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Methodology of highly reliable systems design
Straka, Martin ; Gramatová, Elena (referee) ; Racek, Stanislav (referee) ; Kotásek, Zdeněk (advisor)
In the thesis, a methodology alternative to existing methods of digital systems design with increased dependability implemented into FPGA is presented, new features which can be used in the implementation and testing of these systems are demonstrated. The research is based on the use of FPGA partial dynamic reconfiguration for the design of fault tolerant systems. In these applications, the partial dynamic reconfiguration can be used as a mechanism to correct the fault and recover the system after the fault occurrence. First, the general principles of diagnostics, testing and digital systems dependability are presented including a brief description of FPGA components and their architectures. Next, a survey of currently used methods and techniques used for the design and implementation of fault tolerant systems into FPGA is described, especially the methods used for fault detection and localization, their correction, together with the principles of evaluating fault tolerant systems design quality. The most important part of the thesis is seen in the description of the design methodology, implementation and testing of fault tolerant systems implemented into FPGAs which uses SRAMs as the configuration memory. First, the methodology of developing and automated checker components design for digital systems and communication protocols is presented. Then, a reference architecture of a dependable system implemented into FPGA is demonstrated including several fault tolerant architectures based on the use of partial dynamic reconfiguration as the mechanism of fault correction and the recovery from it. The principles of controlling the reconfiguration process are described together with the description of the test platform which allows to test and verify the design of fault tolerant systems based on the methodology presented in the thesis. The experimental results and the contribution of the thesis are discussed in the conclusions.
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Methodology of highly reliable systems design
Straka, Martin ; Kotásek, Zdeněk (advisor)
In the thesis, a methodology alternative to existing methods of digital systems design with increased dependability implemented into FPGA is presented, new features which can be used in the implementation and testing of these systems are demonstrated. The research is based on the use of FPGA partial dynamic reconfiguration for the design of fault tolerant systems. In these applications, the partial dynamic reconfiguration can be used as a mechanism to correct the fault and recover the system after the fault occurrence. First, the general principles of diagnostics, testing and digital systems dependability are presented including a brief description of FPGA components and their architectures. Next, a survey of currently used methods and techniques used for the design and implementation of fault tolerant systems into FPGA is described, especially the methods used for fault detection and localization, their correction, together with the principles of evaluating fault tolerant systems design quality. The most important part of the thesis is seen in the description of the design methodology, implementation and testing of fault tolerant systems implemented into FPGAs which uses SRAMs as the configuration memory. First, the methodology of developing and automated checker components design for digital systems and communication protocols is presented. Then, a reference architecture of a dependable system implemented into FPGA is demonstrated including several fault tolerant architectures based on the use of partial dynamic reconfiguration as the mechanism of fault correction and the recovery from it. The principles of controlling the reconfiguration process are described together with the description of the test platform which allows to test and verify the design of fault tolerant systems based on the methodology presented in the thesis. The experimental results and the contribution of the thesis are discussed in the conclusions.
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The Importance of the Fundraiser in a Non-profit Organization
STARÁ, Radka
The main theme of the bachelor work is the importance of the fundraiser in a non-profit organization. It defines the term fundraising, its principles, forms and methods, the work of a fundraiser in a non-profit organization.The work characterizes nonprofit organizations, their legal status and field of activity. There are also information about activities, which improve economy and financial situation of these organizations. In the practical part of my work I have analyzed the way of financing a non-profit organization Junak- Czech scout, Centre Pod Kleti Holubov z.s. I am comparing in this part the way of fundraiser work on the local and state platform. The facts here available are drawn from the public web pages of this organization and also from the account keeping documents of its local center. At the conclusion of my work I am assessing the effectiveness of fund raising plan and its strategy and also describing the way of negotiation with donors. I am noticing the need of a fund raising team creation and also rules of fundraising including necessary cooperation between the center of organization and its local branches.
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Political competition in local governments and public procurement
Palguta, Ján
Municipalities in the Czech Republic manage 23.9% of the total public expenditure (approximately 10% of the GDP), with approximately half of these funds redistributed through public procurement. This is a very significant amount of public resources that should be given due attention. This analysis demonstrates that the composition of municipal governments significantly influences the process of public procurement. Our methodology allows to interpreting the estimated relationships as causal, rather than as mere correlations.
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Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head
Šebková, Nataša ; Frolíková, Michaela ; Děd, Lukáš ; Dvořáková-Hortová, Kateřina
CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction (AR). It also associates through membrane integrins with specific MAP kinases involved in the AR. Our aim was to monitor the dynamics of relocation of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. The dependence of this localization changes on the dynamic of actin cytoskeleton was studied. Our results show the changes in the localization of these proteins associated with the AR and their co-localization was observed using proximity ligation assay. After the AR CD46 and β1 integrin spreads across the sperm head, entering the post-acrosomal compartment, and permeates the borders of different domains. It was shown previously that actin dynamics is necessary for acrosome reaction-associated translocation of Izumo1 protein that is required for sperm-egg fusion. Therefore Latrunculin A was used during sperm incubation. The co-incubation of capacitated sperm with Latrunculin A leads to a decrease of the percentages of sperm, which express relocation pattern after induced AR. 3D models and visualizations of potential membrane processes responsible for the relocation of proteins from the acrosomal area to the other compartments of the sperm head were prepared. Our results deliver new information that proteins CD46 and β1 integrin undergo dynamic relocation towards the sites of sperm-egg fusion during the AR in vitro. The inhibitor of actin dynamics abrogates significantly the AR-associated changes in proteins localization. We speculate that this relocation is of importance for the successful sperm-egg interaction, adhesion and subsequent gamete fusion.
