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Changes in the content of gliadin protein fractions in two genotypes of winter wheat with different lengths of vegetation time depending on nitrogen fertilization with drought interaction.
Francová, Marie ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the influence of the nitrogen fertilization and drought on the change in the content of gliadin protein fractions in two genotypes of winter wheat Avenue and Tobac was studied. These two genotypes differ in vegetation time length. Half of the plants were fertilized using nitrogen fertilizer at 200 kg N/ha. One third of the plants were cultured under the conditions of early drought (in bloom season), other one third was grown under the conditions of of late drought (grain filling season), and last third was grown under the conditions of natural irrigation. Individual gliadin fractions were separated by using A-PAGE method and their content quantified by computer densitometry. Our results have shown increase in gliadin fractions content after nitrogen fertilization. Early drought itself caused significant increase in the levels of -gliadin fractions in Tobac genotype. Early and late drought in combination with nitrogen fertilization increased levels of gliadin fractions in Tobac genotype. Early drought in combination with nitrogen fertilization had no effect on Avenue genotype, except of -gliadin fractions which decreased significantly. Late drought in combination with nitrogen fertilization caused significant increase in gliadin content in Avenue genotype. The highest increase in gliadin content was observed in fraction -5 of the Tobac variety during interaction nitrogen fertilization with late drought.
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PĚSTOVÁNÍ PŠENICE V SETRVALÉM ZEMĚDĚLSTVÍ
PETÁKOVÁ, Šárka
The aims of the bachelor study titled Wheat Production in Sustainable Agriculture were to compare wheat production levels of organic and conventionally managed fields, namely, to assess the influence of the fertilization regime on the formation of yield elements and also, to assess the overall yield ability of selected winter wheat varieties. A small field with winter wheat varieties located on the university grounds was used as an experimental site for this study. The site is located in Ceske Budejovice in South Bohemia at an altitude of 380 m, with average annual temperature reaching 7.8 °C and with the average rainfall of 620 mm. Four varieties (two hybrids nad two lineages) were examined under three different fertilization treatments: no nitrogen fertilizer, nitrogen fertilizer and organic fertilizer (clover-based). The study has found that the choice of the variety appears to have most impact on the overal yield. Hybrid varieties outperformed lineage varieties in overal grain yield, despite the fact that lineage varieties produced higher results in regard to the following yield factors: the number of plants, offshoots, ears, grains in the ear per m2 and weight of 1000 grains. The choice of fertilization treatment seemed to influence the crops outcomes less substantially than the choice of the variety per se. While the influence of the fertilization treatment was secondary, nitrogen-fertilized plots produced the highest grain yields (7,78 t.ha-1),, organic fertilization (clover) produced average results (7,59 t.ha-1), and the the lowest yield has been predictably produced in non-fertilized site (7,05 t.ha-1).
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Impact of nitrogen fertilization and drought on glutenins content in winter wheat
Vostrejš, Pavel ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
In this bachelor thesis the effects of nitrogen fertilization and drought stress on glutenin fraction of winter wheat (Triticum aestitivum L.) were studied. Winter wheat cultivar Tiguan was grown under different rainfall mode, on the one hand in drought (DRY), on the other hand in normal downfall (WET). Part of the samples was treated with nitrogen fertilization at a dose of 140 kg / ha (N140), the other part remained without fertilization (N0). After the gliadins were separated, the glutenins from the samples were extracted with 50% isopropanol with 1% DTT. Individual glutenin fractions were separated by SDS-PAGE and quantified by computer densitometry. The drought did not have a statistically significant effect on any of the glutenin subunits. Nitrogen fertilization caused an increase in the total amount of glutenin as well as the amount of individual subunits. Interaction of water regime treatment and fertilization has not led to an increase in glutenin levels in HMW 3 GS and LMW 5 GS in DRY conditions.
