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The chosen validation parameters of method for assessment of aroma compounds in processed cheese analogues
Burdějová, Lenka ; Horváthová, Viera (referee) ; Vítová, Eva (advisor)
This Bachelor‘s thesis deals with the issues of method validation for assessment of aroma compounds in processed cheese analogues. The method is based on the isolation of volatiles from the sample using Solid Phase Microextraction (SPME) and their subsequent determination by gas chromatography. The theoretical part focuses on general characteristics, classification and producing technology of processed cheese analogues. Further, aroma active substances contained in cheese analogues, differences from the classic processed cheese and processed cheese analogues occurring on the Czech market are mentioned. The methods for determination of aroma active compounds with a focus on Solid Phase Microextraction and gas chromatography are briefly mentioned. Within experimental part of the work the linearity and repeatability of the SPME-GC method were verified and detection limits and limits of quantification of selected standards were determined. Then the method was applied to chosen samples of processed cheese analogues with addition of different types of vegetable fats.
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The chosen validation parameters of method for assessment of aroma compounds in elderberries
Christovová, Silvia ; Ürgeová, Eva (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the assessment of aroma active compounds in elderberries (Sambucus nigra L.). In the theoretical part the chemical composition and attributes of elderberries are described. Brief information on their healing properties is also given. The possible processing methods in food industry, as well as the traditional means of use at home, are discussed furthermore. Following chapters describe the gas chromatography method combined with the technique of solid-phase microextraction, utilized for the specification of aroma active compounds. The validation of this method is also included. The aim of the experimental part of the work was to verify a set of the selected validation parameters of the SPME-GC method, specifically linearity, the limits of detection (LOD), the limits of quantification (LOQ) and repeatability. Subsequently, the SPME-GC method was applied on the chosen elderberry juice samples. In total, 37 different volatile aroma active compounds were identified and quantified, 15 of which belonged to alcohols, 9 to aldehydes, 5 to ketones, 5 to esters and 2 other compounds. The chemical composition of individual samples was not uniform; no single sample contained all of the identified compounds.
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Determination of chlorides in pharmaceutical substances
Čutová, Michaela ; Pharm.Dr.Tomáš Schenk (referee) ; Omelková, Jiřina (advisor)
The topic of this bachelor work is a validation of the method for „Determination of chlorides in pharmaceutical substance“ is made in a form of validation of an analythical method. This titration method with potentiometric indication was developed in a company Sicor and it is used for determination of the residual chlorides volume in the substance called Pemetrexed. Validation is a process which assess suitability of a given analythic system for gaining relevant data (results). Validation constists of four tests: precision, reproducibility, lineraity and test of solution stability, although the test of reproducibility was realized in other laboratory (QC). For determnination of the chlorides was applyed titrator 716 DMS Titrino with argent combined electrode. For a data evaluation were used software tiamo 1.1 and MS Excel.
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CFD Calculations in Turbomachinery and their Validation
Váchová, J. ; Louda, P. ; Příhoda, Jaromír ; Luxa, Martin ; Šimurda, David
Numerical data obtained by 2D compressible flow CFD simulation were validated by the comparison with experimental results. The investigation was made on the mid-section of the 48" long rotor blade and it was focused on the effect of shock-wave interactions with shear layers. This interaction results in the laminar/turbulent transition where separated flow occurs and it has impacts on flow structure and the blade cascade losses. Numerical simulations were carried out by two different codes: the commercial code NUMECA based on the gamma-Re transition model connected with the SST k-epsilon/k-omega turbulence model and in-house code of the Dept. of Technical mathematics, CTU Prague/ Institute of Thermodynamics in Prague. Simulations of the laminar and the fully turbulent flows were accomplished for comparison using the same numerical approach.
