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Crystallographic study of the iron-regulated outer membrane lipoprotein (FrpD) from Neisseria meningitidis
SVIRIDOVA, Ekaterina
Neisseria meningitidis (N. meningitidis) is a Gram-negative commensal bacterium colonizing nasopharynx of about 10 % of healthy individuals, which can cause invasive diseases, such sepsis and meningitis, upon occasional penetration into bloodstream. Pathogenesis of N. meningitidis appears to be directly related to conditions of limited iron availability. Under these conditions two proteins of unknown function: FrpC and FrpD, are synthesized. FrpD is a highly conserved lipoprotein of N. meningitidis anchored to the bacterial outer membrane. It is known that FrpD tightly binds the FrpC protein, which belongs to the Repeat-in-Toxin (RTX) protein family and may act as bacterial exotoxin. However, the mechanism of FrpD-FrpC interaction and the exact function of this complex are unknown due to the absence of structural information on these proteins. Therefore, we set out to determine the structure of FrpD and provide insights into its interaction mechanism with FrpC and structure-functional relationships of these two proteins. We determined the first crystal and solution structures of the FrpD protein. We found that atomic structures of FrpD reveal a novel protein fold. We uncovered the structure-function relationships underlying the mechanism of interaction between the FrpD and FrpC proteins and tested the putative function of the FrpD-FrpC1-414 complex in vitro. Finally, we proposed the putative function of the FrpD-FrpC1-414 complex as a new minor adhesin of N. meningitidis, which mediates the bacterial adhesion to the host epithelial cells and facilitate the colonization. Our work constitutes the first step in clarifying the molecular basis of the FrpD-FrpC interaction and sets the base for further investigation of the role of FrpD and FrpC in the virulence mechanism of N. meningitidis.
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The development of the crime on credits in the South Bohemia before 2014
BUŠKOVÁ, Tereza
The aim of this study is to analyze the development of committing a credit fraud offence under § 211 of the Act. no. 40/2009 Coll., the Penal Code (particularly the development of this crime and the development of the amount of the damage) and consequent comparing the selected macroeconomic indicators development in the period 2005-2014 in the region of South Bohemia. In this work secondary data analysis methods were used. Statistical data obtained from the South Bohemian branch of the Czech Statistical Office and statistical data from Crime Police of the Czech Republic was used as the source. The data evaluating the amount of detected cases of this crime committed by the offenders in the Region South Bohemia, according to the region where the fraud was commited, was analyzed. The analyzed file was 5,713 offenses and 4,219 offenders. The defined set was divided into seven parts (districts), according to the local jurisdiction of the territorial departments of the Czech Police. The observed data was compared with selected macroeconomic indicators. From the results it can be concluded that the highest rate of loan fraud offence (calculated per 1,000 inhabitants) is in the district Stra-konice. Conversely, the lowest crime in the same period is in the district Jindřichův Hradec. This work also re-vealed that the development of macroeconomic indicators has no effect on the development of this crime. In conclusion, it is recommended to promote economic education at primary and secondary schools, thus improving the financial literacy of the population. Furthermore, we can recommend the consistent use of, the Central Credit Register, which is already available, as well as supporting of changes in the legislation, so that the providers have an obligation to investigate the credit applicants more and make the offenders of this crime discouraged by higher penalties imposed for credit fraud.
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Role of the nurse in screening for depression in the elderly.
