|
|
Determination of the nutritional properties of paprika of different geographic origin
Kovaříková, Tereza ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
This master thesis deals with the analysis of nutritional properties of paprika condiment with different geographic origin. The theoretical part contains botanical description of red pepper (Capsicum annuum L.), its chemical composition, process of production of paprika and then they are described analytical methods suitable for determination of fundamental nutrional properties. Experimental part is focused on the preparation of samples and their analysis. In total, 11 samples were selected for the experiment, 5 of them had a protected designation of origin. These samples were from Slovakia, Hungary, Bulgary, Romania, Turkey and Spain. The Soxhlet extraction was used for the analysis of the fat content, Kjeldahl method was used to determine gross protein. High-performance liquid chromatography (HPLC) was chosen to determine carbohydrates and capsaicin. Induction-coupled plasma emission spectroscopy (ICP-OES) was used for elemental analysis. The results were compared with each other and with available databases and literature. For comparing and finding certain connections, the principal component analysis (PCA) was selected.
|
|
|
Detekce mikrobiální kontaminace molekulárně-biologickými metodami v kořeninové paprice
Machálková, Zuzana
Dryed pepper is one of the most used spice in many parts of the world. The presence of undesirable pathogens can be a serious problem for consumers. Especially contamination by microscopic fungi, some of which produce mycotoxins representatives, poses a great threat to humans. Today's conventional method for determining contamination by microorganisms are time consuming and some less common types of pathogens can be difficult to determine. In the context of this thesis were applied molecular biology methods for detection of contamination in samples of pepper. There were selected ITS regions for sequencing, which show high variability between species and organisms are an appropriate tool for the taxonomy. The sequence analysis of the ITS regions were identified by sequence high similarity with the ITS regions of many microscopic fungi especially representatives of the class of Ascomycotina and several yeast species. The work was a qualitative rather than a quantitative detection, which does not exclude the wholesomeness of the samples, which is proven by health protocols.
|
|
| |
|
| |
|
| |
|
| |
guest ::