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Technologie pěstování a vliv hnojení N na výnos silážní kukuřice
DOLEŽAL, Jiří
This bachelor thesis deals with the influence of nitrogen on the formation of yield components in maize. The entire work is divided into two parts, a theoretical part and practical part. In the theoretical part of the corn I characterize morphological and botanical point of view. Further described technology of growing maize. In practical part I describe the methodology and workflow. For this work were selected two maize hybrids. I fertilized this hybrids with conventional nitrogen fertilizers. In conclusion, I compare the results of yield componets both varieties and profitability of individual variants.
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Impact of nitrogen fertilization and drought on gliadins content in winter wheat
Odstrčilová, Eva ; Obruča, Stanislav (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.
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Impact of elevated carbon dioxide concentration on protein content of gliadin fraction in winter wheat
Šimíčková, Adéla ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
The aim of this bachelor thesis was to study the impact of elevated CO2 concentration, nitrogen fertilization and UV radiation on the content of gliadin fractions in winter wheat. Winter wheat (Triticum aestivum) var. Bohemia was cultivated in conditions with ambient and elevated (700 mol mol-1) CO2 concentrations. Nitrogen nutrition (200 kg N ha-1) was added to half of the samples, while the another half of samples remained unfertilized. Plants were grown in greenhouses both with shielded UV-B radiation and with normal UV radiation. Environment with natural rainfall was the same for all of these variants. Gliadins were extracted by 2-chlorethanol, separated by A-PAGE method and subsequently quantified by computer densitometry. Significant increase of gliadin proteins was noticed in conditions of nitrogen fertilization in natural UV radiation, both in elevated and ambient CO2 concentrations, whereas in an eliminated UV-B radiation significant effect of nitrogen was not observed. Therefore UV radiation influenced in combination with nitrogenous nutrition as stimulative factor. However, statistically significant impact of evelated CO2 concentration on gliadin content was not proved.
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Impact of elevated carbon dioxide concentration on protein content of gliadin fraction in winter wheat
Hamříková, Dominika ; Omelková, Jiřina (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis protein content of gliadin fractions in winter wheat (Triticum aestivum) var. Bohemia was studied. The crop was cultivated in conditions with ambient (AC) and elevated (EC; 700 mol•mol-1) carbon dioxide concentration. Moreover, half of the samples was fertilized with nitrogen in an amount of 200 kg•ha-1. Other observed environmental factors were drought and UV radiation. The gliadin proteins were separated by A PAGE method and quantified by computer densitometry. Generally the protein content within , and gliadin fractions varied, while the protein content of gliadins remained unchanged or almost unchanged. Clearly the nitrogen fertilization had the most pronounced impact on the gliadin protein content and it significantly increased the protein content in wheat grain. Most subfractions reacted in conditions of AC, drought and without UV radiation and in conditions of EC with natural rainfall and UV radiation. The interaction of nitrogen fertilization with UV radiation (AC, drought) was significant and so was the interaction of nitrogen fertilization excluding UV radiation (EC, drought). The interaction of nitrogen fertilization and natural rainfall significantly increased the protein content in conditions of AC without UV radiation and in conditions of EC with UV radiation. EC alone and the interaction of EC with other factors had only a small impact. The impact was the most pronounced in interaction with nitrogen fertilization. EC with nitrogen fertilization (drought without UV radiation) increased the gliadin protein content and EC excluding nitrogen fertilization (drought and natural rainfall with UV radiation) decreased the protein content.
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The influence of increased nutrient input on rhizospheric effect in wet meadows´ soils
BURIANOVÁ, Jitka
This master thesis deals with increased nutrient input in two wet meadow ecosystems. The study sites were Záblatské louky with organic soil and Hamr with mineral soil are both located in nature protected area Třeboňsko. This study focuses on effect of eutrophication on direct counts of bacteria, as well as Ntot and Ctot concentrations in the rhizosphere and bulk soil. Changes of all measured paremeters were observed during the growing season 2006 -2007 in both sites. Fluctuation of Norg, N-NH4 and N-NO3 concentrations occurred as well.
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