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Functional and pathological changes in the stomach of hosts infected with gastric cryptosporidia
MARKOVÁ, Marie
The aim of this study was to observe and record the effect of infection with gastric species of the genus Cryptosporidium on the gastric characteristics of their hosts. The species Cryptosporidium proliferans was used for a series of experimental infections, and three types of model hosts - BALB/c mice, C57Bl6 mice and multimammate mice (Mastomys coucha) - were used as experimental animals. Oocysts were detected in the faeces of infected animals by standard parasitological, specific staining and molecular methods and the course of infection and pathogenicity of the Cryptosporidium species were described. Groups of animals comprising of 1-3 animals were humanely euthanized at seven-day intervals up to 70 days after infection. C. proliferans was found to have a direct effect on pH change in selected host types, with an increase in pH at the peak of infection and, depending on the host type, either an increase until the end of the experiment or, in the case of self-healing, a gradual return to physiological values. Pathological changes of the gastric mucosa during the course of the infection were recorded by histological methods, which were its thickening and increased mucus production, as well as the presence of inflammatory infiltrates and the intensity of the infection, to varying degrees across the selected hosts and the course of the infection. We found that even with short-term infection in BALB/c and C57Bl6 mice, there is both mucosal enlargement and thickening, although the pathological changes in these types of mice are not as extensive as in multimammate mice, which do not self-heal.
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Faktory ovlivňující excystaci žaludečních kryptosporidií hlodavců
SCHULZOVÁ, Tereza
The aim of this study was to determine the effect of host body temperature and en-vironmental pH on the excystation and preservation of the ability to infection of rodent gastric cryptosporidia oocysts. The infectivity of unexcysted oocysts was further examined. Immunodeficiency SCID mice (Mus musculus) were infected with C. proliferans oocysts, then their faeces were collected and individual experi-ments were performed. The effect of temperature on excystation, when oocysts were placed in water baths at the appropriate temperature (32-44 °C) and the effect of pH, when oocysts were exposed to solutions of different pH (2-7), was investi-gated. Cell separation was then performed using a flow cytometer and the values obtained were recorded in the tables. The most oocysts excysted at 40 °C and pH 7. An autopsy of infected mice was also performed to determine the infectivity of oo-cysts from various parts of the digestive system. All taken oocyst samples were infectious for the subsequent host. Infection experiments, which were further car-ried out revealed, that unexcysted oocysts exposed to hypochlorite and laboratory temperatures retain their ability to infect. In contrast, the oocyst sporozoites that excysted were susceptible to both hypochlorite and room temperature, thus losing the ability infect the subsequent host.
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Hlodavci jako rezervoár hantavirů
KAMIŠ, Jan
This study reveals the presence of hantaviruses in free-living rodents and insectivores in urban areas in the Czech Republic. A large number of hantavirus reservoir hosts were tested for hantavirus RNA in different tissues, using universal and specific primers for amplification of the large and medium fragments of hantavirus genomic RNA. Phylogenetic relationships of obtained nucleotide sequences of hantaviruses were reconstructed. Four different species of hantaviruses were detected, including two species pathogenic (or potentially pathogenic) for humans, suggesting a threat for public health. Additionally, inter-family spillover infections and hantavirus species-associated tissue tropism were recorded in rodent hosts.
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Laboratorní test disasortativního párování u hraboše polního - literární rešerše
BÍLKOVÁ, Pavlína
Petrásková P. (2019): Laboratorní test disasortativního párování u hraboše polního - literární rešerše [Laboratory test of disassortative pairing in the common vole - literature search. Bc. Thesis in Czech]-49 pp. , Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic. The aim of this work is to use the literature research to map current knowledge about the effect of odour preference on reproductive success in mammals focusing on voles. As assortative pairing is referred to each form of non-random pairing. Most of the time is meant a situation in which individuals who have similar phenotypes pair more often. The opposite is the situation where individuals with different phenotypes pair more often. This is sometimes referred to as negative assortative or disassortative pairing. In particular, this study deals with non-random pairing among to body odour . These are mainly linked to genes for the major histocompatibility complex (MHC) in animals, which play a key role in the immune system. MHC gene products found in various body secretions form the specific odour of each individual. In the research part were searched question for following questions: How is the breeding partner chosen? Is Assortative or Disassortative Pairing More Frequent? What information does body odour convey? What channels is the odour information transmitted and received? How is odour preference testing solved in laboratory conditions? In the practical part, a plan of the experiment is presented in which T-labyrinth will play the main role in determining the odour preference. Under laboratory conditions, voles will have plenty of time to complete the process
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Optimization of litter size in rodents
Cingelová, Alexandra ; Dušek, Adam (advisor) ; Sedláček, František (referee)
Optimization of litter size can be defined as adjustment of litter size according to actual environmental conditions or condition of mother to reach a compromise between the maximum number of offspring and their individual fitness. Rodents are an ideal taxon to study this topic because of their enormous interspecific variability in life strategies across the whole order. The variation in litter size among rodent species was influenced by countless ecological and social factors during their evolution. These include the geographical location of the breeding populations, various life and reproductive strategies such as r-/K-strategy, the presence of altricial or precocious offspring, various ways of life, the presence of water in the environment, predation rate, hibernation, and sociality. Litter size can be adjusted already before fertilization, for example by choosing a partner, by timing reproduction, condition, age and social status of the mother. Genetic factors affecting litter size are, for example, MHC glycoprotein polymorphism and t-haplotype. Reduction of the litter size may occur before insemination by influencing the ovulation rate, female reproductive hormone levels and exposure to the stressors, during implantation by selective abortion, and postpartum by infanticide, siblicide, and...
