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Monoclonal gammopathies from point of view of the biochemist
Drábková, Gabriela ; Herink, Josef (advisor) ; Kovařík, Miroslav (referee)
Gabriela Drábková Monoclonal gammopathy from the point of view of a biochemist Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Healthcare bioanalytics- Specialist in laboratory methods Monoclonal gammopathy are a very heterogeneous group of diseases characterized by the proliferation of one clone of differentiated B lymphocytes producing homogeneous immunoglobulin, the so-called monoclonal immunoglobulin. This monoclonal immunoglobulin can be detected in serum and / or urine. Monoclonal gammopathy can also be defined as a group of diseases that result from mutation in a B-cell line. The monoclonal immunoglobulin may consist of both the intact immunoglobulin molecule or the structural components, i.e., the kappa or lambda light chains, more rarely from the heavy chains of the immunoglobulin molecule. The basic investigation methods of monoclonal immunoglobulin detection include serum protein electrophoresis and immunofixation electrophoresis. Another important method in diagnosis and monitoring is the determination of free light chains. Multiple myeloma is an example of a tumor in which the prognosis has improved so much in recent years that today we can talk about a chronic illness. Median survival is currently around six years, and some patients get into...
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Complete differential diagnostics of monoklonal gammopathy
MERVARTOVÁ, Ivana
The aim of the thesisis a quantitative detection of the monoclonal paraprotein in patients serum, eventually a qualitative detection in urine in group of hematological pacients examined at the Department of clinical biochemistry in Hospital Písek a.s. with electrophoretic analytical method (ELFO) and its subsequent specification by immunofixation electrophoresis (IFE). The quantification of free light chains (VLR), kappa and lambda, from the serum is made on some patients for more complex information, and it is made by immunoanalytic method. Futhermore immunoglobulins as well as values of albumin and values of total protein are quantitatively established in the serum, and these values can contribute to more precise diagnosis in case of finding of monoclonal components. From the stated hypothesis I conclude, that the patients with pathological values VLR and their ratios, which lead to diagnosis of the monoclonal gammopathy, will also have positive finding of monoclonal immunoglobulin on ELFO. The finding will be afterwards specified by IFE method and so the diagnosis of monoclonal gammopathy will be confirmed.
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The influence of the quality of the colostrum on the growth and health of calves
BERÁNKOVÁ, Anna
Bachelor thesis presents a summary of the importance of colostrum, its composition and compared to mature milk. Colostrum contains important proteins that ensure the health status of the calf. Total protein in colostrum contains important life immunoglobulins, which are involved in immunity calves. For quality connection and future health of calves, you need timely connection quality colostrum and calf submit a corresponding amount. Another important stage in the life of the calf, contributing to good growth and health, the feed technique, which is described in the relevant literature review section. There is also characterized by the meaning and role of colostral immunoglobulin. The methodology applied by methods of measuring the quality of colostrum and measuring total protein in blood serum from calves. In my own research were processed by the information provided by the company VHD Hradiště. Here, the work was focused on the maternal calves and influences the quality of the colostrum to calves and further quantities of immunoglobulins absorbed into the bloodstream after connecting colostrum. Selected influences on the quality of colostrum, calves health and their weight are processed into tables and graphs. The results were no statistically significant differences.
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Modifikované peptidy ve spojení s kvantovými tečkami
Janů, Libor
In this study, water soluble CdTe QDs of different emission wavelengths were prepared. Microwave synthesis was used to prepare CdTe QDs coated with mercaptopropionic acid (MPA). MPA ensures biocompatibility and good water solubility of QDs. Therefore QDs can be used directly in bioanalytics. Further, QDs modified with biotinylated glutathione (GSH) were prepared. In this case mercapto group (--SH) ensures a bonding of GSH to QDs surface and biotin serves as high specific linker for streptavidin, avidin or neutravidin modified analyte. Finally, peptide mediated immuno-quantum dots were prepared. CdTe QDs were coated with heptamer HWRGWVC which is able to bind Fc region of human immunoglobulin G.
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