VRZALOVÁ, Monika
The diploma thesis deals with problems of depression in older people. Mainly the work is focused on identifying and analyzing the role of nurses in screening for depression in older people in primary care, acute care, long-term care and home care. This thesis was focused on theoretical direction and was used the method of design and demonstration. In this thesis was set one main goals with five research questions. The main goal was to identify and analyze the role of nurses in screening for depression in the elderly. RQ 1: What is the role of the nurse in screening for depression in the elderly? RQ 2: What is the role of the nurse in the primary care in screening for depression in the elderly? RQ 3: What is the role of the nurse in screening for depression in hospitalized patients in acute care? RQ 4: What is the role of the nurse in screening for depression in seniors in long-term and home care? RQ 5: What rating scales and methods are used in screening for depression in the elderly? The thesis introduce the concept of depression. The following are specified the causes of and the important factors that affect depression in the elderly. It also deals the differences in the clinical symptomatology of depression in old age. It explains possibilities and various barriers in the diagnosis of depression. Another chapter introduces complete geriatric examination, diagnostic classification systems, possible screening methods and scales for detection of depression in the elderly population. It also deals methods of pharmacological and non-pharmacological treatment and its possible complications associated with older age. By reason of increased suicide rate caused by depressive disorder the issue of suicidal behavior in the elderly is introduced. The next chapter deals with the nursing process, which is used by nurses in practice. It consists of the evaluation of the patient's health condition, making nursing diagnosis, creating nursing plan and subsequent implementation and evaluation. The nursing process is also needy for providing quality care. The nursing process in the stage of nursing diagnosis, introduces possible nursing diagnosis for a patient suffering from depression, which are based on the latest classification. Finally is described the role of nurses in screening for depression in the elderly in different health facilities and their contribution to the timely evaluation of depression in the elderly. This chapter introduces the role of nurses, nursing screening and collaboration with a physician. The role of nurses in screening for depression in different medical facilities is based on the first phase of the nursing process of assessment. On the basis of objective and subjective information, the nurse will assess the overall health and mental condition of the patient. Primarily, it was investigated what is the role of the nurse in screening for depression. On the basis of content analysis and synthesis it was necessary to used and processed domestic and foreign literature. A number of relevant sources are the results of various studies and Meta-analyzes, mostly from abroad, but also from the Czech Republic. The thesis can serve as a basis for nurses. The result of this thesis is to create e-learning material available for students in the Faculty of Health and Social Sciences of South Bohemia in Ceske Budejovice in the tutorial called Moodle.
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Vliv teploty vody a intenzity světla na raný vývoj sumečka afrického v komerčním chovu
PROKEŠOVÁ, Markéta
The successful production of quality early fish stages is crucial for artificial rearing fish species. Nevertheless, it is quite a problematic process, because fish as poikilothermic organisms are highly sensitive to various environmental conditions. Moreover, both fish embryos and larvae are more affected by biotic and abiotic factors (such as predation, stocking density, temperature, light, pH, salinity, oxygen saturation, feeding availability, or their interactions) than older juveniles or adults. Most fish processes such as reproduction, metabolism, survival, development and growth can be considerably (positively, negatively or not) influenced by these conditions. Therefore, it is important to understand their impacts on fish stages during embryonic, larval, juvenile, adult, and eventually the senescent period. Then, following the application of new knowledge to the practise, especially species- and age-specific optimal conditions, should result in higher survival, better growth rate, well developed fish without deformities and lower cannibalism. The objectives of the present thesis were to assess the effect of water temperature and light intensity on the early development (embryonic and larval period) of African sharptooth catfish as a perspective fish species for freshwater intensive aquaculture. Due to its great characteristics (for instance fast growth, ability to breathe atmospheric air, rearing at high stocking density) this species can meaningfully contribute to the global aquaculture production which is expected to rise in the coming years. However, there are still some difficulties in the early rearing African sharptooth catfish regarding the cannibalism, feeding, survival and necessity of detailed information about the effects of some abiotic factors and their interactions.
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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VÝVOJ MIKROSATELITŮ U NEMODELOVÝCH DRUHŮ ŽIVOČICHŮ
ZIMA, Jan
This thesis is a compilation of six publications describing development of microsatellite markers for four mammal species and five insect species. Most of these species are endangered and the aim of developing microsatellites was their use in conservation-genetic studies. The thesis thus represents a contribution to methodological tools for investigation of endangered animals. Different approaches were used for the development of the loci, including inovative pyrosequencing, which represents a trend in DNA analyses with variety of practical aplications. Besides other things, this thesis documents increasing availability of the most modern molecular biology methods and their utilization in classical biological disciplines such as zoology, botany and ecology.
