|
|
Isolation and study of proteins with zona pellucida binding activity
Zigo, Michal
Binding of sperm to the oocyte is mediated by complementary molecules on the surface of both gametes and involves the interaction of sperm protein receptors with the zona pellucida (ZP) saccharide structures. It has been shown that many proteins receptors are involved in the sperm-ZP interaction, and potential primary sperm receptors for ZP glycoproteins have been investigated in various mammals. The majority of proteins with identified sperm-ZP binding activity belong to the plasma membrane proteins. However, the exact methods for isolation of sperm membrane proteins are still to be standardized. This study is focused on investigating how employment of various isolation protocols leads to acquisition of various protein mixtures. Further in the work, two possible approaches towards identification of potential ZP-binding partners are implemented, in order to disclose novel primary ZP-binding receptor candidates. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by: Triton X-100, Triton X-114, acetic acid, sodium dodecyl sulphate (SDS), N-octyl-β-D- glucopyranoside (OBG), rehydration buffer (RHB), and finally by freezing-thawing extraction and they were characterized by 1-D, 2-D protein profiles, glycoprotein staining and substrate zymographic methods. The results have...
|
|
|
Breeding of the Duroc breed in the Czech Republic and abroad
DVOŘÁKOVÁ, Lenka
The aim of this thesis was to evaluate the breeding of the Duroc breed in the Czech Republic. The thesis was focused on the development and changes in this population from 2000 to 2013. The thesis was also focused on reproductive performance and development of production traits based on the tests for individual performance. The work includes the formation of the Duroc breed, its characteristics, breeding standard and breeding objective. There is also handled the number of inmates, the number of farrows and of piglets born, the number of surviving piglets in 21 days, farrow weight, the length of the interim and loss of piglets in the performance tests. In the tests for individual performance here is handled the development of the average daily gain, lean meat and fat number with gilts and male pigs.
|
|
| |
guest ::