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Adaptive radiation of the genus Rattus
Skalíková, Hana ; Frynta, Daniel (advisor) ; Mazoch, Vladimír (referee)
Rats are an important group of rodents originating and living mainly in southeast Asia. They are important pests and reservoirs of zoonosis. Yet, about many species we only have basic information. Here, I summarized available information about 66 species of the genus Rattus and 10 species of the genus Bandicota, Diplothrix, Limnomys, Nesokia and Tarsomys. I focused on distribution, ecology and morphology (body lenght, tail lenght, hind food lenght and ear lenght) and their mutual relationships. The body lenght differs beetwen phylogenetic lineages and beetwen habitats. The other morphological characters (tail lenght, hind food lenght and ear lenght) correlated with body lenght. Further, I describe an adaptive radiation of rats. Rats are separated into several lineages, diferring in their geographic distribution and ecology. Moreover, many species are commensal, that is benefiting from close relationship with humans. Commensalism species can be found in several lineages of rats, therefore commensalism probably developped independently more than once. The commencalism was origined several times. I discuse why are some commensal species more successful than others. Key words: adaptive radiation, Bandicota, commensal species, ecomorphology, Rattus, rodents, southeast Asia
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Adaptive radiation of the genus Rattus
Skalíková, Hana ; Frynta, Daniel (advisor) ; Mazoch, Vladimír (referee)
Rats are an important group of rodents originating and living mainly in southeast Asia. They are important pests and reservoirs of zoonosis. Yet, about many species we only have basic information. Here, I summarized available information about 66 species of the genus Rattus and 10 species of the genus Bandicota, Diplothrix, Limnomys, Nesokia and Tarsomys. I focused on distribution, ecology and morphology (body lenght, tail lenght, hind food lenght and ear lenght) and their mutual relationships. The body lenght differs beetwen phylogenetic lineages and beetwen habitats. The other morphological characters (tail lenght, hind food lenght and ear lenght) correlated with body lenght. Further, I describe an adaptive radiation of rats. Rats are separated into several lineages, diferring in their geographic distribution and ecology. Moreover, many species are commensal, that is benefiting from close relationship with humans. Commensalism species can be found in several lineages of rats, therefore commensalism probably developped independently more than once. The commencalism was origined several times. I discuse why are some commensal species more successful than others. Key words: adaptive radiation, Bandicota, commensal species, ecomorphology, Rattus, rodents, southeast Asia
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The role of commensalism on vertical activity in exploration tests: a comparison of 12 populations of the genus Acomys
Štolhoferová, Iveta ; Frynta, Daniel (advisor) ; Špinka, Marek (referee)
The objective of this work was to investigate whether there are differences between commensal and non-commensal populations of spiny mice (Acomys spp.). There is a good evidence that some populations of Acomys cahirinus have been commensal since the time of ancient Egypt, that is for at least 3,500 years. Therefore, it could be expected that some adaptation for a commensal way of life have evolved. To find out, I tested twelve populations of spiny mice in two types of open field test - a vertical test and a hole board test. In the vertical test, a wire mesh was added and offered to spiny mice to climb on. Since human settlements represent an environment with a new predation pressure as well as new hiding opportunities like those in heights above the ground, I hypothesized that commensal populations of A. cahirinus would (1) explore less on the ground and (2) prefer to spend more time on the wire mesh than the non-commensal populations. Results supported the first hypothesis as in both tests commensal spiny mice spent less time exploring on the ground than other populations. The second hypothesis, however, was not supported - all populations showed approximately equal interest in the wire mesh and commensal spiny mice did not spend more time on it compared to the others. Nevertheless, an overall...
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Využití pokusných zvířat v ČR
MRÁZKOVÁ, Lucie
The animal experiments seem to be current theme of these days. This thesis is focused on using animals, especially rodents, in the Czech Republic for the last ten years. There is also emphasis on the legal principles of animal protection and observance of three "R" principles. In this study there were used data from available sources about using laboratory animals in the Czech Republic. Also there was prepared a questionnaire research in different scientific institutions which use animal models in their researches. There were found out from available sources that the amount of experimental animals is on the same level (about 230 000) during the years 2006-2015. There were no deflection. Also this fact is the same for using rodents (about 120 000) as biomodels. Further there was found out that the laboratory mouse (Mus musculus var. alba) is the most used experimental model. 66,7% of questioned respondents considered the laboratory mouse the most common used laboratory animal and 60% of questioned respondents supposed that the number of used laboratoty animals was decreasing.
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