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Evaluation of chemical composition of jerusalem artichoke tubers from autumn and spring harvest
BRABCOVÁ, Anna
This bachalor´s thesis deals with the evaluation of the chemical composition of tubers of Jerusalem artichoke for the selected set of 8 clones (Lola, Refla, Gigant, Běloslupký, Úrodný, Völkenroder Spindel, C 63, Karina). Due to the possibility of overwintering tubers in the soil, the tubers were analyzed genotypes of autumn (when dry above-ground plant matter) and then spring harvest (before the germination of tubers) were searched and any changes due to the influence of the winter. The tubers of all genotypes were analyzed for dry matter, protein content, crude protein content and the amount of nitrogen-free substances. Further, an analysis of protein profile by SDS-PAGE method. The highest dry matter content has been determined in tubers clone Gigant (29.1%) and the lowest dry matter content has been determined in tubers clone Lola (19.9%). Due to wintering have been decreased dry matter, crude protein, protein and nitrogen-free substances in the tubers of most the clones.
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Evaluation of cultivar effect on tuber yield structure and selected parameters of tubers in Jerusalem Artichoke (\kur{Helianthus tuberosus} L.)
VANĚČEK, Petr
The aim of this bachelor's thesis was the evaluation of the effect of the Jerusalem artichoke´s (Helianthus tuberosus L.) genotype (clone) on yield structure and selected parameters of the tubers. A one-year field experiment was established on 25th April 2012 on the experimental station of the University of South Bohemia in the České Budějovice (altitude 395 m, a cereal production area), where 8 different genotypes (Refla, Lola, Gigant, Karina, Völkenroder Spindel, Úrodný, Běloslupký, C 63) were planted in 3 replications (spacing at 0.3 x 0.75 m). Úrodný and Gigant were evaluated as the earliest clones (appearing of the flower head 84 days after the date of the planting). The tubers of 5 consecutive plants from the middle row in each replication were harvested in autumn (on 31st October). All clones except clone Běloslupký (due to late planting) were statistically evaluated. The yield values moved in the range of 33.48 t/ha (C 63) to 44.74 t/ha (Úrodný). Genotype Völkenroder Spindel produced the most of the tubers per plant (average 24.3), but we identified the smallest average weight of the tubers (34.73 g) at this clone (R = 0.84%). The least tubers were produced by clone C 63 (16.3). The highest average weight of the tubers was achieved by genotype Úrodný (73.59 g; the 2nd place belongs to clone C 63 55.63 g). We used the method of the lyophilization (Alpha 1 4 LSC) to determine the dry matter content in tubers. The values of the dry matter content ranged from 18.85 % (Lola) to 22.36 % (Völkenroder Spindel). The quantity of the proteins and nitrogen contained in the dry matter were other parameters which we analyzed (BCA Protein Assay Kit, Pierce). The highest values of the proteins contained in the dry matter were obtained at the genotype Refla (6.13 %) and the lowest at the genotype Úrodný (3.90 %). The values of the nitrogen content moved in the range of 0.68 % (Lola) to 1.02 % (C 63). When we used methods of the SDS PAGE and the automatic chip electrophoresis we determined only quantitative differences of the proteins composition in the tubers. The approximate molecular weights of the identified proteins were 8, 20 30, 35, 40, 50, 65, 80, 85 a 90 kDa.
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