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Transboundary eel (Anguilla anguilla) management plans
Lehoučková, Sabina ; Kalous, Lukáš (advisor) ; Nechanská, Denisa (referee)
In the bachelor thesis are collected and compared data obtained from available materials that deal with the issue of declining state of European eel (Anguilla anguilla) in European waters. Population decline was recorded already in the 80s of last century and throughout the range, which includes most inland and coastal waters of Europe and North Africa. The first chapter describes the species of Anguilla anguilla, developmental stages and migration cycle, who during his life this species pass. In the following part of the work is information on the Council Regulation (EC) No. 1100/2007 of 18 September 2007 establishing measures for the recovery of the stock of European eel. This regulation requires the individual Member States of the European Union to draw up plans Management of European eel on their territory if the area falls between habitats eel. The next section discusses the management plans for the Czech Republic, England and Wales, the Netherlands, Denmark and Spain. The management plans are then selected individual chapters devoted to the definition of the basin, which measures for the recovery of the eel population concerning the procedure for determining the target escapement levels of 40 % of adults with eel in marine waters and further concrete measures implemented by means of the watercourses which should be the target level achieved. The final part is devoted to the discussion, which are summarized and compared the measures used by Member States in the individual management plans. Furthermore, this part contains data on the eel population after the introduction of primary measures for its renewal.
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Revitalization of the chateau park in Choltice
Čermáková, Michaela ; Vaněk, Jan (advisor) ; František, František (referee)
This work has the task to assess the current situation of the chateau park and at the same time the game preserve in Choltice, and suggest its revitalization in order to rejuvenate the vegetation and making the field available for the general public. Choltice is a market town in the Pardubice region, approximately 15 km west from Pardubice. At the end of 17. century a baroque castle was built here, which belonged to the count Simon Thun. His family belonged it until 1945. The chateau and the park is now owned by the borough Choltice. The chateau neighbours with the extensive English park, which passes the chateau game preserve, which is since 1992 a nature reserve and an important European locality.
The diploma thesis contains a detailed theoretical basis and proposes solutions, which are directed both on the protection of the living conditions of protected and endangered species of a beetle Osmoderma eremita. This species occurs only rarely and his survival area is very limited, therefore it is necessary to create those conditions, and it is currently one of the main tasks of the revitalization.
The next step is to help revitalize the introduction of spontaneous breeding fallow deer in the game preserve. This idea is not new, the fallow deer were bred there until 1945. The town of Choltice is very inclined towards this idea about farming and supports it. To increase the number of visitors this diploma thesis contains a proposal for the creation of a nature trail, this area would attract wider public than so far.
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Archaeological findings Avar-Slavic girdle fitting in the Czech Republic
VARAUSOVÁ, Michaela
The subject of this thesis is processing found Avar-Slavic belt fitting the territory of the Czech Republic. One of the most important aims of this thesis is the develop a typology of belt fitting and subsequent broken down into tables. The next aim is to provide a basic overview and informations about found tip of the Czech Republic. However, attention was paid to typology of the part of a horse harness. One part of this thesis is catalog of found belt fitting too, which was created of the basis of informations of the available literature.
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Potential of sustainable development of the Village
Uhlíková, Ivana ; Varvažovská, Pavla (advisor) ; Marcela, Marcela (referee)
This thesis analyzes the sustainable development strategy focusing on the environmental pillar and the subsequent evaluation of the implementation of environmental sustainability in Kladno. The main aim was to evaluate the active and environmental policy perspective of the city.
In the theoretical part are described various laws, documents and instruments relating to environmental policy and sustainable development. The thesis is talking about the area of interest, its historical development, demography, environment, social and cultural fields.
Using concrete examples it is recommended how the environmental management of Kladno could proceed in the future. The proposed recommendations are a result of analysis of all available information which include the needs and requirements of city residents.It is important to update Strategy of environmental policy and introduction of activities from the Sustainable Development Strategy SO ORP Kladno in to practise. Improving of public services and community cohesion. Establishment of school forums in primary schools.
The final part contains the results of a field survey. Questionnaires were handed and collected in combination with controlled interviews.
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