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In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus II
Pchálková, Tereza ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Tereza Pchálková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds active against fast growing strains of Mycobacterium genus II Key words: Mycobacteria, Tuberculosis, Antituberculotics, Microdilution broth method, Minimum inhibition concentration Background: The aim of this diploma thesis has been screening of in vitro antimycobacterial activity of novel compounds against fast growing strains of the genus Mycobacterium (Mycobacterium smegmatis and Mycobacterium aurum). Another aim has been predicting the structure-activity relationships for tested compounds. Methods: The technique used for activity determination was microdilution broth method. The value of minimum inhibition concentration for each compound was determined. The evaluation was performed visually and spectrophotometrically using the Alamar blue indicator. Results: A total of 79 compounds were tested. 22 tested compounds showed significant activity against mycobacteria. The substances were sorted according to the similarity in chemical structure into 10 groups. From a chemical point of view, these...
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In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus II
Pchálková, Tereza ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Bc. Tereza Pchálková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds active against fast growing strains of Mycobacterium genus II Key words: Mycobacteria, Tuberculosis, Antituberculotics, Microdilution broth method, Minimum inhibition concentration Background: The aim of this diploma thesis has been screening of in vitro antimycobacterial activity of novel compounds against fast growing strains of the genus Mycobacterium (Mycobacterium smegmatis and Mycobacterium aurum). Another aim has been predicting the structure-activity relationships for tested compounds. Methods: The technique used for activity determination was microdilution broth method. The value of minimum inhibition concentration for each compound was determined. The evaluation was performed visually and spectrophotometrically using the Alamar blue indicator. Results: A total of 79 compounds were tested. 22 tested compounds showed significant activity against mycobacteria. The substances were sorted according to the similarity in chemical structure into 10 groups. From a chemical point of view, these...
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In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus
Lyčková, Lenka ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Specialist in Laboratory Methods Candidate: Bc. Lenka Lyčková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus The aim of this work is to determine the efficacy of new compounds for possible treatment of tuberculosis in the future. A microdilution broth method with already proven procedure on Mycobacterium aurum and Mycobacterium smegmatis strains was used to determine the minimum inhibitory concentration of compounds. The theoretical part summarizes the epidemiological situation of recent years with global trends in the incidence and mortality of tuberculosis in the world and in the Czech Republic, including prospective plans for remediation in the time horizon. Mycobacteria are described in the main representation with M. tuberculosis. Tuberculosis disease - its history, forms, diagnosis and treatment with antituberculotics, which are divided according to the use lines, new drugs and drugs in development. The experimental part contains qualitative and quantitative methods of testing mycobacteria and captures the microdilution broth method used in...
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Antituberculotic susceptibility testing of Mycobacterium tuberculosis isolates
SUCHÁ, Denisa
Mycobacterium tuberculosis was first described by a German physician and microbiologist Robert Koch in the year 1882. It is an obligate pathogen, which causes a very serious infectious disease - tuberculosis. This disease is an important epidemiological problem all over the world. It is necessary to diagnose all the clinical cases of the disease, isolate the patiens and apply an adequate treatment to reduce the incidence of the disease and to prevent its spread. Essential for the diagnosis of tuberculosis is a confirmation by cultivation, i.e. isolation of Mycobacterium tuberculosis from clinical specimens, exact identification of the isolates and antitubercular drug susceptibility testing. The theme of this thesis was to determine the susceptibility to the basic anti-tubercular agents (streptomycin, isoniazid, rifampicin, ethambutol and pyrazinamide). The theoretical part provides an overview of susceptibility testing methods. A group of 275 isolates of Mycobacterium tuberculosis isolated from January 2008 to June 2013 from clinical specimens, in the Department of Microbiology of the Faculty Hospital in Pilsen, the Laboratory for Diagnosis of Mycobacteria was examined within the practical part. The susceptibility to antitubercular drugs was tested in all the isolates by two methods the proportion method (Canetti, 1963) and the method in semi-closed system BACTEC? MGITÔ 960. The results showed 100% concordance between both methods. The resistance was proved to at least one of the basic antitubercular drugs in 24 of tested isolates. The file was also evaluated from the demographical and epidemiological point of view. There was a higher incidence of tuberculosis among men (75% men and 25% women). In terms of age, the highest incidence has been shown between 40 and 60 years of age. The percentage of foreigners in the investigated group was 30 %. The Czech Republic is a country with a low incidence of tuberculosis,however, this situation can not be underestimated, if only due to the fact that there is a constant migration of people into the Czech Republic from the countries with a high incidence of tuberculosis and its drug-resistant strains.
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