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Dynamics of mouse sperm capacitation and acrosome reaction
Dvořáková-Hortová, Kateřina ; Frolíková, Michaela ; Děd, Lukáš ; Šebková, Nataša
Capacitation followed by the acrosome reaction (AR), is a very complex event of molecular changes, including acrosome matrix rearrangement and actin polymerization, which mammalian sperm must undergo in the female reproductive tract in order to obtain the ability to penetrate and fertilize the egg. CD46 and β1-integrin belong to specific proteins, which are predicted to interact during molecular reorganization of capacitating sperm. The IZUMO1 as the primary fusion protein of the mammalian sperm is also involved in this dynamic network. We investigated the relationship between the Izumo, CD46 and β1 integrin relocation in the sperm head during the capacitation and AR in vitro. We have already successfully monitored by immunofluorescent labelling the dynamics of proteins CD46 and β1-integrin. The changes in the localization of these proteins associated with the AR and their mutual co-localization was observed. The original β1-integrin location in the freshly released epididymal sperm is in the acrosome and it relocates during the AR further through the sperm head compartments into the equatorial segment and over the whole sperm head. Its density over the equatorial segment is decreasing with the extended time of the AR. Also its presence in the perforatorium of the mouse sperm head is very prominent. The pattern for protein CD46 is extremely similar if not identical in both aspects such as compartment localization and time progress during capacitation and AR in vitro. The molecular interaction of CD46 and β1-integrin was investigated using the Proximity Ligation Assay and Super resolution microscopy STED. The data were statistically analysed. The newly obtained results from CD46 and β1-integrin relocation are in correlation with IZUMO1 dynamics and giving a substantial knowledge on the studied protein network rearrangement during capacitation and AR in mouse spermatozoa.
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Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.
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Fish population on the river Dyje at NP Podyjí
Štrunc, David ; Kalous, Lukáš (advisor) ; Kuříková, Pavlína (referee)
This thesis is focused on changes of fish populations in the river Dyje located at the national park Podyjí. These changes are caused mostly due to presence of two large dams (Znojemská and Vranovská dam) between which w the study locality is situated. There are also other factors affecting ichtyofauna composition as piscivorous predators, non-native species or restocking of particular target species. First information about the fish populations appeared in year 1921. In that years, the locality contained species, that are not present at the river site at the moment. Examples given: sturgeon (Acipenser ruthenus) and danube salmon (Hucho hucho). These species extincions were associated with interupted fish migration between locality and Donau river basin. On the other hand, new fish pecies (that are not-native to Czech Republic and were not observed before 1921) started to appear during following years. From the former fish population of type Barbus-Chondrostoma the type changed to Salmo-Thymallus. Most of this change was caused by regular restocking of the salmonids. Since 1993 there is evidence on new presence of tolerant fish species that do not necesarilly need cold, flowing water. There is high probability, that this appearance is caused by slowing down the river stream and also the heating of the water, that takes place in the dam.
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New Nature Trail on Dendrology in the Podyjí National Park and the Integration of the Trail into the Existing Trail Network
Šlechtová, Hana ; Kuneš, Ivan (advisor)
This thesis deals with the creation dendrological nature trails and its integration into the existing network of hiking trails. The proposed route of the nature trail was selected the National Park Podyjí and location Lesná - Ice caves.
Work is also engaged in the exploration habitats and trees in this area. According to their location in the terrain along the trail, they were scheduled individual stops. The topic itself stopping is a graphic and text attachments to individual tree species and their habitats within the meaning of National Park. They were made graphic designs of information boards. These are presented as separate outputs nature trails.
The thesis deals with research already existing network of hiking trails in the localized area. It offers the possibility of tourists use the proposed nature trails in the overall assessment of attractive tourist destinations National Park. On the territory of the National Park were surveyed exploration existing nature trails. The inquiry was the issue of the length and design information boards.
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Abundance of house sparrow (Passer domesticus) and other synantropic species in small settlements around Prague
Řezáčová, Andrea ; Šmejdová, Lucie (advisor) ; Zasadil, Petr (referee)
In the surroundings of Prague, as well as in many other parts of Europe the population numbers of synanthropic species were reduced in recent years. These changes in abundance are attributed to the change of use of agricultural landscapes, the loss of small poultry farms and mainly a change in the character of the construction. The aim of this thesis was to ascertain the abundance of selected species of synanthropic birds and compared their numbers with the data obtained in 2012. In this study were also evaluated differences in the abundance of these species in the localities of old construction area and new construction area. There were selected 3 main leaders, house sparrow (Passer domesticus), tree sparrow (Passer montanus) and eurasian collared dove (Streptopelia decaocto) from all monitored species for the statistical processing of the results. Census was carried out in a total of 40 squares, 20 of which was located in an old construction area and 20 in the construction of the new. All of the site lies in an area with a high degree of suburbanization in the central region an in around of Prague. The study
area covered an area of 200 x 200 meters and were visited a total of twice during the nesting period in the spring of 2015. The results show the preferences of the old buildings for the house sparrow and the eurasian collared dove. Tree sparrow was more numerous in the localities, characterizing the new housing development. For eurasion collared dove was observed increasing trend in abundance, while in both species of sparrows was recorded its decline. The most numerous species was the house sparrow with a total of 209 individuals, on the contrary, at least individuals, 23, was noted for european serin (Serinus serinus).
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