|
|
Fiber and its consumption on a hospital bed
KŘÍŽKOVÁ, Ivana
The theme of the bachelor thesis is Fiber and its consumption on a hospital bed. Dietary fiber is defined as a carbohydrate food ingredient that cannot be transformed by one´s own enzymes. Fiber was originally considered a ballast food component. Gradually, it was found that it is a part of food fermented by colonic bacteria. Fiber is substrate for these bacteria and they transform it into substances intended for human organism. Fiber can be divided into soluble and insoluble. Both types are essential for human nutrition. A recommended fiber intake for an adult is 30 g per day. In the practical part of the bachelor thesis, was used qualitative research method. The research sample consisted of hospital two-weeks menus. One diet was applied in the hospital facility in the Central Bohemian region and one in the South Bohemian region. Using a computer program "Nutriservis Professional" I calculated the fiber content in the individual meals. The findings were compared with the recommended daily intake published in the book called Referenční hodnoty pro příjem živin and S výživou po celý rok. I also calculated energy value, amount of proteins, fats and carbohydrates of the menus. The results were compared with the recommended values. As a research I interviewed the nutritional therapists in the hospital facilities about the patient´s diet and possible solutions to health problems caused by a lack of fiber. The interviews consisted of eight open questions focused on the fiber consumption, denying of foods containing fiber. The results of calculation previewed the menu at the first hospital facility that fiber deficiency occurs primarily in a liquid diet, while for rational diet, diabetic diet and for infants and older children the fiber content is sufficient. The menu of the other hospital contains insufficient amount of fiber, especially for diabetic diet or geriatic diet reducing fat. On the other hand, diabetic diet contains a plenty of fiber. The interviews that were supposed to find out how nutritional therapists approch the fiber issues in diets brought similar responses. The hospital in the Central Bohemian region include into the diet especially fruit, vegetable, whole grain products and legumes. Older patients ages constipation suffering from bowel obstuction, intestinal disease and flatulence frequently refuse fiber, especially legumes. If a patient has health problems caused be the lack of fiber nutritional therapists usually solve this problem by education and inclusion of fruit, vegetable and whole meal bread into the patient's diet. The hospital in the South Bohemian region frequently include into the diet potatoes, rice, fruit, legumes, vegetable and whole grain breads. Fiber is often rejected by older people and people suffering from diarrheal diseases. Health problems resulting from the lack of fiber are often solved by recommending by foods containing fiber or the nutritional supplements such as NutriFibre.
|
|
|
Identification of selected probiotic bacteria in food additives
Bubeníková, Lucia ; Španová, Alena (referee) ; Rittich, Bohuslav (advisor)
Bifidobacteria and lactobacilli are gram-positive bacteria which belong to the lactic acid bacteria group. They are ordinary constituents of gastrointestinal microflora, play a pivotal role in human nutrition and health, prevent pathogen colonization and maintain normal mucosal immunity. In present days they are used in production of functional food products and pharmaceutical additives. Polymerase chain reaction (PCR) is used to the detection and identification of bacteria which belong to the genus of Bifidobacterium and Lactobacillus. Specific primers for each genus are used in PCR reactions for in vitro amplification of definite part of DNA. In this work, total DNA was isolated from three additives (Pangamin Bifi plus, Biopron Junior and Probiodom) by the method of phenol extraction and amplified using genus-specific PCR primers giving amplicons of 523 bp (Bifidobacterium) and 250 bp (Lactobacillus). Occurrence of bacteria of both genera was proved in all of the three tested probiotic products.
|
guest ::