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Bean Validation pro JAXB
Valový, Marcel ; Pecinovský, Rudolf (advisor) ; Šlajchrt, Zbyněk (referee)
Currently, there is no solution providing automatic validation of objects in the problem of solving Object-to-XML Impedance Mismatch. The author chose Java SE specification JAXB for Object-to-XML mapping and Java EE specification Bean Validation for validation of JavaBean objects. This thesis focuses on the interconnection of the two specifications and creation of a new specification Bean Validation in JAXB providing automatic validation at the object level during the process of marshalling and unmarshalling. This specification also provides means for mapping XML Restrictions and Facets to Bean Validation constraints. In this thesis author presents the design of Bean Validation in JAXB facility specification, its reference implementation, written by author, and users and programmers guide.
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Establishment of method for determination of selected triazine pesticides in water using GC-MS/MS
OPEKAR, Jan
This bachelor thesis deals with the determination of triazine pesticides in water using GC-MS/MS. Firstly, the development and optimisation of an analytical method was performed. The following parameters of the analytical method were chosen for optimisation: carrier gas flow rate, temperature gradient, selection of SRM transitions and collision energy of SRM transitions. Then, several extraction parametres such as SPE column drying time, selection of elution solvent, sample pH and sample volume were optimised. Finally, the method was successfully validated and applied to the analysis of a real tap water sample.
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New concepts in the care of the elderly: Validation by Naomi Feil, Psychobiography model by prof. Böhm, Sensory activation by Lohre Wehner
KOUBOVÁ, Gabriela
This bachelor´s work is theoretical and the aim is to describe new models and their relations to the clients and employees at the seniors´rest homes on the base of special literature and of experience from the professional schooling. The work contains the discussion comparing these models. By way of introduction I define the expressions of old age, aging process and their displays. Then I focus on description of seniors´ rest homes, on the related standards and on the employees of the helping professions. The last chapter is devoted to new conceptions of seniors´ care, which are e.g. Validations by Naomi Feil, Psychographical model by professor Böhm and Sensory activation by Lohre Wehner. This work means the help to understand these models and to show how to ease and improve the quality if clients´ lives. It could be very helpful for those who take care of the senior clients and who try to understand them.
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Verification of glucose assessment analytical method
ILLEOVÁ, Kateřina
The topic of laboratory accreditation as well as validation and verification of analytical methods has been very current in recent years. Because of the serious impact of analytical results of the measurement on practice, it is required that the results of the analytical measurements were as accurate as possible. This paper deals with the verification of the analytical method determination of glucose, as proof of achieving optimum measurement uncertainties. The verification of spectrofotometric glucose determination was performed using the Cobas Integra 800 analyzer by enzymatic hexokinase method. For the measurement in time the control materials of internal quality control were used and for the measurement in series the control materials of external quality control were used. From the measured values we specified the necessary statistical data (the arithmetic mean, standard deviation, the coefficient of variation, bias, yield) and using these we specified the values of combined and extended uncertainty. The combined uncertainties of both samples shouldn't be higher than 10 %, according to the SEKK association. The combined uncertainty of our first sample was 6.81 % and the second one had 6.45 %. The extended uncertainty of the first sample was 13.62 % and the second one had 12.90 %. From these data, we can conclude that the verification of analytical methods for the determination of glucose succeeded.
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Validation of macromolecular crystal structures
Řezáčová, Pavlína
X-ray crystallography is the main technique of macromolecular structure determination. Typical problems of protein crystallography diffraction data measured at limited resolution and inaccurate phase informacion adversely affect the quality of electron density maps and consequently the quality of the final model. Currently, there are many methods of protein quality validation. These can be subdivided into two main groups The first group assesses the model quality by evaluating the agreement of the model with the diffraction data. The second group of structure validation methods is based on comparison of the macromolecular model geometrical parameters with standards values derived from the crystal structures of small molecules or high-resolution macromolecular structures. This work is focused on introduction to widely used macromolecular structure-validation software. The main global quality criteria and reliable indicators for detecting local errors are summarized.
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