect of fish size, three groups of tilapias weighed 25, 65 and 140 g were established, each group in four replicates. For assessing of effect of feeding was tilapias (body weight 65g) divided into groups fed continuously, two meals per day and four meals per day, each group in four replicates. For assessing of effect of stocking density was tilapias (body weight 65g) divided into groups of 20, 50 and 80 kg.m-3, each group in four replicates. All experiments were performed for water temperature of 23, 27 and 31 °C and for fed and starved fish. Mean daily oxygen consumption was higher for smaller fish categories than for bigger ones and grow rapidly after feeding. The kind of feeding had impact on diurnal fluctuation of oxygen consumption. It was also confirmed that tilapia can survive low level of oxygen concentration for a quite long period.
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The metabolism of selected species of predatory aquatic insects
KLEINOVÁ, Miroslava
Title: Metabolism of selected species of predatory aquatic insects. Target: The main objective of this study was to analyze metabolic rates of predatory aquatic insects, in my case dragonflies (order Odonata, suborder Anisoptera). Different instars of several species of dragonflies were observed. Another objective was to determine the dependence of the metabolic demands on the species and size of the individual, create tables and graphs summarizing the most important results and perform a basic statistical analysis. Methods: In my work I used the method of measuring the consumption of dissolved oxygen, using a polarographic determination of oxygen concentration with a membrane-covered oxygen sensor. Results: The rate of metabolism on the unit weight decreased with increasing weight and size, which corresponds to the assumptions of metabolic theory of ecology.
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Impact of teperature, fish size and feeding on oxygen consumption and ammonia excretion in the african catfish (Clarias gariepinus)
KOMENDOVÁ, Jana
Aims of this thesis were to assesed the impact of feeding, temperature of water and fish size on ammonia excretion and oxygen consumption in Clarias gariepinus in recirculating system. Fish were divided in 8 weight categories from 21 to 2495 g. Experiments were performed under four temperatures: 22°C, 25°C, 28°C and 30°C. Fish were acclimatized, light conditions were: 14 hours of light period and 10 hours of dark period. Feeding was provided fed four times a day. Starved fish were measured. Measurements were held every two hours. Oxygen consumption was measured by multimeter and ammonia excretion using Nessler?s method. Average daily oxygen consumption in fed fish was dependent on temperature and average individual weight of fish, varied from 10.5 to 96.6 mg O2/kg/h and in hungry fish from 4.3 to 61.8 mg O2/kg/h. TAN excretion varied from 0 to 59.9 mg TAN/kg/h in fed fish and from 0 to 60.8 mg TAN/kg/h in hungry fish. Oxygen consumption increased with increasing temperature. Ammonia excretion was very unstable at all temperatures in all weight categories. Fish had higher ammonia excretion in light period of experiment.
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Respiration of spiders during satiation and starvation
KRAUS, Roman
I raised the young spiders breeders known species Lasiodora parahybana a stage suitable for the target work. The goal is to measure and graphically express the consumption of oxygen (O2) and record the value of spiders saturated metabolism during starvation. 37 days, I measured 20 spiders in 2 batches in Warburg respirometer. The spiders after some time has been to regulate metabolism and to the values of its viability. The resulting value is visible decrease in metabolism and weight loss.
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Blood values of sheep at a defined intake of selenium and iodine
KOCÁBOVÁ, Ivana
The theoretical part is concerned with the functional application of selenium and iodine supplementation methods, risks disproportionate to their income, including changes in biochemical and hematological parameters and evaluation methods of adequate saturation. The practical part analyzes the results of experiments on sheep with a defined content of selenium and iodine in the ration evaluation of selected indicators of the metabolic profile of ewes and their lambs. The results are processed statistically. It reflected the dynamics and interactions of monitored parameters.
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Mitochondrial energy metabolism in \kur{Trypanosoma brucei}
VERNER, Zdeněk
The thesis summarizes data gathered on various components of respiratory chain of Trypanosoma brucei. Namely, NADH:ubiquinone oxidoreductase (complex I), alternative NADH:ubiquinone oxidoreductase (NDH2) and mitochondrial glycerol-3-phosphate dehydrogenase are discussed themselves and in broader context of energy metabolism. Also, a work done using RNA interference library is described.
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A role of adipokinetic hormones in insect lipid metabolism
BÁRTŮ, Iva
This PhD. thesis summarizes the effect of adipokinetic hormones (AKHs) on a spectrum of mobilized lipids in model insect species the locust Locusta migratoria and the firebug Pyrrhocoris apterus. The results revealed that mobilization of diacylglycerols and fatty acids from the fat body into the haemolymph is not uniform and suggested there is partial specificity of individual AKHs. This could contribute to the answer of the question why some insect species have more than one AKH. The results of the thesis are supplemented by studies of AKH effect on lipid metabolism in stress conditions elicited by insecticide treatments. Co-application of AKH and insecticide enhanced the effect of insecticide
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Metabolism of B.vagans during satiation and starvation
DENKOVÁ, Adéla
The aim of the work was to introduce an extraordinary ability of Brachypelma vagans: survive some time of starvation by regulation its own metabolism to minimum. The oxygen consumption for body mass unit was measured for 576 hours for each of eleven objects. Results were placed into the tabs and graphs and were rated. During the experiment were lost four objects because of starvation and alcaly burn.
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