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Determination of the stoichiometry of the copper complexes with dehydrosilybine A
Klimková, Kateřina ; Mladěnka, Přemysl (advisor) ; Hrdina, Radomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Kateřina Klimková Supervisor: Assoc. Prof. Přemysl Mladěnka, Pharm. D., Ph.D. Consultant: Assoc. Prof. Kateřina Valentová, Ph.D. Title of Thesis: Determination of the stoichiometry of the copper complexes with dehydrosilybin A Silymarin, the standardized extract of the milk thistle (Silybum marianum), is a widely used approved over-the-counter drug that is recommended for a number of liver diseases. Silymarin contains as one of its components 2,3-dehydrosilybinA, which has an appropriate metal binding site in its structure. In general, flavonolignans, due to their structure, can interact with transition metals in the gastrointestinal tract by forming complexes. This property can be useful for the protection against excessive amounts of metals in the body. The aim of this in vitro study was to analyse the interaction of 2,3-dehydrosilybin A with copper, which plays a crucial role in the organism as a cofactor of many enzymes. Although being an essential element, it can, however, be toxic at elevated levels. Stoichiometry, as one of the most important characteristics of the complex, was determined by UV-Vis spectrophotometry in four (patho)physiological pH conditions (4.5; 5.5; 6.8; 7.5)...
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Aroma active compounds of selected types of herbs
Škutová, Pavla ; Hrstka, Miroslav (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with the identification of aroma active compounds in medicinal herbs. The group consists of 5 types of herbs: lemon balm (Melissa officinalis), pot marigold (Calendula officinalis), clary sage (Salvia sclarea), milk thistle (Silybum marianum), goat’s rue (Galega officinalis). These plants are known for their positive effects in folk medicine. The SPME-GC-MS method was chosen to characterize the fragrances. During the identification of volatile compounds, emphasis was put on suspected fragrance allergens listed in EC Regulation 1223/2009 in Annex III. Overall 106 compounds were identified together with 8 allergens in lemon balm, pot marigold contained 104 compounds with 7 allergens included, 82 compounds including 5 allergens in clary sage, 73 compounds were identified in milk thistle including 6 allergens and 110 compounds with 9 allergens included in goat’s rue.
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Vliv elicitorů, hnojení a technologie pěstování Ostropestřce mariánského (Silybum marianum L) na produkt a jeho využití
GUBIŠOVÁ, Jana
Milk Thistle (Silybum marianum) has been one of the best known medici herb for a very long time. Seeds contain biologicky aktive substances taxifoiln, silychristin, silydianin, silybin A, silybin B, isosilybin A and isosilybin B, commonly known as silymarin complex. The aim of this thesis was the influence of elicitors, fertilization and growing technology on this product and its utilizaion. In the first part I focused on history, botanic attributes, agrotechnology, chemical composition and substance efficiency method of determinativ and also on pharmacological onpacts of effective substances. In the practical part I conducted twho small parcel experiments to reveal the impal of elicitors on effective substances. I used two elicitors NanoFYTSi? in 1ml/l concentrantion and N-FENOLMIX? in 0.5ml/l concentration. In the second experiment from my supervisor elicitor NanoFYT Si? in 1ml/l concentraiton and N-FENOL MIX? in 0.5ml/l and elicitor ASA were used in three different concentration: low [10-5 mol/l], medium [ 10-4 mol/l] and high [10-3 mol/l]. In the conclusion I did statistical analysis of the above montioned experiments and compared them with other Publisher experiments. Then I propřed the growing technology and utilization of Milk Thistle.
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Growing technology of milk thistle (Silybum marianum L. Gaertn.) and fertilization with the aim of maximizing the quality of the product and its use
GUBIŠOVÁ, Jana
Milk thistle (Silybum marianum L. Gaertn.) a medicinal plant known for thousands years. In the Czech Republic is purposely planted since the 70s of last century. Last few years increased worldwide interest about this plant mainly due to the pharmaceutical industry, which uses isolated of the active ingredients. The advantage of this plant is low demands on environment and high yields if suitable agrotechnics aspects and soil quality are provided. The main objective of this thesis was to gather available information about milk thistle, growing technology, and familiarity with its botanical characteristics, chemical composition and active ingredients, farming techniques, use, fertilization and past management. Furthermore the study was focused on summarizing the methods for the determination of certain active substances clarification the pharmacological effects and the influence of elicitation and cultivation techniques for the active substances. In the conclusion was summarized the problem of the cultivation of milk thistle and technology designed for growing in practice and of its use. Especially as a technology of soil preparation, choosing suitable proceeding crop, seeding, fertilizing and the most demanding plant harvest. Furthermore there was given a proposal for the use of seeds of milk thistle in the food industry, where is considered as insufficient. The potential of this medicinal plant would be for example for the production of functional foods.
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The influence of the seed treatment on the milk thistle achene yield
HRDLIČKOVÁ, Helena
Milk thistle (Silybum marianum (L.) Gaertn.) is an ancient medicinal plant valued for its content of silymarin complex. These substances are albe to protect liver cells from damage by toxic substances. The aim was to assess the potential effect of milk thistle seed treatment (thermal water, Vitavax and Polyversum) on plant health and thus the seed yield of the crop by the form of small-area experiment. I have focused on plant height, number of plants per a square meter, number of inflorescencae per plant, number of achenes in inflorescencae, TGW, weight biomass yield and germination of produced achenes. During growth there was observed the incidence of diseases and pests. The results show a positive effect on the development and yield of achenes.
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